Unburned Tobacco Smoke Affects Neuroinflammation-Related Pathways in the Rat Mesolimbic System
Abstract
:1. Introduction
2. Results
2.1. Gene Expression Analysis
2.2. Protein Analysis
3. Discussion
4. Materials and Methods
4.1. Animal Model and Nicotine Exposure
4.2. RNA Extraction and Gene Expression Analysis Using Real-Time qPCR
- −
- GAPDH Forward 5′-AGACAGCCGCATCTTCTTGT-3′;Reverse 5′-CTTGCCGTGGGTAGAGTCAT-3′;
- −
- KDM6A Forward 5′-TTTGGTCTACTTCCATTACAATGCA-3′;Reverse 5′-AAGCCCAAGTCGTAAATGAATTTC-3′;
- −
- KDM6B Forward 5′-ACCGCCTGCGTGCCTTAC-3′;Reverse 5′-GTGTTGCTGCTGCTGCTACTG-3′;
- −
- PTEN Forward 5′-TGGATTCGACTTAGACTTGACCT-3′;Reverse 5′-GCGGTGTCATAATGTCTCTCAG-3′;
- −
- PPARα Forward 5′-TGGAGTCCACGCATGTGAAG-3′;Reverse 5′-TTGTCGTACGCCAGCTTTAGC-3′;
- −
- PPARγ Forward 5′-CTGTTCGTACGCCAGCTTTAGC-3′;Reverse 5′-GCTCATATCTGTCTCCGTCTTCTT-3′;
4.3. SDS-Page and Immunoblotting
4.4. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Morosini, C.; Vivarelli, F.; Rullo, L.; Volino, E.; Losapio, L.M.; Paolini, M.; Romualdi, P.; Canistro, D.; Candeletti, S. Unburned Tobacco Smoke Affects Neuroinflammation-Related Pathways in the Rat Mesolimbic System. Int. J. Mol. Sci. 2024, 25, 5259. https://doi.org/10.3390/ijms25105259
Morosini C, Vivarelli F, Rullo L, Volino E, Losapio LM, Paolini M, Romualdi P, Canistro D, Candeletti S. Unburned Tobacco Smoke Affects Neuroinflammation-Related Pathways in the Rat Mesolimbic System. International Journal of Molecular Sciences. 2024; 25(10):5259. https://doi.org/10.3390/ijms25105259
Chicago/Turabian StyleMorosini, Camilla, Fabio Vivarelli, Laura Rullo, Emilia Volino, Loredana Maria Losapio, Moreno Paolini, Patrizia Romualdi, Donatella Canistro, and Sanzio Candeletti. 2024. "Unburned Tobacco Smoke Affects Neuroinflammation-Related Pathways in the Rat Mesolimbic System" International Journal of Molecular Sciences 25, no. 10: 5259. https://doi.org/10.3390/ijms25105259