Glucans with Different Degrees of Polymerization from Leuconostoc mesenteroides CICC6055: Analysis of Physicochemical Properties and Intestinal Prebiotic Function
Abstract
:1. Introduction
2. Results
2.1. Properties of Glucansucrase from L. mesenteroides 6055
2.2. Analyses of Enzyme Synthesis
2.2.1. TLC Analysis of Oligosaccharide Products
2.2.2. HPLC Analysis of Oligosaccharide Products
2.2.3. Comparison of Microbial Fermentation and Enzyme Synthesis
2.3. Analysis of Physicochemical Properties of Glucan and Oligoglucan
2.3.1. SEM, Zeta Potential, and Viscosity Analysis
2.3.2. Thermodynamic Analysis
2.3.3. Molecular Weight
2.3.4. XRD Analysis
2.3.5. Fourier Transform-InfraRed (FT-IR) Analysis
2.3.6. Nuclear Magnetic Resonance (NMR) Analysis
2.4. In Vitro Simulation of Intestinal Probiotic Function
2.4.1. Changes of pH and Strain Density during Fermentation
2.4.2. Production of SCFAs during Fermentation
2.4.3. Changes in Intestinal Microbial Flora Growth
3. Discussion
3.1. Properties of Glucansucrase from L. mesenteroides 6055
3.2. Analyses of Enzyme Synthesis
3.3. Analysis of Physicochemical Properties of Glucan and Oligoglucan
3.4. In Vitro Simulation of Intestinal Probiotic Function
4. Materials and Methods
4.1. Preparation and Cultivation of Bacterial Strains
4.2. Extraction of Exopolysaccharides by L. mesenteroides 6055
4.2.1. Glucans from Strains Fermentation
4.2.2. Analyses of Properties of Glucansucrase
Extraction, Purification, and Enzyme Activity Assay
Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Oligoglucans from Enzyme Synthesis
Thin-Layer Chromatography (TLC) Analysis
High-Performance Liquid Chromatography (HPLC)
4.3. Analysis of Physicochemical Properties of Glucan and Oligoglucan
4.3.1. Scanning Electron Microscopy (SEM)
4.3.2. Zeta Potential
4.3.3. Apparent Viscosity
4.3.4. Thermodynamic Properties
4.3.5. Molecular Weight Distribution
4.3.6. Crystalline Configuration
4.3.7. Fourier Transform-Infrared Spectroscopy (FT-IR)
4.3.8. Nuclear Magnetic Resonance (NMR)
4.4. Simulated Gastrointestinal Fermentation of Glucan and Oligoglucan
4.4.1. In Vitro Fermentation Procedure
4.4.2. Determination of pH, Strain Density, and Short-Chain Fatty Acids (SCFAs)
4.4.3. Identification of Intestinal Flora in Fermentation Broth
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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0–2000 | 2000–10,000 | >10,000 | Mn (Da) | Mw (Da) | PD | |
---|---|---|---|---|---|---|
Glucan | 0 | 0 | 100% | 844,190 | 1,117,981 | 1.32432 |
Oligoglucan | 93.3% | 6.483% | 0.187% | 725 | 3342 | 4.60966 |
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Gu, J.; Jiao, Z.; Wang, T.; Zhang, B.; Zhao, H. Glucans with Different Degrees of Polymerization from Leuconostoc mesenteroides CICC6055: Analysis of Physicochemical Properties and Intestinal Prebiotic Function. Int. J. Mol. Sci. 2024, 25, 258. https://doi.org/10.3390/ijms25010258
Gu J, Jiao Z, Wang T, Zhang B, Zhao H. Glucans with Different Degrees of Polymerization from Leuconostoc mesenteroides CICC6055: Analysis of Physicochemical Properties and Intestinal Prebiotic Function. International Journal of Molecular Sciences. 2024; 25(1):258. https://doi.org/10.3390/ijms25010258
Chicago/Turabian StyleGu, Jiabao, Ziyan Jiao, Tao Wang, Bolin Zhang, and Hongfei Zhao. 2024. "Glucans with Different Degrees of Polymerization from Leuconostoc mesenteroides CICC6055: Analysis of Physicochemical Properties and Intestinal Prebiotic Function" International Journal of Molecular Sciences 25, no. 1: 258. https://doi.org/10.3390/ijms25010258