Next Article in Journal
Viral Vector-Based Gene Therapy
Previous Article in Journal
Secreted Soluble Factors from Tumor-Activated Mesenchymal Stromal Cells Confer Platinum Chemoresistance to Ovarian Cancer Cells
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 24
Issue 9
10.3390/ijms24097733
Review Report
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Rapid and Sensitive Detection of Streptococcus iniae in Trachinotus ovatus Based on Multienzyme Isothermal Rapid Amplification

Int. J. Mol. Sci. 2023, 24(9), 7733; https://doi.org/10.3390/ijms24097733
by Yifen Wang 1,2,†, Jingjing Niu 1,†, Minmin Sun 1,2, Ziyi Li 1,2, Xiangyuan Wang 1,2, Yan He 1,2 and Jie Qi 1,2,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Reviewer 3: Anonymous
Int. J. Mol. Sci. 2023, 24(9), 7733; https://doi.org/10.3390/ijms24097733
Submission received: 24 March 2023 / Revised: 12 April 2023 / Accepted: 13 April 2023 / Published: 23 April 2023
(This article belongs to the Section Molecular Oncology)

Round 1

Reviewer 1 Report

The paper is well written and research well designed.

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report


Comments for author File: Comments.docx

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 3 Report

 The manuscript reported the research results of rapid and sensitive detection of Streptococcus iniae in Trachinotus ovatus based on multienzyme isothermal rapid amplification in detail. However, some major issues should be mentioned.

 (1)   Multienzyme isothermal rapid amplification is a homologous technology of recombinase polymerase amplification (RPA), while the reference 11&17 are not well cited, and the difference between the two methods was not explained in detail.

(2)   As a developing method in rapid diagnosis, RPA and MIRA are extensively studied. The main differences of the studies are the primers and detection targets. The novelty of the manuscript is pool and may not be interested to the readers to meet the high quality publishing on IJMS.

      Such as Rapid detection of Staphylococcus aureus using a novel multienzyme isothermal rapid amplification technique. Front. Microbiol. 2022, 13:1027785; Development of a multienzyme isothermal rapid amplification and lateral flow dipstick combination assay for bovine coronavirus detection.2023, Front. Vet. Sci. 9:1059934. Development and evaluation of a rapid and sensitive multienzyme isothermal rapid amplification with a lateral flow dipstick assay for detection of Acinetobacter baumannii in spiked blood specimens. Front. Cell. Infect, 2022, Microbiol. 12:1010201.

(3)   In the conclusion section, the manuscript reported that two methods were established in for rapid diagnosis of S. iniae, to be more more precisely, development and validation of the MIRA-LFD assay for detection of S. iniae can be addressed, or development and validation of the primers used for detection of S. iniae，other than established two methods.

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Round 2

Reviewer 3 Report

The author provided some in-depth explanations， and the manuscript can be accepted in present form.

Back to TopTop