The Synergistic Antimicrobial Effect and Mechanism of Nisin and Oxacillin against Methicillin-Resistant Staphylococcus aureus
Abstract
:1. Introduction
2. Results
2.1. The Synergy Antimicrobial Effect of OX + NIS against MRSA
2.1.1. Antimicrobial Effect against MRSA In Vitro
2.1.2. MIC and FICI of OX + NIS against MRSA
2.1.3. Effect on Growth Curve of MRSA Treated with OX + NIS
2.1.4. Effect on Biofilm Producing of MRSA Treated with OX + NIS
2.2. The Synergy Antimicrobial Mechanism of OX + NIS against MRSA
2.2.1. Morphological Changes of MRSA Cell
2.2.2. Integrity Changes of MRSA Cell Wall and Cell Membrane
2.2.3. Transcription Change of mecA in MRSA
2.3. Efficacy of OX + NIS in the Treatment of MRSA-Induced Wound Infections in Mice Skin
2.3.1. Effect of OX + NIS Treatment on Wound Closure
2.3.2. Histopathological Change Analysis of OX + NIS Treatment on Wound Closure
2.3.3. Inflammation Cytokines Change Analysis of OX + NIS Treatment on Wound Closure
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains, Antimicrobial Agents, and Culture Conditions
4.2. Minimal Inhibitory Concentration (MIC) Assay
4.3. Synergy Test of OX + NIS against MRSA
4.4. Growth of MRSA Incubated with OX + NIS
4.5. Biofilm Formation Assay
4.6. Microscopic Observation of MRSA Cells Morphological Changes Treated with OX + NIS
4.7. Extracellular Alkaline Phosphatase Activity of MRSA Strain Treated with OX + NIS
4.8. Membrane Permeability of MRSA Strain Treated with OX + NIS
4.9. Quantification of mecA Transcription of MRSA Strain Treated with OX + NIS
4.10. Murine Skin Infection Model with MRSA
4.10.1. Animal Used
4.10.2. Skin Wound Generation and Infection with MRSA
4.10.3. Treatment and Evaluation of MRSA Wound Infections
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | MIC (Alone) | MIC (Combination) | FICI | Inhibitory Effect | ||
---|---|---|---|---|---|---|
OX (μg/mL) | NIS (μg/mL) | OX (μg/mL) | NIS (μg/mL) | |||
Yn2020043 | 32 | 12,800 | 8 | 3200 | 0.500 | synergy |
Yn2020051 | 64 | 12,800 | 16 | 3200 | 0.500 | synergy |
Yn2020070 | 16 | 12,800 | 4 | 1600 | 0.375 | synergy |
ATCC25923 | 8 | 12,800 | 4 | 6400 | 1.000 | addition |
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Wang, J.; Ma, X.; Li, J.; Shi, L.; Liu, L.; Hou, X.; Jiang, S.; Li, P.; Lv, J.; Han, L.; et al. The Synergistic Antimicrobial Effect and Mechanism of Nisin and Oxacillin against Methicillin-Resistant Staphylococcus aureus. Int. J. Mol. Sci. 2023, 24, 6697. https://doi.org/10.3390/ijms24076697
Wang J, Ma X, Li J, Shi L, Liu L, Hou X, Jiang S, Li P, Lv J, Han L, et al. The Synergistic Antimicrobial Effect and Mechanism of Nisin and Oxacillin against Methicillin-Resistant Staphylococcus aureus. International Journal of Molecular Sciences. 2023; 24(7):6697. https://doi.org/10.3390/ijms24076697
Chicago/Turabian StyleWang, Jun, Xinxin Ma, Jing Li, Lu Shi, Lijuan Liu, Xinyao Hou, Sijin Jiang, Pu Li, Jia Lv, Lei Han, and et al. 2023. "The Synergistic Antimicrobial Effect and Mechanism of Nisin and Oxacillin against Methicillin-Resistant Staphylococcus aureus" International Journal of Molecular Sciences 24, no. 7: 6697. https://doi.org/10.3390/ijms24076697