A Putative Receptor for Ferritin in Mollusks: Characterization of the Insulin-like Growth Factor Type 1 Receptor
Abstract
:1. Introduction
2. Results
2.1. Identification of Potential Membrane Anchors for SbFn
2.2. Molecular Characterization of SbIGF-1R
2.3. The mRNA Expression Pattern of SbIGF-1R in Various Tissues
2.4. The Interaction Mode between SbIGF-1R and SbFn
3. Discussion
4. Materials and Methods
4.1. Animals and Sample Collection
4.2. His-Fusion Proteins and His Pull-Down Assays
4.3. Clone and Bioinformatic Analyses of SbIGF-1R
4.4. qPCR Assay of SbIGF-1R Expression
4.5. Preparation of Polyclonal Antibody
4.6. Western Blotting
4.7. Co-Locaalization Assay
4.8. Yeast Two-Hybrid Assay
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Primer Sequence (5′-3′) |
---|---|
Gene clone primer | |
SbFn-F | CTCCGAAACTCCGCCATATTCTCAC |
SbFn-R | CATGGAATTTATTTAGAAAAAGGACTG |
SbIGF-1R-F | ATGACACCACAAAGTGTGGCATCAG |
SbIGF-1R-R | TCAGCATGATGTCATCCTCATGTGT |
Real-time quantitative PCR primers | |
qSbIGF-1R-F | GAAGATCACTGCCGAAGAAGGA |
qSbIGF-1R-R | CGTGGAATGCATACAAGAGGTTTC |
qSbRL15-F | AGACCAGACAAAGCCAGAAGAC |
qSbRL15-R | GCTGAAGTAAGTCCACGCATT |
Vector construction primers | (The underline means restriction enzyme site) |
SbFn-BamH I | CGCGGATCCATGGCTCAAACACAACCAAG |
SbFn-Xho I | CCGCTCGAGTATATGTAGATAAAAGCCTAA |
SbIGF-1R-FNIII-BamH I | CGCGGATCCTATGTAACTCAACTCAACCTGACTA |
SbIGF-1R-FNIII-Xho I | CCGCTCGAGTCTAAAAATGTTTATATGATGTCCATTCT |
FnⅢ-α-BD-BamH I | CGCGGATCCGTTATGTAACTCAACTCAACCTGACTA |
FnⅢ-α-BD-Not I | AAGGAAAAAAGCGGCCGCTTTACGCCAATACACTTCGTAATGA |
FnⅢ-β-BD-BamH I | CGCGGATCCGTGCAGTCGATGTGAATGAAACTGAGG |
FnⅢ-β-BD-Not I | AAGGAAAAAAGCGGCCGCAAAATGTTTATATGATGTCCATTCT |
SbFn-AD-EcoR I | CCGGAATTCGCTCAAACACAACCAAGACAAAACT |
SbFn-AD-BamH I | CGCGGATCCCACTGCTCATGGTTTCCTTGTCATAC |
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Huang, B.; Liu, Q.; Bai, C.; Li, C.; Wang, C.; Xin, L. A Putative Receptor for Ferritin in Mollusks: Characterization of the Insulin-like Growth Factor Type 1 Receptor. Int. J. Mol. Sci. 2023, 24, 6175. https://doi.org/10.3390/ijms24076175
Huang B, Liu Q, Bai C, Li C, Wang C, Xin L. A Putative Receptor for Ferritin in Mollusks: Characterization of the Insulin-like Growth Factor Type 1 Receptor. International Journal of Molecular Sciences. 2023; 24(7):6175. https://doi.org/10.3390/ijms24076175
Chicago/Turabian StyleHuang, Bowen, Qin Liu, Changming Bai, Chen Li, Chongming Wang, and Lusheng Xin. 2023. "A Putative Receptor for Ferritin in Mollusks: Characterization of the Insulin-like Growth Factor Type 1 Receptor" International Journal of Molecular Sciences 24, no. 7: 6175. https://doi.org/10.3390/ijms24076175