ABCB1 and ABCC1 Function during TGF-β-Induced Epithelial-Mesenchymal Transition: Relationship between Multidrug Resistance and Tumor Progression
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Cell Subculture
4.2. Induction of Epithelial-Mesenchymal Transition
4.3. Induction of ABCB1 Transporter Expression
4.4. Antibody Labeling
4.5. ABCB1 and ABCC1-Mediated Efflux Assay
4.6. Assessment of Cellular Viability
4.7. Flow Cytometry Analysis
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
Abbreviations
ABC | ATP-binding cassette |
ABCB1 | subfamily B, member 1 |
ABCC1 | subfamily C, member 1 |
CSC | cancer stem cells |
EMT | epithelial-mesenchymal transition |
FN | fibronectin |
GSH | reduced glutathione |
GSSG | oxidized glutathione |
MDR | multidrug resistance |
MFI | median fluorescence intensities |
ROS | reactive oxygen species |
SP | side population |
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da Costa, K.M.; Freire-de-Lima, L.; da Fonseca, L.M.; Previato, J.O.; Mendonça-Previato, L.; Valente, R.d.C. ABCB1 and ABCC1 Function during TGF-β-Induced Epithelial-Mesenchymal Transition: Relationship between Multidrug Resistance and Tumor Progression. Int. J. Mol. Sci. 2023, 24, 6046. https://doi.org/10.3390/ijms24076046
da Costa KM, Freire-de-Lima L, da Fonseca LM, Previato JO, Mendonça-Previato L, Valente RdC. ABCB1 and ABCC1 Function during TGF-β-Induced Epithelial-Mesenchymal Transition: Relationship between Multidrug Resistance and Tumor Progression. International Journal of Molecular Sciences. 2023; 24(7):6046. https://doi.org/10.3390/ijms24076046
Chicago/Turabian Styleda Costa, Kelli Monteiro, Leonardo Freire-de-Lima, Leonardo Marques da Fonseca, José Osvaldo Previato, Lucia Mendonça-Previato, and Raphael do Carmo Valente. 2023. "ABCB1 and ABCC1 Function during TGF-β-Induced Epithelial-Mesenchymal Transition: Relationship between Multidrug Resistance and Tumor Progression" International Journal of Molecular Sciences 24, no. 7: 6046. https://doi.org/10.3390/ijms24076046