Protective Effect and Mechanism of Aspirin Eugenol Ester on Lipopolysaccharide-Induced Intestinal Barrier Injury
Abstract
:1. Introduction
2. Results
2.1. AEE Alleviated LPS-Induced Cytotoxicity
2.2. AEE Alleviated LPS-Induced Oxidative Stress
2.3. AEE Alleviated LPS-Induced Inflammatory Responses
2.4. Effects of AEE on the Morphology of LPS-Induced Caco-2 Cells
2.5. AEE Alleviated LPS-Induced Caco-2 Cell Monolayer Damage
2.6. AEE Inhibited LPS-Induced Downregulation of TJ Proteins
2.7. AEE Inhibited LPS-Induced NF-κB and MMP-9 Activation
2.8. Metabolomic Analysis of LPS-Induced Caco-2 Cells
2.8.1. Metabolite Analysis of Cell Lysates
2.8.2. Metabolic Pathway Analysis
2.9. AEE Pretreatment Alleviated LPS-Induced Colitis in Rats
2.10. AEE Improved Inflammation and Intestinal Barrier Damage in LPS-Induced Rats
2.11. AEE Improved the Gut Microbiota Structure in LPS-Treated Rats
2.11.1. Phylum Level
2.11.2. Genus Level
2.11.3. Species Level
2.11.4. Differential Species Analysis
2.11.5. Functional Level Analysis of Cecum Microbes by Metagenome
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture and Treatments
4.3. Cell Viability
4.4. AEE Treatment and LPS Stimulation
4.5. Measurement of Trans-Epithelial Monolayer Resistance
4.6. Measurement of Paracellular Permeability
4.7. Measurement of LDH, ROS, SOD, and GSH in Cell Supernatant
4.8. Protein Extraction and Western Blotting
4.9. Immunofluorescence
4.10. Metabolomics Analysis
4.10.1. Cell Collection and Processing
4.10.2. UPLC-QTOF-MS/MS Conditions
4.10.3. Metabolomics Data Analysis
4.11. Drug Preparation in the Animal Study
4.12. Animal Experiment
4.13. Measurement of Disease Activity Index (DAI) Scores and Immune Organ Index
4.14. Measurement of Inflammatory Factors in Serum and Cell Supernatant
4.15. Histopathological Analysis
4.16. RNA Extraction and RT-qPCR
4.17. Metagenome Sequencing for the Contents of the Cecum
4.17.1. DNA Extractions
4.17.2. DNA Library Construction
4.17.3. Data Analysis
4.18. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
AEE | Aspirin eugenol ester |
LPS | Lipopolysaccharide |
AP side | Side of the intestine |
BL side | Lower chamber is the basal side |
Papp | Apparent permeability coefficient |
LY | Lucifer yellow |
TEER | Transepithelial electrical resistance |
TJ | Tight junction |
DAI | Disease Activity Index |
ELISA | Enzyme-linked immunosorbent assay |
TNF-α | Tumor necrosis factor-α |
IL-6 | Interleukin-6 |
IL-1β | Interleukin-1β |
LDH | Lactate dehydrogenase |
GSH | Glutathione |
SOD | Superoxide dismutase |
NF-κB | Nuclear factor-kappa B |
MMP-9 | Matrix metalloproteinase-9 |
ROS | Reactive oxygen species |
COX | Cyclooxygenase |
LOX | Lipoxygenase |
LTs | Leukotrienes |
AA | Arachidonic acid |
HE | Hematoxylin–Eosin |
CCK-8 | Cell counting kit-8 |
BCA | Bicinchoninic acid |
CMC-Na | Carboxymethylcellulose |
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Tao, Q.; Liu, X.-W.; Zhang, Z.-D.; Ma, N.; Lu, X.-R.; Ge, W.-B.; Li, J.-Y.; Yang, Y.-J. Protective Effect and Mechanism of Aspirin Eugenol Ester on Lipopolysaccharide-Induced Intestinal Barrier Injury. Int. J. Mol. Sci. 2023, 24, 17434. https://doi.org/10.3390/ijms242417434
Tao Q, Liu X-W, Zhang Z-D, Ma N, Lu X-R, Ge W-B, Li J-Y, Yang Y-J. Protective Effect and Mechanism of Aspirin Eugenol Ester on Lipopolysaccharide-Induced Intestinal Barrier Injury. International Journal of Molecular Sciences. 2023; 24(24):17434. https://doi.org/10.3390/ijms242417434
Chicago/Turabian StyleTao, Qi, Xi-Wang Liu, Zhen-Dong Zhang, Ning Ma, Xiao-Rong Lu, Wen-Bo Ge, Jian-Yong Li, and Ya-Jun Yang. 2023. "Protective Effect and Mechanism of Aspirin Eugenol Ester on Lipopolysaccharide-Induced Intestinal Barrier Injury" International Journal of Molecular Sciences 24, no. 24: 17434. https://doi.org/10.3390/ijms242417434