Disrupting Poly(ADP-ribosyl)ating Pathway Creates Premalignant Conditions in Mammalian Liver
Abstract
:1. Introduction
2. Results
2.1. The loxP-Flanked Parg Gene Region Is Deleted in Hepatocytes of Mice Expressing the Albumin Promoter-Driven Cre Recombinase
2.2. The Regeneration Capacity of Parg Hepatocyte-Knockout Livers Is Not Compromised
2.3. Liver Development and Functioning in the Absence of PARG in Hepatocytes Is Affected
2.4. The Lack of PARG in Hepatocytes Predisposes Liver to Tumors
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Western Blotting
4.3. Chemically Induced Liver Tumor Generation
4.4. Tissue Histology and Immunofluorescence Staining
4.5. Nuclei Area Calculation in Liver Sections
4.6. Nuclei Isolation from Liver and Flow Cytometry
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Karpova, Y.; Orlicky, D.J.; Schmidt, E.E.; Tulin, A.V. Disrupting Poly(ADP-ribosyl)ating Pathway Creates Premalignant Conditions in Mammalian Liver. Int. J. Mol. Sci. 2023, 24, 17205. https://doi.org/10.3390/ijms242417205
Karpova Y, Orlicky DJ, Schmidt EE, Tulin AV. Disrupting Poly(ADP-ribosyl)ating Pathway Creates Premalignant Conditions in Mammalian Liver. International Journal of Molecular Sciences. 2023; 24(24):17205. https://doi.org/10.3390/ijms242417205
Chicago/Turabian StyleKarpova, Yaroslava, David J. Orlicky, Edward E. Schmidt, and Alexei V. Tulin. 2023. "Disrupting Poly(ADP-ribosyl)ating Pathway Creates Premalignant Conditions in Mammalian Liver" International Journal of Molecular Sciences 24, no. 24: 17205. https://doi.org/10.3390/ijms242417205