The Roles of the PSEUDO-RESPONSE REGULATORs in Circadian Clock and Flowering Time in Medicago truncatula
Abstract
:1. Introduction
2. Results
2.1. Identification of PRRs and Their Loss-of-Function Mutants in M. truncatula
2.2. Subcellular Localizations of MtPRR9, 7, and 5 and Expression Patterns
2.3. The Expression Patterns of Genes Associated with the Circadian Clock Are Altered in mtprr Mutants
2.4. MtPRR9, 7, and 5 Regulate Flowering Time
2.5. MtPRR9, 7, and 5 Physically Interact with MtTPL/MtTPR Proteins
2.6. MtPRR9, 7, and 5 Can Form Heterodimers
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Identification and Phylogenetic Analysis of MtPRRs
4.3. Conserved Domains Analysis
4.4. Subcellular Localization Analysis
4.5. RNA Extraction, RT-PCR, qRT-PCR, and Statistical Analysis
4.6. Double/Triple/Quadruple Mutant Generation
4.7. Y2H and BiFC Assays
4.8. Accession Numbers
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, X.; Zhang, J.; Liu, X.; Kong, Y.; Han, L. The Roles of the PSEUDO-RESPONSE REGULATORs in Circadian Clock and Flowering Time in Medicago truncatula. Int. J. Mol. Sci. 2023, 24, 16834. https://doi.org/10.3390/ijms242316834
Wang X, Zhang J, Liu X, Kong Y, Han L. The Roles of the PSEUDO-RESPONSE REGULATORs in Circadian Clock and Flowering Time in Medicago truncatula. International Journal of Molecular Sciences. 2023; 24(23):16834. https://doi.org/10.3390/ijms242316834
Chicago/Turabian StyleWang, Xiao, Juanjuan Zhang, Xiu Liu, Yiming Kong, and Lu Han. 2023. "The Roles of the PSEUDO-RESPONSE REGULATORs in Circadian Clock and Flowering Time in Medicago truncatula" International Journal of Molecular Sciences 24, no. 23: 16834. https://doi.org/10.3390/ijms242316834