Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems
Abstract
:1. Introduction
2. Results
2.1. Analysis of Sperm Chromatin Status
2.1.1. Chromatin Maturation
2.1.2. Chromatin Compaction
2.1.3. Chromatin Stability
2.1.4. Protamine Deficiency
2.2. Analysis of DNA Fragmentation
2.3. Correlation between Chromatin Status and DNA Fragmentation
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Animals
4.3. Sperm Collection
4.4. Aniline Blue Staining
4.5. Diff-Quik Staining
4.6. Toluidine Blue Staining
4.7. Chromomycin A3 Staining
4.8. DNA Fragmentation Analysis
4.9. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Percentage of Stained Cells at | |||
---|---|---|---|---|
0 h | 3 h | Δ3 h–0 h | ||
M. musculus | 0.55 ± 0.23 | 1.45 ± 0.42 | 0.90 ± 0.39 | |
M. spretus | 0.55 ± 0.36 | 0.90 ± 0.32 | 0.35 ± 0.52 | |
M. spicilegus | 0.00 ± 0.00 | 0.65 ± 0.26 | 0.65 ± 0.26 |
Species | Percentage of Stained Cells at | ||
---|---|---|---|
0 h | 3 h | Δ3 h–0 h | |
M. musculus | 1.15 ± 0.06 a | 2.25 ± 0.14 a | 1.10 ± 0.19 |
M. spretus | 0.70 ± 0.20 a | 1.95 ± 0.59 a | 1.25 ± 0.50 |
M. spicilegus | 0.50 ± 0.16 a | 1.75 ± 0.47 a | 1.25 ± 0.36 |
Completely Stained (%) | Partially Stained (%) | Total Staining (%) | Total Staining | ||||
---|---|---|---|---|---|---|---|
Species | 0 h | 3 h | 0 h | 3 h | 0 h | 3 h | Δ3 h–0 h |
M. musculus | 0.95 ± 0.20 | 1.40 ± 0.47 | 0.10 ± 0.06 | 0.40 ± 0.29 | 1.05 ± 0.18 | 1.80 ± 0.70 | 0.75 ± 0.68 |
M. spretus | 1.15 ± 0.36 | 1.80 ± 0.24 | 0.05 ± 0.05 | 0.25 ± 0.19 | 1.20 ± 0.40 | 2.05 ± 0.34 | 0.85 ± 0.56 |
M. spicilegus | 0.35 ± 0.17 | 1.35 ± 0.33 | 0.05 ± 0.05 | 0.60 ± 0.15 | 0.40 ± 0.19 a | 1.95 ± 0.40 a | 1.55 ± 0.35 |
Species | Parameter | 0 h | 3 h | Δ3 h–0 h |
---|---|---|---|---|
M. musculus | %tDFI | 1.38 ± 0.14 a,1,2 | 1.89 ± 0.19 a,1,2 | 0.51 ± 0.22 |
%HDS | 3.77 ± 0.51 | 6.98 ± 1.45 | 3.21 ± 1.24 | |
M. spretus | %tDFI | 0.56 ± 0.06 1 | 0.76 ± 0.17 1 | 0.19 ± 0.12 |
%HDS | 2.60 ± 0.25 | 7.84 ± 2.14 | 5.24 ± 2.07 | |
M. spicilegus | %tDFI | 0.35 ± 0.03 2 | 0.41 ± 0.05 2 | 0.06 ± 0.05 |
%HDS | 2.76 ± 0.48 | 6.86 ± 2.58 | 4.11 ± 2.24 |
Species | Parameter | tDFI | HDS | ||||
---|---|---|---|---|---|---|---|
0 h | 3 h | Δ3 h–0 h | 0 h | 3 h | Δ3 h–0 h | ||
All species | AB | 0.2925 (0.290) | 0.3679 (0.177) | 0.1002 (0.722) | 0.2562 (0.357) | 0.5450 (0.036) | 0.6345 (0.011) |
DQ | 0.6163 (0.014) | 0.2625 (0.345) | 0.0486 (0.864) | 0.6412 (0.010) | 0.5670 (0.028) | 0.6132 (0.015) | |
CMA3 | 0.2918 (0.291) | 0.0382 (0.892) | −0.0833 (0.768) | 0.3284 (0.232) | 0.5927 (0.020) | 0.4381 (0.102) | |
M. musculus | AB | 0.2858 (0.641) | −0.2360 (0.702) | −0.0253 (0.968) | 0.5228 (0.366) | 0.7169 (0.173) | 0.7819 (0.118) |
DQ | 0.4016 (0.503) | 0.6120 (0.273) | 0.4455 (0.452) | 0.4116 (0.491) | −0.0899 (0.886) | −0.0478 (0.939) | |
CMA3 | 0.5115 (0.378) | 0.4793 (0.412) | 0.3814 (0.526) | 0.4551 (0.441) | 0.9148 (0.030) | 0.8926 (0.042) | |
M. spretus | AB | −0.3486 (0.565) | 0.1641 (0.792) | −0.0292 (0.963) | 0.0717 (0.909) | 0.8115 (0.095) | 0.9833 (0.003) |
DQ | 0.0602 (0.923) | 0.2023 (0.744) | −0.0995 (0.874) | 0.5443 (0.343) | 0.5308 (0.357) | 0.6044 (0.280) | |
CMA3 | −0.0890 (0.887) | 0.0645 (0.918) | −0.510 (0.379) | 0.4174 (0.485) | 0.4768 (0.417) | 0.5130 (0.377) | |
M. spicilegus | AB | - | −0.1220 (0.845) | 0.4029 (0.501) | - | 0.4940 (0.398) | 0.4454 (0.452) |
DQ | −0.8715 (0.054) | −0.2552 (0.679) | 0.3730 (0.536) | 0.7893 (0.112) | 0.8107 (0.096) | 0.8480 (0.070) | |
CMA3 | −0.5783 (0.307) | −0.3926 (0.513) | −0.3614 (0.550) | 0.5568 (0.330) | 0.5962 (0.289) | 0.1483 (0.812) |
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Agudo-Rios, C.; Sanchez-Rodriguez, A.; Idrovo, I.I.D.; Laborda-Gomariz, J.Á.; Soler, A.J.; Teves, M.E.; Roldan, E.R.S. Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems. Int. J. Mol. Sci. 2023, 24, 15954. https://doi.org/10.3390/ijms242115954
Agudo-Rios C, Sanchez-Rodriguez A, Idrovo IID, Laborda-Gomariz JÁ, Soler AJ, Teves ME, Roldan ERS. Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems. International Journal of Molecular Sciences. 2023; 24(21):15954. https://doi.org/10.3390/ijms242115954
Chicago/Turabian StyleAgudo-Rios, Clara, Ana Sanchez-Rodriguez, Ingrid I. D. Idrovo, Juan Ángel Laborda-Gomariz, Ana J. Soler, Maria E. Teves, and Eduardo R. S. Roldan. 2023. "Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems" International Journal of Molecular Sciences 24, no. 21: 15954. https://doi.org/10.3390/ijms242115954