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Article

The Enolase of the Haemophilus influenzae Mediates Binding to Collagens: An Extracellular Matrix Component

by
Yesenia Osorio-Aguilar
1,†,
Maria Cristina Gonzalez-Vazquez
2,
Patricia Lozano-Zarain
1,
Ygnacio Martinez-Laguna
1,
Lidia Baylon-Pacheco
3,
Jose Luis Rosales-Encina
3,
Alejandro Carabarin-Lima
2 and
Rosa del Carmen Rocha-Gracia
1,*
1
Posgrado en Microbiología, Laboratorio de Microbiología Hospitalaria y de la Comunidad, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla 72570, Mexico
2
Licenciatura en Biotecnología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla 72570, Mexico
3
Departamento de Infectómica y Patogenesis Molecular, CINVESTAV-IPN, Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, Delegación Gustavo A. Madero, Mexico City 07360, Mexico
*
Author to whom correspondence should be addressed.
†
Current address: Departamento de Farmacología, Facultad de Medicina, Benemérita Universidad Autónoma de Puebla, Puebla 72420, Mexico.
Int. J. Mol. Sci. 2023, 24(21), 15499; https://doi.org/10.3390/ijms242115499
Submission received: 8 August 2023 / Revised: 5 October 2023 / Accepted: 16 October 2023 / Published: 24 October 2023
(This article belongs to the Section Molecular Microbiology)
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Abstract

Enolase proteins play a significant role as moonlighting proteins. In their role as surface-associated enolase, they have multiple functions as they interact with extracellular matrix proteins. Type I and III collagens are the major constituents of this extracellular matrix, and collagen is one of the targets of interaction with the enolase of many pathogens, thereby helping the colonization process and promoting the subsequent invasion of the host. This work aimed to determine the participation of non-typeable H. influenzae enolase as a collagen-binding protein. In this study, through the use of in vitro tests it was demonstrated that recombinant enolase of non-typeable H. influenzae (rNTHiENO) strongly binds to type I collagen. Using molecular docking, the residues that could take part in the interaction of non-typeable H. influenzae enolase-type I collagen (NTHiENO-Cln I) and non-typeable H. influenzae enolase-type III collagen (NTHiENO-Cln III) were identified. However, in vitro assays show that NTHiENO has a better affinity to interact with Cln I, concerning type Cln III. The interaction of NTHiENO with collagen could play a significant role in the colonization process; this would allow H. influenzae to increase its virulence factors and strengthen its pathogenesis.
Keywords: Haemophilus influenzae; enolase; collagen-binding protein Haemophilus influenzae; enolase; collagen-binding protein

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MDPI and ACS Style

Osorio-Aguilar, Y.; Gonzalez-Vazquez, M.C.; Lozano-Zarain, P.; Martinez-Laguna, Y.; Baylon-Pacheco, L.; Rosales-Encina, J.L.; Carabarin-Lima, A.; Rocha-Gracia, R.d.C. The Enolase of the Haemophilus influenzae Mediates Binding to Collagens: An Extracellular Matrix Component. Int. J. Mol. Sci. 2023, 24, 15499. https://doi.org/10.3390/ijms242115499

AMA Style

Osorio-Aguilar Y, Gonzalez-Vazquez MC, Lozano-Zarain P, Martinez-Laguna Y, Baylon-Pacheco L, Rosales-Encina JL, Carabarin-Lima A, Rocha-Gracia RdC. The Enolase of the Haemophilus influenzae Mediates Binding to Collagens: An Extracellular Matrix Component. International Journal of Molecular Sciences. 2023; 24(21):15499. https://doi.org/10.3390/ijms242115499

Chicago/Turabian Style

Osorio-Aguilar, Yesenia, Maria Cristina Gonzalez-Vazquez, Patricia Lozano-Zarain, Ygnacio Martinez-Laguna, Lidia Baylon-Pacheco, Jose Luis Rosales-Encina, Alejandro Carabarin-Lima, and Rosa del Carmen Rocha-Gracia. 2023. "The Enolase of the Haemophilus influenzae Mediates Binding to Collagens: An Extracellular Matrix Component" International Journal of Molecular Sciences 24, no. 21: 15499. https://doi.org/10.3390/ijms242115499

APA Style

Osorio-Aguilar, Y., Gonzalez-Vazquez, M. C., Lozano-Zarain, P., Martinez-Laguna, Y., Baylon-Pacheco, L., Rosales-Encina, J. L., Carabarin-Lima, A., & Rocha-Gracia, R. d. C. (2023). The Enolase of the Haemophilus influenzae Mediates Binding to Collagens: An Extracellular Matrix Component. International Journal of Molecular Sciences, 24(21), 15499. https://doi.org/10.3390/ijms242115499

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