Development of a Mitochondrial Targeting Lipid Nanoparticle Encapsulating Berberine
Abstract
:1. Introduction
2. Results
2.1. Preparation of the R8-MITO-Porter (BBR) Using the Lipid Film Hydration Method
2.2. Evaluation of LNPs and BBR Uptake after Treatment with R8-MITO-Porter (BBR)
2.3. Evaluation of Mitochondrial Respiratory Function after Treatment with R8-MITO-Porter (BBR)
2.4. Evaluation of Intracellular ATP Amounts after Treatment with R8-MITO-Porter (BBR)
2.5. Evaluation of MITOL Expression Level after Treatment with R8-MITO-Porter (BBR)
2.6. Evaluation of Cell Viability after Treatment with R8-MITO-Porter (BBR)
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Preparation of the R8-MITO-Porter (BBR)
4.3. The Method of Removed Unencapsulated BBR
4.4. Cell Cultures and Transfection Study
4.5. Evaluation of Cellular Uptake by a Fluorescence Activated Cell Sorter (FACS)
4.6. Evaluation of the Accumulation of BBR in Cells and Isolated Mitochondria
4.7. Evaluation of Mitochondrial Respiratory Function
4.8. Evaluation of Intracellular ATP Production
4.9. Evaluation of MITOL Gene Expression Level
4.10. Evaluation of Cell Viability
4.11. Statistical Analysis
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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LNP Type | Diameters (nm) | Polydispersity Index (PdI) | ζ-Potential (mV) | Recovery Rate (%) |
---|---|---|---|---|
DOPE/SM-LNP | 102 ± 6.6 | 0.21 ± 0.06 | −5.36 ± 2.4 | |
R8-MITO-Porter | 97.6 ± 5.9 | 0.26 ± 0.02 | 29.5 ± 3.7 | |
R8-MITO-Porter (BBR) | 93.6 ± 1.6 | 0.28 ± 0.01 | 30.1 ± 4.8 | 12.2 ± 4.6 |
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Hori, I.; Harashima, H.; Yamada, Y. Development of a Mitochondrial Targeting Lipid Nanoparticle Encapsulating Berberine. Int. J. Mol. Sci. 2023, 24, 903. https://doi.org/10.3390/ijms24020903
Hori I, Harashima H, Yamada Y. Development of a Mitochondrial Targeting Lipid Nanoparticle Encapsulating Berberine. International Journal of Molecular Sciences. 2023; 24(2):903. https://doi.org/10.3390/ijms24020903
Chicago/Turabian StyleHori, Ikuma, Hideyoshi Harashima, and Yuma Yamada. 2023. "Development of a Mitochondrial Targeting Lipid Nanoparticle Encapsulating Berberine" International Journal of Molecular Sciences 24, no. 2: 903. https://doi.org/10.3390/ijms24020903