Low-Diversity Microbiota in Apical Periodontitis and High Blood Pressure Are Signatures of the Severity of Apical Lesions in Humans
Abstract
:1. Introduction
2. Results
2.1. Description of the General and Clinical Characteristics of the Granuloma and Identification of a Risk Factor “High Blood Pressure”
2.1.1. All Subjects
2.1.2. Epidemiologic Parameters of the Two Groups: PAI ≤ 3 and PAI > 3
2.2. Low Diversity in Granuloma Microbiota Is Associated with the Highest Severity of AP
2.3. Subjects with a Periapical Index PAI > 3 Show a Significant Increase in the Abundance of Propionibacterium, Prevotella7, Pseudomonas and Pseudomonadaceae and a Significant Decrease in the Abundance of the Family Burkholderiaceae
2.4. High Blood Pressure Is an Aggravating Factor for the Severity of Periapical Lesion Associated with a Decreased Diversity of Granuloma Microbiota
3. Discussion
4. Materials and Methods
4.1. Study Design and Settings
4.2. Participants
4.3. Data Collection and Variables
4.4. Microbiota Analysis
4.4.1. Bacterial 16S rRNA Gene Sequencing
4.4.2. 16S rRNA Gene Sequence Analysis
4.4.3. Statistical Analysis
4.5. Data Management and Analysis: Statistical Analyses
4.6. Cohort Low and High Diversity
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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All Subjects (n = 94) | PAI ≤ 3 (n = 44) | PAI > 3 (n = 50) | p Value | |
---|---|---|---|---|
Age (years) | 54.53 ± 14.22 | 55.40 ± 14.48 | 53.76 ± 13.54 | p = 0.36 |
Weight (kg) | 71.39 ± 13.89 | 73.52 ± 15.63 | 69.31 ± 11.99 | p = 0.12 |
Height (cm) | 169.67 ± 13.22 | 171.13 ± 07.92 | 169.35 ± 16.36 | p = 0.4 |
BMI (kg/m2) | 25.66 ± 13.16 | 24.92 ± 4.03 | 23.71 ± 2.84 | p = 0.20 |
Stress Score (Scale 0 to 10) | 4.68 ± 2.68 | 4.71 ± 2.85 | 4.56 ± 2.52 | p = 0.55 |
DMF index (decayed, missing and filled) | 14.63 ± 5.38 | 15.25 ± 5.83 | 14.10 ± 4.95 | p = 0.31 |
Number of decayed teeth (D) | 0.51 ± 1.00 | 0.60 ± 1.10 | 0.42 ± 0.90 | p = 0.36 |
Number of missing teeth (M) | 5.62 ± 3.86 | 6.18 ± 4.37 | 8.96 ± 3.48 | p = 0.18 |
Number of filled teeth (F) | 8.80 ± 4.02 | 8.63 ± 4.60 | 5.14 ± 3.32 | p = 0.63 |
Number of dental brushings/day | 2.01 ± 0.59 | 2.045 ± 0,52 | 1.98 ± 0,65 | p = 0.60 |
PAI score | 3.64 ± 1.09 | 2.63 ± 0.48 | 4.56 ± 0.50 | p < 0.0001 |
HBP− (n = 75) | HBP+ (n = 19) | p Value | |
---|---|---|---|
Age (years) | 53.66 ± 13.96 | 52.5 ± 13.95 | p = 0.77 |
Weight (kg) | 71.49 ± 14.41 | 69.00 ± 06.63 | p = 0.50 |
Height (cm) | 169.09 ± 15.29 | 168.94 ± 5.22 | p = 0.97 |
BMI (kg/m2) | 26.29 ± 15.77 | 24.23 ± 2.62 | p = 0.60 |
Stress Score (scale 0 to 10) | 4.66 ± 2.59 | 4.65 ± 2.65 | p = 0.72 |
DMF index (decayed, missing and filled) | 14.74 ± 5.26 | 13.56 ± 6.03 | p = 0.44 |
Number of decayed teeth (D) | 0.59 ± 1.08 | 0.38 ± 0.89 | p = 0.47 |
Number of missing teeth (M) | 5.62 ± 3.77 | 650 ± 4.83 | p = 3.77 |
Number of filled teeth (F) | 8.75 ± 4.07 | 7.31 ± 3.55 | p = 0.19 |
Number of dental brushings/day | 1.98 ± 0,58 | 2.10 ± 0.65 | p = 0.4437 |
PAI score | 3.47 ± 1.05 | 4.05 ± 0.96 | p = 0.0416 |
Diagnostic of severe periapical diseases (PAI = 5) | 27 % (n = 20) | 42% (n = 8) |
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Minty, M.; Lê, S.; Canceill, T.; Thomas, C.; Azalbert, V.; Loubieres, P.; Sun, J.; Sillam, J.; Terce, F.; Servant, F.; et al. Low-Diversity Microbiota in Apical Periodontitis and High Blood Pressure Are Signatures of the Severity of Apical Lesions in Humans. Int. J. Mol. Sci. 2023, 24, 1589. https://doi.org/10.3390/ijms24021589
Minty M, Lê S, Canceill T, Thomas C, Azalbert V, Loubieres P, Sun J, Sillam J, Terce F, Servant F, et al. Low-Diversity Microbiota in Apical Periodontitis and High Blood Pressure Are Signatures of the Severity of Apical Lesions in Humans. International Journal of Molecular Sciences. 2023; 24(2):1589. https://doi.org/10.3390/ijms24021589
Chicago/Turabian StyleMinty, Matthieu, Sylvie Lê, Thibault Canceill, Charlotte Thomas, Vincent Azalbert, Pascale Loubieres, Jiuwen Sun, Jonathan Sillam, François Terce, Florence Servant, and et al. 2023. "Low-Diversity Microbiota in Apical Periodontitis and High Blood Pressure Are Signatures of the Severity of Apical Lesions in Humans" International Journal of Molecular Sciences 24, no. 2: 1589. https://doi.org/10.3390/ijms24021589