Fabrication and Performance Evaluation of Gelatin/Sodium Alginate Hydrogel-Based Macrophage and MSC Cell-Encapsulated Paracrine System with Potential Application in Wound Healing

Yao, Hang; Yuan, Xiaohui; Wu, Zhonglian; Park, Sumin; Zhang, Wang; Chong, Hui; Lin, Liwei; Piao, Yuanzhe

doi:10.3390/ijms24021240

Open AccessArticle

Fabrication and Performance Evaluation of Gelatin/Sodium Alginate Hydrogel-Based Macrophage and MSC Cell-Encapsulated Paracrine System with Potential Application in Wound Healing

by

Hang Yao

^1,†

,

Xiaohui Yuan

^1,†,

Zhonglian Wu

¹,

Sumin Park

²,

Wang Zhang

^1,2,3

,

Hui Chong

^1,*,

Liwei Lin

^2,*

and

Yuanzhe Piao

^2,3

¹

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225009, China

²

Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea

³

Advanced Institutes of Convergence Technology, Suwon 16229, Republic of Korea

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2023, 24(2), 1240; https://doi.org/10.3390/ijms24021240

Submission received: 27 December 2022 / Revised: 5 January 2023 / Accepted: 5 January 2023 / Published: 8 January 2023

(This article belongs to the Special Issue Novel Natural Compound for Wound and Tissue Repair and Regeneration)

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Abstract

A gelatin/sodium alginate-based hydrogel microsphere has been fabricated after reaction condition optimization. Macrophages (RAW246.7) and adipose mesenchymal stem cells (ADSC) have been subsequently encapsulated in the microsphere in order to construct a 3D paracrine system for wound healing treatment. The synthesized microsphere displayed neglectable cytotoxicity toward both encapsulated cells until 10 days of incubation, indicating promising biocompatibility of the microsphere. A qRT-PCR and ELISA experiment revealed positive regulation of cytokines (Arg-1, IL-6, IL-8, IL-10, bFGF, HGF, VEGF, TLR-1, and CXCL13) expression regarding macrophage phenotype transformation and anti-inflammatory performance both inside the microsphere and in the microenvironment of established in vitro inflammatory model. Additionally, positive tendency of cytokine expression benefit wound healing was more pronounced in a fabricated 3D paracrine system than that of a 2D paracrine system. Furthermore, the 3D paracrine system exhibited more efficiently in the wound healing rate compared to the 2D paracrine system in an in vitro model. These results suggested the current paracrine system could be potentially used as a robust wound healing dressing.

Keywords: wound healing dressing; paracrine system; biocompatibility; 3D cell culture; natural polymer

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MDPI and ACS Style

Yao, H.; Yuan, X.; Wu, Z.; Park, S.; Zhang, W.; Chong, H.; Lin, L.; Piao, Y. Fabrication and Performance Evaluation of Gelatin/Sodium Alginate Hydrogel-Based Macrophage and MSC Cell-Encapsulated Paracrine System with Potential Application in Wound Healing. Int. J. Mol. Sci. 2023, 24, 1240. https://doi.org/10.3390/ijms24021240

AMA Style

Yao H, Yuan X, Wu Z, Park S, Zhang W, Chong H, Lin L, Piao Y. Fabrication and Performance Evaluation of Gelatin/Sodium Alginate Hydrogel-Based Macrophage and MSC Cell-Encapsulated Paracrine System with Potential Application in Wound Healing. International Journal of Molecular Sciences. 2023; 24(2):1240. https://doi.org/10.3390/ijms24021240

Chicago/Turabian Style

Yao, Hang, Xiaohui Yuan, Zhonglian Wu, Sumin Park, Wang Zhang, Hui Chong, Liwei Lin, and Yuanzhe Piao. 2023. "Fabrication and Performance Evaluation of Gelatin/Sodium Alginate Hydrogel-Based Macrophage and MSC Cell-Encapsulated Paracrine System with Potential Application in Wound Healing" International Journal of Molecular Sciences 24, no. 2: 1240. https://doi.org/10.3390/ijms24021240

APA Style

Yao, H., Yuan, X., Wu, Z., Park, S., Zhang, W., Chong, H., Lin, L., & Piao, Y. (2023). Fabrication and Performance Evaluation of Gelatin/Sodium Alginate Hydrogel-Based Macrophage and MSC Cell-Encapsulated Paracrine System with Potential Application in Wound Healing. International Journal of Molecular Sciences, 24(2), 1240. https://doi.org/10.3390/ijms24021240

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Fabrication and Performance Evaluation of Gelatin/Sodium Alginate Hydrogel-Based Macrophage and MSC Cell-Encapsulated Paracrine System with Potential Application in Wound Healing

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