Predicting Modifiers of Genotype-Phenotype Correlations in Craniofacial Development
Abstract
:1. Introduction
2. Results
2.1. Targets of Gata3 in Cranial Neural Crest Cells
2.2. Transcriptomic Data Can Predict Chemical Modifiers of Genotype-Phenotype Correlations
3. Discussion
3.1. Prediction of Chemicals That Worsen Mutant Phenotypes
3.2. Identification of Chemicals That Are Protective against Deleterious Phenotypes
3.3. Identification of Functional Modules through RNA-seq
4. Materials and Methods
4.1. Animal Care and Use
4.2. Injections and Heat Shock
4.3. Sample Preparation and FACS
4.4. cDNA Library Preparation and RNA Sequencing
4.5. Differential Expression Analysis
4.6. Identification and Testing of Chemical Modifiers
4.7. Quantitative Reverse Transcription PCR (RT-qPCR)
4.8. Trabecular Measurements
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Predicted Modification | Chemical | Function | CAS Registry Number |
---|---|---|---|
Phenotype enhancer | Flubendazole | Microtubule inhibitor, acetylcholinesterase inhibitor | 31430-15-6 |
Rigosertib | Cell cycle inhibitor, PLK inhibitor | 1225497-78-8 | |
Etacrynic acid | Na/K/Cl transporter | 58-54-8 | |
Clofibric acid | PPAR receptor agonist | 882-09-7 | |
Nocodazole | Tubulin inhibitor, tubulin polymerization inhibitor | 31430-18-9 | |
Vinorelbine | Tubulin inhibitor, apoptosis stimulant | 71486-22-1 | |
Vinblastine | Microtubule inhibitor, tubulin inhibitor | 865-21-4 | |
Vincristine | Microtubule inhibitor, tubulin inhibitor | 57-22-7 | |
Phenotype suppressor | PD-184352 | MEK inhibitor, MAP kinase | 212631-79-3 |
Triptolide | RNA polymerase inhibitor | 38748-32-2 | |
Belinostat | HDAC inhibitor, cell cycle inhibitor | 866323-14-0 | |
BMS-191011 | Calcium activated potassium channel opener, potassium channel activator | 202821-81-6 | |
ISOX | HDAC inhibitor, cell cycle inhibitor | 1045792-66-2 | |
Daunorubicin | RNA synthesis inhibitor | 20830-81-3 | |
Chromanol | Potassium channel blocker | 1481-93-2 | |
Alvocidib | CDK inhibitor, apoptosis stimulant | 146426-40-6 |
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Kar, R.D.; Eberhart, J.K. Predicting Modifiers of Genotype-Phenotype Correlations in Craniofacial Development. Int. J. Mol. Sci. 2023, 24, 1222. https://doi.org/10.3390/ijms24021222
Kar RD, Eberhart JK. Predicting Modifiers of Genotype-Phenotype Correlations in Craniofacial Development. International Journal of Molecular Sciences. 2023; 24(2):1222. https://doi.org/10.3390/ijms24021222
Chicago/Turabian StyleKar, Ranjeet D., and Johann K. Eberhart. 2023. "Predicting Modifiers of Genotype-Phenotype Correlations in Craniofacial Development" International Journal of Molecular Sciences 24, no. 2: 1222. https://doi.org/10.3390/ijms24021222