Transcriptional Regulators Controlling Virulence in Pseudomonas aeruginosa
Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín-Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(15), 11895; https://doi.org/10.3390/ijms241511895
Submission received: 4 July 2023
/
Revised: 21 July 2023
/
Accepted: 22 July 2023
/
Published: 25 July 2023
(This article belongs to the Special Issue Molecular Mechanisms of Transcriptional Regulation in Bacteria)
Abstract
:Pseudomonas aeruginosa is a pathogen capable of colonizing virtually every human tissue. The host colonization competence and versatility of this pathogen are powered by a wide array of virulence factors necessary in different steps of the infection process. This includes factors involved in bacterial motility and attachment, biofilm formation, the production and secretion of extracellular invasive enzymes and exotoxins, the production of toxic secondary metabolites, and the acquisition of iron. Expression of these virulence factors during infection is tightly regulated, which allows their production only when they are needed. This process optimizes host colonization and virulence. In this work, we review the intricate network of transcriptional regulators that control the expression of virulence factors in P. aeruginosa, including one- and two-component systems and σ factors. Because inhibition of virulence holds promise as a target for new antimicrobials, blocking the regulators that trigger the production of virulence determinants in P. aeruginosa is a promising strategy to fight this clinically relevant pathogen.
1. Introduction
Pseudomonas aeruginosa is clinically recognized for being an opportunistic pathogen of humans that causes severe infections, mainly respiratory tract infections but also urinary tract, ear, and eye infections, and bacteraemia and sepsis [1]. As an opportunistic pathogen, P. aeruginosa is rarely pathogenic in healthy individuals, and the development of infections usually depends on epithelial injuries or impaired immune defenses [2]. Nevertheless, P. aeruginosa is a primary source of hospital-acquired infections especially in mechanically ventilated patients in intensive care unit, and burns, open wounds, and post-surgery patients [3]. Moreover, it is the main reason for lung function deterioration and mortality in cystic fibrosis (CF) patients, who are chronically infected with this pathogen [4]. Infections caused by P. aeruginosa are often life-threatening, and treatment can be challenging because this bacterium is intrinsically resistant to multiple antibiotics and can easily acquire new resistances [5]. In fact, antibiotic resistance among P. aeruginosa has escalated globally over the past three decades, and several outbreaks in hospitals have highlighted the need for controlling multi-drug resistant P. aeruginosa infection and spread [6]. For this reason, the World Health Organization (WHO) declared this bacterium a priority pathogen, demanding research and the development of new strategies for its eradication.
P. aeruginosa owes its pathogenic versatility to a large arsenal of cell-associated and secreted virulence factors that enable host colonization and protection from host innate immune defenses. The main virulence factors and hallmark capabilities associated with virulence produced by this pathogen include (1) bacterial motility and attachment factors; (2) biofilm formation factors; (3) extracellular invasive enzymes and secreted toxins; (4) toxic secondary metabolites; and (5) iron acquisition systems and factors controlling iron homeostasis [7] (Table 1).
1.1. Motility and Attachment Factors
P. aeruginosa motility depends on the flagellum and the type IV pili. These appendages control the motility of the bacterium (Table 1) and are involved in important virulence processes including searching for a desirable surface and allowing cell-surface contact, transitioning from a planktonic to a sessile lifestyle, and promoting biofilm formation [8]. Flagella are polar protein complexes that use hydrodynamic forces to propel the bacterial cell in liquid (swimming motility) and semi-solid surfaces (swarming motility) [8]. There are two main subfamilies of type IV pili, namely, type IVa and type IVb. Twitching motility on solid surfaces is driven by type IVa pili, and they cooperate with flagella to perform swarming motility. Type IVa pili are composed of a large number of PilA pilin subunits and are retractile thanks to the action of PilT ATPase [9]. Type IVb pili, which are also known as tight adherence (Tad) pili, are composed of Flp pilin subunits. Tad pili are non-retractile, so they are mainly involved in the attachment of P. aeruginosa to abiotic and biological surfaces, a critical step for proper colonization and infection of host tissues and for biofilm formation [9,10]. The major adhesins, known as chaperone-usher pathway (CUP) pili, are also important for P. aeruginosa attachment to host surfaces. This bacterium produces five different CUP systems (from CupA to CupE) (Table 1) [11]. Other proteins important for P. aeruginosa attachment to solid surfaces and adhesion to host cells are the biofilm associated protein BapA and lectins LecA and LecB (Table 1) [12,13].
1.2. Biofilm Formation Factors
The ability of P. aeruginosa to form a biofilm is key for the establishment of chronic infections. Although it is expected that other species present in the host participate in the formation of biofilms with P. aeruginosa, most experimental data on virulence factors and their regulation were obtained using analyses of biofilms from P. aeruginosa monocultures. The main constituents of P. aeruginosa biofilms are exopolysaccharides (EPSs), i.e., alginate, Pel, and Psl, and extracellular DNA (eDNA) [14] (Table 1). Alginate is considered an important virulence factor of P. aeruginosa because overproduction of this EPS results in the clinically relevant mucoid phenotype [15]. Mucoid cells grow as compact microcolonies, which confer protection against antibiotics and immune system attacks, thus contributing to the long-term persistence of P. aeruginosa in chronic infections [15]. This phenotype also leads to the establishment of concomitant airway inflammation and is the main cause of mortality in CF patients [15]. Rhamnolipids, which are extracellular secondary metabolites, also participate in different stages of P. aeruginosa biofilm development, from the earliest cell-to-surface interactions to the maintenance and dispersion of the biofilm architecture [16] (Table 1). Rhamnolipids are considered important virulence factors because they mediate the active dispersal of cells from biofilms, thus promoting the colonization of new niches, and act in biofilms as protective agents against phagocytes [17]. Moreover, the amphiphilic properties of rhamnolipids make them capable of disrupting host cell tight junctions when being incorporated into host membranes [18]. In addition to rhamnolipids, P. aeruginosa produces glycine betaine, another bacterial surfactant that confers cells resistance to osmotic stress, protects them from phagocytosis, and increases the survival of P. aeruginosa in the mouse lung during infection [19].
Table 1.
Transcriptional regulators of the main P. aeruginosa virulence hallmarks *.
| Virulence Hallmark | Virulence Factors | Genes Involved | Function in Virulence | Main Transcriptional Regulator(s) | References |
|---|---|---|---|---|---|
| Bacterial motility and attachment | Flagellum | flg, fli, and flh gene clusters | Swimming and swarming motility | AmrZ, PilS-PilR, FleS-FleR, σFliA | [20,21,22] |
| Type IV pili | flp, tad, afimU fimV, and pilABCD | Twitching motility and attachment to solid surfaces | AmrZ, PchR, PilS-PilR, PprA-PprB, GacS-GacA, BfiS-BfiR, GtrS-GltR, σSigX | [12,22,23,24,25,26,27,28] | |
| Adhesins | bapA, cupA, cupB, cupC, cupD, and cupE gene clusters | Attachment to host cells and solid surfaces | RocS-RocR-RocA1, RcsC-RcsB, PvrS-PvrR, PprA-PprB, GacS-GacA, BfiS-BfiR, GtrS-GltR, σSigX, σRpoS | [11,23,24,26,27,29,30,31] | |
| Lectins | lecA and lecB | Adherence to epithelial cells | PqsR, Fur, PchR, σAlgT | [28,32,33,34] | |
| Biofilm formation | Esopolysaccharide alginate | alg gene cluster | Component of the extracellular matrix | AmrZ, PchR, FimS-AlgR, KinB-AlgB, σAlgT, σRpoH, σRpoS, σSbrI | [28,35,36,37,38,39,40] |
| Exopolysaccharides Pel and Psl | pel and psl gene clusters | Component of the extracellular matrix | GacS-GacA, BfiS-BfiR, GtrS-GltR, σAlgT, σRpoS | [24,26,27,33,36,37,41] | |
| eDNA | phdA | Component of the extracellular matrix | BfmS-BfmR | [42] | |
| Rhamnolipids | rhlAB and rhlC | Biosurfactant | RhlR, PtxR | [43,44] | |
| Glycine betaine | betA and betB | Biosurfactant | BetI, GbdR | [45] | |
| Extracellular invasive enzymes and toxins | Elastases | lasA and lasB | Degradation of host elastin and collagen | LasR, RhlR, PtxR, PchR, FleS-FleR, σAlgT | [28,43,44,46,47,48,49] |
| Protease IV | piv/prpL | Degradation of host fibrinogen | Fur, σPvdS, σFpvI | [50,51] | |
| Alkaline protease | aprA | Degradation of host transferrin and matrix-associated proteins | LasR, PvrA, σSigX, σAlgT | [23,46,47,52] | |
| Phospholipases | plcH, exoU, pldA, pldB, and tplE | Degradation of host cell membranes and lung surfactants | ExsA, GbdR, PvrA, SphR, GacS-GacA BfiS-BfiR, GtrS-GltR, σFliA | [45,52,53,54] | |
| Exotoxin S and T | exoS and exoT | Cytotoxicity and cytoskeleton disruption | ExsA, GacS-GacA, BfiS-BfiR, GtrS-GltR, σFliA | [24,26,27,53,55] | |
| ExoY adenylate cyclase | exoY | Disruption of actin cytoskeleton and increased host cell permeability | ExsA, GacS-GacA, BfiS-BfiR, GtrS-GltR, σFliA | [24,26,27,53,55] | |
| Exotoxin A | exoA/toxA | Inhibition of host protein synthesis | PtxR, PtxS, GtrS-GltR, FleS-FleR, σPvdS | [48,51,56,57] | |
| Pro-inflammatory toxins | lptA, lptE, and osmE | Induction of host inflammatory response | σAlgT | [58] | |
| Toxic secondary metabolites | Hydrogen cyanide | hcnABC | Arrest respiration in host cells | LasR, RhlR, PchR, AmpR | [28,47,59] |
| Phenazine and pyocyanin | phzABCDEFG phzM | Cause oxidative stress and cytotoxicity | RhlR, PqsR, CdpR, PvrA, PchR, AmpR | [28,43,44,52,59,60] | |
| Acquisition and homeostasis of iron | Pyoverdine | pvd gene cluster | Siderophore production and iron acquisition | Fur, AmpR, σPvdS, σFpvI | [59,61,62] |
| Pyochelin | pchDCBA, pchEF, and fptABCX gene clusters | Siderophore production, iron acquisition, and tissue damage | Fur, PchR | [61] | |
| Heme and hemophore | phu, has, and hxu gene clusters | Iron acquisition from heme | Fur, AmpR, σHasI, σHxuI | [59,61,62] | |
| Iron storage and detoxification | prrF1, prrF2, brfB, sodB, and katA genes | Prevent iron accumulation and toxicity | Fur, PchR | [28,61] |
* Adapted from [7].
1.3. Extracellular Invasive Enzymes and Toxins
Extracellular invasive enzymes that degrade a variety of host connective tissues and immune components (Table 1) are essential in the first steps of host tissue colonization by P. aeruginosa. Particularly important to the pathogenicity of this bacterium are extracellular proteases, including elastase B (LasB, pseudolysin), elastase A (LasA, staphylolysin), protease IV (PIV, also known as lysyl endopeptidase PrpL), and alkaline protease (AprA, aeruginolysin) (reviewed in [63]). LasB has elastinolytic activity toward human elastin and also degrades collagens and several components of innate and adaptive immune defenses like tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-2 (IL-2), and the surfactant proteins A and D (SP-A and SP-D) required for phagocytosis of pathogens. Phagocytic evasion is also enhanced by the alkaline protease AprA, which inhibits chemotaxis of neutrophils and degrades complement proteins and cytokines (i.e., IFN-α, TNF-γ, and IL-6). The PIV/PrpL protease cleaves fibrinogen, thus playing a role in tissue damage and invasion by P. aeruginosa. This protease can also degrade plasminogen, immunoglobulin, complement proteins, and host antimicrobial peptides. The LasA, LasB, and PIV/PrpL proteases are secreted to the extracellular medium by the P. aeruginosa Xcp type II secretion system (Xcp-T2SS), while AprA is secreted by the type I secretion system (T1SS) [63].
Other important extracellular invasive enzymes produced by P. aeruginosa are phospholipases, i.e., lipolytic esterases used by the pathogen to hydrolyze eukaryotic phospholipids (among other functions) (Table 1). This includes the Xcp-T2SS-secreted phospholipase C PlcH, which can hydrolyze several different phospholipids with preference for choline containing phospholipids (i.e., sphingomyelin and phosphatidylcholine) [64]. Hydrolysis of sphingomyelin and phosphatidylcholine yields phosphocholine and ceramide and diacylglycerol, respectively, which are involved in important signal transduction cascades in mammalian cells (i.e., growth, differentiation, apoptosis, proliferation, inflammation). Moreover, PlcH has hemolytic activity and causes hemolysis of human and erythrocytes. This activity is enhanced by the ceramidase CerN encoded within the plcH gene cluster [65]. Thus, PlcH promotes P. aeruginosa dispersion during infection by providing host-derived nutrients (i.e., choline and iron from erythrocytes) and by triggering signaling pathways that lead to inflammation. Importantly, PlcH is highly lethal to endothelial cells and inhibits angiogenesis (i.e., formation of new blood vessels) [66].
A major pathogenicity strategy of P. aeruginosa is the production of enzymes and toxins that induce cytotoxicity and various forms of cell death in target host cells [67]. ExoU (for extotoxin U) is a potent cytotoxic A2 phospholipase capable of causing rapid cell death in eukaryotic cells by producing a loss in plasma membrane integrity and necrosis [68] (Table 1). The production of ExoU was found to correlate with acute lung injury and sepsis in an animal model of pneumonia and in immunocompromised patients [69,70]. ExoU killing seems to be directed against phagocytes and epithelial barriers, thus promoting bacterial persistence and dissemination. ExoU is secreted via the type III secretion system (T3SS), a needle complex also designated as the injectosome used by P. aeruginosa to inject toxic effectors directly into the cytosol of host cells [68]. Other T3SS-secreted toxins include ExoS, ExoT, and ExoY [68] (Table 1). ExoS and ExoT are bifunctional proteins consisting of an N-terminal GTPase-activating protein (GAP) domain and a C-terminal ADP-ribosyltransferase (ADPRT) domain. The GAP domain in both toxins targets small GTPases required by eukaryotic cells to coordinate and maintain the actin cytoskeleton [68]. The ADPRT domain of ExoS targets many host proteins, thereby producing a number of adverse effects on host cells (i.e., actin cytoskeletal disruption associated with cell rounding, inhibition of DNA synthesis, vesicular trafficking and endocytosis, and cell death) [68]. The ExoT ADPRT domain targets a distinct and more limited number of host proteins, and its activity results in the inhibition of cell migration, adhesion, and proliferation, thus blocking phagocytosis and disrupting epithelial barriers that facilitate bacterial dissemination. Although the functions of ExoS have been thought for a long time to be only cytotoxic and phagocytic, it was recently shown that this toxin also promotes the internalization of P. aeruginosa into eukaryotic cells and protection of the intracellular niche [71]. The last T3SS-secreted toxin with a known role during infection and host colonization is ExoY, an adenylate cyclase that increases the intracellular cAMP concentration in eukaryotic cells, producing the differential expression of multiple cAMP-regulated genes [68] (Table 1). The function of this enzyme leads to a disruption in the actin cytoskeleton, the inhibition of bacterial uptake by host cells, and increased endothelial permeability.
A key determinant of P. aeruginosa’s pathogenicity is the exotoxin (or toxin) A (ExoA or ToxA), a potent toxin secreted by Xcp-T2SS. ToxA contains four distinct structural and functional domains responsible for cell recognition, translocation of the toxin across cellular membranes, and the catalytic ADP-ribosyltransferase activity of this toxin [72]. Once internalized in host cells, ToxA catalyzes the ADP-ribosylation of the elongation factor-2 (eEF-2), resulting in the inhibition of protein synthesis and causing apoptotic cell death [73].
P. aeruginosa produces exoenzymes and exotoxins that are injected into eukaryotic cells via other secretion systems such as the type VI secretion system (T6SS). Particularly, the phospholipase D enzymes PldA and PldB, secreted by H2- and H3-T6SS, respectively, target the host PI3K (phosphoinositide 3-kinase)/Akt pathway allowing internalization of P. aeruginosa into epithelial cells [74,75]. This process is enhanced by the secretion of VgrG2b via H2-T6SS. VgrG2b targets microtubule components, including the γ-tubulin ring complex (γTuRC) that catalyzes microtubule nucleation, promoting a microtubule-dependent internalization of P. aeruginosa [76]. H2-T6SS is also used by P. aeruginosa to inject the Tle4 phospholipase family protein TplE into eukaryotic cells. TplE contains a eukaryotic PGAP1 (post-glycosylphosphatidylinositol attachment to proteins 1)-like domain that allows targeting the host’s endoplasmic reticulum (ER), promoting ER stress and autophagy [77].
Furthermore, some lipoproteins produced by P. aeruginosa can act as pro-inflammatory lipotoxins, causing an excessive inflammatory response in the lungs of CF patients (Table 1). This includes lipopolysaccharide transport proteins A and B (LptA and LptB, respectively) and the osmoprotective lipoprotein OsmE, which increase the production of interleukin-8 in host epithelial tissues and human macrophages [58].
1.4. Toxic Secondary Metabolites
P. aeruginosa also produces toxic secondary metabolites that induce host cell cytotoxicity (Table 1). This pathogen is notably known for the production of phenazines, which are redox-active compounds that play a major role in antibiotic resistance and virulence. Of special relevance is pyocyanin, a phenazine-derived pigment capable of inducing apoptosis in neutrophils by generating reactive oxygen species (ROS) that damage mitochondria [78]. This metabolite is crucial for P. aeruginosa to establish a chronic infection and to induce lung damage in mice [79]. P. aeruginosa also produces hydrogen cyanide, especially under low oxygen conditions and upon reaching high cell density. This toxic metabolite disrupts aerobic respiration of the host cells by binding to the Fe3+ of the cytochrome oxidase in the respiratory chain [80]. Additionally, its structural similarity with oxygen allows cyanide to bind to other metalloproteins, impairing a wide range of cell functions [80].
1.5. Iron Acquisition Systems and Factors Controlling Iron Homeostasis
Iron is an essential nutrient for all living organisms because it works as a redox cofactor of enzymes required for several vital processes. In the human body, iron is mainly found complexed with heme in hemoproteins or sequestered in the iron-storage protein ferritin inside the cell [61]. During infection, the host limits the availability of iron by inducing iron sequestration in order to combat invading microorganisms, a phenomenon known as ‘nutritional immunity’. To achieve this, the host increases the production of iron-scavenging molecules, haptoglobin, and hemopexin, which chelates free heme or hemoglobin [61]. To fulfil their iron requirements in the host, P. aeruginosa produces pyoverdine and pyochelin siderophores and the HasAp hemophore, which are high-affinity iron- and heme-chelating compounds, respectively [61] (Table 1). Pathogens have developed a preference for heme as iron source during the early stages of infection. Accordingly, P. aeruginosa possesses three different systems for heme acquisition: Phu, Has, and Hxu [81]. The TonB-dependent transporters (TBDTs) PhuR and HasR undertake transport into the periplasm of free heme and heme-HasAp complexes, respectively. The TBDT HxuA, however, plays a smaller role in heme acquisition and seems to be instead involved in the activation of virulence upon sensing heme from the host [61,81]. Pyoverdine has an extremely high affinity for iron and is even capable of stealing iron from the host’s iron-scavenging proteins, such as lactoferrin and transferrin, which confers a huge advantage to the pathogen during infection [61]. Iron competition during the host–pathogen interaction is a crucial process that can determine the success of an infection [61]. Accordingly, a pyoverdine-defective mutant P. aeruginosa strain shows attenuated virulence in mice and Caenorhabditis elegans [82,83]. Pyochelin has a lower affinity for iron than pyoverdine, but its synthesis is less energetically burdening and thus preferred when iron is moderately limited [7]. Pyochelin is known to work together with pyocyanin to induce oxidative stress and inflammation in host cells and to cause tissue damage during chronic infections by provoking a sustained inflammatory response [7].
Controlling iron homeostasis is also critical to survive within the host during infection. High amounts of intracellular iron lead to the production of reactive oxygen species (ROS) via Fenton reactions, eventually resulting in cell wall and DNA damage and lipid peroxidation [84]. In order to survive iron toxicity, bacteria have developed mechanisms to store excess of iron in heteropolymeric complexes known as bacterioferritins. In P. aeruginosa, bacterioferritins are composed of BfrA and BfrB subunits arranged in a spherical shell, which captures and reduces the levels of free iron in the cytoplasm [61]. P. aeruginosa also has a means to defend itself from oxidative damage caused by excess iron, such as superoxide dismutase SodB and catalase KatA, which target superoxide anions and hydrogen peroxide under high iron levels, respectively [61]. The response to iron toxicity is further modulated by the two small RNAs (sRNAs) PrrF1 and PrrF2, which block the production of iron storage proteins, non-essential iron-containing proteins, oxidative stress resistance, and quinolones [61,85]. These sRNAs are particularly expressed during P. aeruginosa infection in CF patients and were found to be required for proper infection in a murine model of lung infection [61,85].
1.6. Regulation of Virulence
Importantly, all these P. aeruginosa virulence factors are under tight regulation to prevent their production when they are not required. This is particularly important when colonizing a host to avoid alarming the immune system during the first stages of infection. Gene expression in bacteria is primarily regulated at the level of transcription initiation by modifying the affinity of RNA polymerase (RNAP) for DNA. This occurs through two main mechanisms, namely, with the substitution of the σ subunit in RNAP and with the action of transcription factors (TFs) [86]. The σ factor in RNAP is a dissociable subunit required for promoter recognition and transcription initiation. P. aeruginosa contains two families of σ factors, the σ70 and σ54 families, which are structurally different and use a different mechanism for transcription initiation [20]. While σ70 factors can perform this process on their own, σ54 factors require the function of ATP-dependent enhancer binding proteins (EBPs). EBPs normally consist of an N-terminal sensor domain, a conserved central AAA+ ATPase domain, and a C-terminal DNA binding domain [87]. Upon sensing the inducing stimulus, EBPs bind to the enhancer-binding site on target genes located upstream of the σ54 binding site and energize transcription initiation from σ54 promoters via their AAA+ ATPase domains. [87]. The σ70 family has been classified in four major phylogenetic groups based on structure and function, with Group 1 consisting of primary σ factors that triggers transcription of essential general functions and Groups 2–4 consisting of alternative σ factors (reviewed in [88,89]). The activity of RNAP loaded with primary or alternative σ factors can be modulated by TFs. TFs are DNA-binding proteins that bind to DNA regions known as operators, thereby blocking or promoting transcription initiation (reviewed in [90]). TFs control gene transcription in response to intra- or extracellular signals, and their activity is often modulated by the signal itself. Most TFs have a sensing or input domain for signal binding and an output DNA-binding domain that produces a response to the signal by modifying gene transcription. These are known as one-component systems (OCSs) and are especially abundant in bacteria that are capable of colonizing many different environments [91] like P. aeruginosa. TFs can be associated with sensor histidine kinases, forming the so-called two-component systems (TCSs), which are extensively present in P. aeruginosa [92]. Virulence in this pathogen is also controlled by quorum sensing (QS) in response to cell density, a mechanism that involves the activation of OCS proteins. This review highlights the OCSs, TCSs, and σ factors of P. aeruginosa that regulate the production of virulence factors during infection.
2. Control of P. aeruginosa Virulence by One-Component Systems
One-component systems (OCSs) are the simplest and most responsive transcriptional regulators of bacteria. Canonical OCSs are composed of a single cytoplasmic protein that has an input or sensor domain for signal recognition and an output DNA-binding domain that responds to the signal by modifying gene transcription (Figure 1a) [91]. One-component proteins have a wide diversity of input domains that allow the detection of multiple different signals. For the output domain, around 84% of one-component proteins have a helix-turn-helix (HTH) domain, which binds to DNA and activates or represses gene transcription [91]. However, a minority of these proteins have another type of output domain including enzymatic domains that regulate cyclic nucleotides production and protein phosphorylation. The cytosolic location of one-component proteins reduces the type of external signals that can access this bacterial compartment to be recognized by the sensor domain. For this reason, one-component systems recognize signals produced in the cytosol or small molecules able to diffuse through the membrane, like light or gases [91]. Upon signal recognition, the output domain undergoes a conformational change that induces a response, usually by exposing the HTH domain of the protein and allowing its binding to DNA [93]. The presence of a sensor and effector domain in a single protein allows for a quick response to the signal in detriment to the fine-tuned and more precise response carried out by transcriptional regulators, in which these functions reside in different proteins (e.g., two-component systems). P. aeruginosa harbors between 408 and 452 OCS proteins, with the type strain PAO1 encoding 423 regulators of this type [92].
2.1. OCSs Responding to Quorum Sensing (QS)
QS responds to cell population density, enabling bacteria to restrict the expression of specific genes to high cell densities at which the resulting phenotypes will be most beneficial. In P. aeruginosa, QS is performed by three autoinducers, two N-acyl homoserine lactones (AHLs), and a quinolone signal-designed PQS (from Pseudomonas quinolone signal) [94]. These molecules are produced by P. aeruginosa and diffuse freely through the membrane into the extracellular medium. When the cell density is high and the concentration of the autoinducer is above the threshold, it is recognized by a OCS protein that responds by modifying gene expression [94].
2.1.1. LasR
LasR belongs to the LuxR family of regulators, which typically acts as transcriptional activators, and is the main regulator of the Las QS system in P. aeruginosa. LasR senses the autoinducer N-(3-oxododecanoyl)homoserine lactone (3O-C12-HSL), a product of the lasI gene, and functions as a global regulator that directly or indirectly activates the expression of over 300 genes, many of which are involved in virulence [47] (Figure 2). In addition to lasI, these include the elastase genes lasA and lasB, the hcnABC cluster for the production of hydrogen cyanide, plcB phospholipase, the flp-type IVb pili subunit, aprA alkaline protease, the pyoverdine regulator pvdS, and the toxA exotoxin genes [47,95] (Table 1). LasR also induces the expression of the RsaL repressor, which dampens the expression of LasR targets [96]. LasR occupies the highest position in the hierarchical QS signaling network, playing a key role activating the transcription of the Rhl and Pqs QS systems (Figure 2) [44]. Importantly, P. aeruginosa lasR and lasI mutants are considerably less virulent than the wild-type strain in a burn mouse model of infection and a rat model of acute pneumonia, and the lack of LasR produces a lower inflammatory response and impaired host colonization [44,49,97].
2.1.2. RhlR
Mirroring LasR, the LuxR family regulator RhlR is the transcriptional activator of the Rhl QS system, which detects the N-butanoyl-L-homoserine lactone (C4-HSL) signal molecule generated by the product of the rhlI gene [98]. RhlR directly or indirectly activates the expression of over 100 genes, including several virulence genes like rhlA and rhlB for synthesis of rhamnolipids, and LasR targets lasB, hcnABC, and phenazine genes [44] (Figure 2). P. aeruginosa rhlR and rhlI mutants are considerably less virulent than the wild-type strain in murine infection models [99,100]. Despite the similarities and overlapping functions between the Las and Rhl systems, each one contributes to virulence independently, as evidenced by the synergistic effects on virulence observed when both systems are mutated [95,101]. A double lasR rhlR mutant resulted in lower mortality rates for the nematode C. elegans than single lasR and rhlR mutants [101]. Similarly, a lasI rhlI double mutant was more lethal than single mutants in a burn mouse model of infection [95]. Surprisingly, whilea lasR or rhlR single mutation reduces the cytotoxicity of P. aeruginosa towards lung epithelial cells, the cytotoxicity of the lasR rhlR double mutant is higher than that of the wild-type strain [101]. This suggests that some elements of the LasR and RhlR regulons may act in opposite directions [101].
2.1.3. PqsR
The third QS regulator of P. aeruginosa is PqsR (also named MvfR), a transcriptional regulator of the LysR family that senses PQS, a quinole synthesized by the pqsABCDE and phnAB operons and the pqsH gene [34]. PqsR upregulates the expression of more than 100 genes, including the pqsABCDE and phnAB operons, and many genes of the RhlR regulon [34] (Figure 2). This overlap between these two QS systems seems to arise from PqsE thioesterase, which is not only required for PQS synthesis but also for RhlR activity [34]. Among others, PqsR activates the expression of genes encoding the virulence factors pyocyanin and hydrogen cyanide and the lectin LecA [34] (Table 1). A pqsR mutation reduces the mortality rate caused by P. aeruginosa in a burn mouse model of infection, but this effect is likely due to reduced expression of the pqsE gene, and therefore to the reduced RhlR activity in this mutant [34].
Moreover, PQS regulates the expression of many genes involved in iron acquisition such as pyoverdine and pyochelin biosynthetic genes [102]. This is likely an indirect effect due to the iron-chelating properties of PQS [61,102]. Interestingly, PqsR not only responds to PQS but also to its precursor molecule HHQ [44]. The pqsH gene encoding the enzyme required for the conversion of HHQ into PQS is the only gene in the biosynthetic cluster that is not regulated by PqsR but by the AraC family regulator CdpR [44,60]. According to the repressor role of this regulator, a P. aeruginosa cdpR mutant produced a higher mortality rate and increased lung injury and inflammation in a mouse model of acute pneumonia [60].
2.2. OCSs Regulating Motility, Attachment, and Biofilm Formation
AmrZ
The P. aeruginosa AmrZ (from alginate and motility regulator) belongs to the ribbon-helix-helix family of transcriptional regulators, which mainly functions as repressors [103]. While acting as a repressor for nearly 50 genes, AmrZ can also act as an activator, controlling the expression of virulence genes such as P. aeruginosa alginate synthesis and transport genes encoded within the alg cluster [39,104] (Figure 3). Because alginate production promotes P. aeruginosa biofilm formation and persistence, by activating the expression of the alg operon, AmrZ promotes this virulence phenotype. However, in response to changes in oxygen and nutrient levels or in the presence of toxic elements such as nitric oxide, AmrZ promotes cell dispersion from bacterial biofilms by activating the expression of several hydrolase and nuclease genes such as endA, pelA, and pslG [105]. AmrZ also plays a major role in motility, acting as a repressor of the type IV pili precursor pilA and fleQ, a c-di-GMP effector that activates the transcription of flagellum synthesis genes [22].
2.3. OCSs Regulating the Production and Secretion of Extracellular Enzymes, Toxins, and Toxic Secondary Metabolites
2.3.1. ExsA
ExsA belongs to the AraC family of regulators that mostly act as transcriptional activators upon signal sensing [106]. This regulator induces the expression of P. aeruginosa T3SS in response to low calcium levels, recruiting the RNAP complex to the promoter of the T3SS cluster [55]. This results in transcription from the 11 promoters controlling T3SS expression and secretion of the ExoT, ExoS, ExoU, and ExoY effectors (Table 1). ExsA was recently found to also activate the transcription of the impA gene that specifies a metalloprotease secreted by T2SS, which confers resistance to phagocytosis from macrophages [107]. In contrast to most AraC regulators that are activated by a small-molecule ligand, ExsA is part of the ExsACDE signaling cascade [55]. In non-induced conditions, ExsA is sequestered by ExsD, and the chaperone ExsC forms a complex with ExsE. Contact with host cells causes T3SS to secrete ExsE, switching ExsC to pair with ExsD instead. ExsA, now free from ExsD repression, activates gene expression [55]. Cytotoxicity assays showed that the cytotoxicity of a P. aeruginosa exsA mutant toward different cell lines was severely impaired [108,109]. Moreover, an exsA mutant is more susceptible to phagocytosis than the wild-type strain, likely due to the ExsA-dependent transcription of impA [107,110].
2.3.2. Sfa2
The Sfa2 protein is an EBP that activates the σ54-dependent transcription of P. aeruginosa H2-T6SS. Sfa2 binds to the enhancer-binding site on H2-T6SS genes, promoting the transcription of ~15 genes in this gene cluster, which includes the sfa2 gene itself [111]. A paralogue EBP regulator known as Sfa3 is encoded within the P. aeruginosa H3-T6SS gene cluster, suggesting a similar control of H3-T6SS expression by σ54 and this EBP [112].
2.3.3. GbdR
The AraC regulator GbdR activates the expression of hemolytic phospholipase C PlcH upon sensing glycine betaine and dimethylglycine [45]. PlcH produces phosphorylcholine, which is then dephosphorylated by PchP to release choline. Choline metabolism leads to the production of glycine betaine, which can either accumulate as an osmoprotector and a bacterial surfactant or be used as a nutrient source. GbdR acts as a switch for the transition between both fates upon accumulation of glycine betaine by promoting the expression of genes involved in choline conversion into serine and pyruvate [45]. Expression of the gbdR gene is activated by σRpoN and the EBPs CbrB and NtrC in the absence of carbon/nitrogen sources, and it is repressed by the betBA cluster repressor BetI in the absence of choline [113]. Although the role of GbdR in virulence has not been assayed to date, choline metabolism was shown to be important for the proper colonization and survival of P. aeruginosa in a mouse model of lung infection [19].
2.3.4. PvrA
The Pseudomonas virulence regulator PvrA belongs to the TetR family of transcriptional regulators that normally act as repressors of gene expression. Like GbdR, PvrA promotes the expression of the plcH phospholipase gene, although in response to fatty acyl-CoAs such as palmitoyl-CoA [52,114]. PvrA serves as a link between virulence and metabolism of phosphatidylcholine and long-chain fatty acids, one of the main carbon sources for P. aeruginosa during lung infection and a major component of the lung surfactant. In addition to directly controlling the expression of several metabolism genes and aprA alkaline protease, PvrA activates the production of pyocyanin and rhamnolipids by promoting transcription of the PQS biosynthesis operons of pqsABCDE and phnAB [52,114]. Moreover, it represses the expression of phaG encoding a protein involved in the conversion of 3-hydroxyacyl-acyl carrier protein (ACP), the substrate required for rhamnolipids production, into polyhydroxyalkanoate energy storage compounds [52]. In this way, PvrA acts as a switch diverting 3-hydroxyacyl-ACP use toward rhamnolipids production instead of energy storage. Accordingly, a mutation of pvrA was shown to significantly reduce the colonization and survival of P. aeruginosa in the lungs of mice [52,114].
2.3.5. SphR
SphR is an AraC family regulator that responds to sphingosine and activates the expression of genes that P. aeruginosa uses to feed on sphingolipids from the host cell membranes and the surfactant of mammalian lungs [54,115]. SphR activates the expression of the sphingolipid metabolism genes sphA and sphBCD and the ceramidase gene cerN [54,115]. In addition to enhancing the activity of PlcH phospholipase (Table 1), CerN is involved in the conversion of ceramide into sphingosine and fatty acids. P. aeruginosa uses fatty acids as a nutrient source, while sphingosine is used to attack host cells by disrupting the skin barrier [54]. SphR was found to be important for P. aeruginosa survival inside the lungs of mice, especially to overcome the antimicrobial effects of sphingosine [54,115].
2.3.6. PtxR
PtxR belongs to the LysR family of regulators, generally regarded as transcriptional activators of their target genes but with repressor activity on their own genes [116]. PtxR activates the transcription of the exotoxin A gene toxA and the lasB elastase and represses the transcription of rhlA rhamnolipids and phzA1 phenazine genes [43]. The activity of PtxR is modulated by the 2-ketogluconate sensor PtxS, which always acts as a repressor of gene expression regardless of PtxR’s role [117]. When PtxR acts as a repressor, PtxS binds to both DNA and PtxR, creating a DNA loop that promotes repressor activity. Repression is relieved by the presence of 2-ketogluconate, which releases both proteins from DNA. Conversely, when PtxR acts as an activator, PtxS binds to PtxR–DNA complexes, preventing gene expression. Upon detection of 2-ketogluconate, PtxS dissociates from PtxR, which activates gene expression [56,117].
2.3.7. SoxR
SoxR belongs to the MerR family of transcriptional regulators, which typically act as activators of gene expression [118]. SoxR regulators normally respond to oxidative stress by sensing superoxide; however, in P. aeruginosa, SoxR is activated by pyocyanin in a superoxide-independent manner and controls the release of phenazines and other chorismate-derived compounds [119,120]. To date, only two gene clusters have been identified as being part of the SoxR regulon: monooxygenase PA2274 and the multidrug efflux pump operon mexGHI-opmD [119,121]. The efflux pump MexGHI-OpmD was shown to increase the resistance to antibiotics such as norfloxacin and tetracycline, and it is involved in the export of a phenazine precursor required for pyocyanin production [122,123]. This efflux pump is also required for the production of virulence factors such as LasB elastase, rhamnolipids, pyocyanine, and pyoverdine, probably caused by the genetic response triggered by phenazines as signal molecules [122]. Accordingly, a P. aeruginosa soxR mutant was found to be attenuated for virulence in pulmonary and burn mouse models of infection [121,124].
2.4. OCSs Regulating the Acquisition and Homeostasis of Iron
2.4.1. Fur
The ferric uptake regulator Fur is the main regulator of iron homeostasis in P. aeruginosa. This regulator facilitates host infection by maintaining sufficient intracellular iron levels while avoiding iron toxicity. Upon binding iron, Fur directly represses the expression of iron acquisition genes and indirectly activates the expression of iron storage and detoxification genes, like the bacterioferritin gene bfrB, superoxide dismutase sodB, and catalase katA [61,62] (Table 1). Fur expands its regulation of iron homeostasis by repressing the expression of two sRNAs, i.e., PrrF1 and PrrF2, which block the production of over 50 proteins by binding their mRNA targets [61,85]. Fur represses the expression of pyoverdine and pyochelin biosynthesis and transport genes, which are thus only produced under iron-limited conditions [61]. Expression of the hemophore HasAp and the Phu, Has, and Hxu heme transport systems is also repressed by Fur under iron sufficient conditions. Moreover, Fur also represses the expression of other virulence factors including exotoxin A, the PIV/PrpL and IcmP proteases, extracellular LipA lipase, and hemolytic PlcH phospholipase C [50]. Fur is a global regulator that controls the expression of hundreds of genes involved not only in iron acquisition and virulence but also in some other aspects of P. aeruginosa biology such as respiration, metabolism, and stress responses [50].This wide spectrum of Fur targets explains why this protein is essential in P. aeruginosa for growth on solid media, especially considering the central role it plays in iron homeostasis [61,62].
2.4.2. PchR
PchR belongs to the AraC family of transcriptional regulators and activates the transcription of gene clusters pchDCBA and pchEF for pyochelin synthesis and fptABCX for pyochelin uptake [61]. The iron-loaded form of pyochelin acts as an effector molecule, activating the gene transcription of PchR targets, while PchR represses its own expression when siderophore is absent [61]. Pyochelin was shown to enhance growth and lethality of P. aeruginosa during infections, and a mutant defective in pyochelin and pyoverdine production was shown to be less virulent than a single-pyoverdine mutant [7]. In addition to pyochelin production, PchR controls the expression of the pqsABCDE gene cluster for PQS production and a number of virulence factors related to QS, such as the elastase lasB gene, the lectin lecA and lecB genes, and the gene clusters phzA1-G1 and hcnABC for phenazine and hydrogen cyanide production, respectively [28]. PchR is also involved in regulating the expression of other virulence-related genes, such as the algD gene for alginate production, the fimV gene for the synthesis of type IVa pili, and the bacterioferritin brfB gene [28].
2.4.3. AmpR
The β-lactam resistance regulator AmpR belongs to the LysR family of transcriptional regulators, and it is an important regulator of iron acquisition and the oxidative stress response [59]. AmpR acts as a global regulator, controlling the expression of more than 500 genes [59]. AmpR promotes iron acquisition by activating the transcription of regulatory anti-sense sRNA asPrrF1 (antagonizing the sRNA PrrF1), as well as the pyoverdine and pyochelin biosynthesis genes and the hemophore hasAp gene [59]. The oxidative stress response is mediated via AmpR by the activation of the katA catalase gene and the hydrogen peroxide resistance rgRgsA sRNA [59]. Consequently, an ampR mutation results in impaired growth under iron-limiting conditions and under H2O2-induced oxidative stress [59]. Moreover, AmpR is critical for P. aeruginosa to survive treatment with β-lactam antibiotics, and it also contributes to increasing P. aeruginosa’s “evolvability”, raising the chances of strengthening or developing new antibiotic resistances [125]. Under physiological conditions, AmpR is repressed by the cell wall precursor UDP-MurNAc pentapeptide. When β-lactam antibiotics cause cell wall damage, the muropeptides resulting from its restoration are internalized and bind AmpR to activate the transcription of the ampC β-lactamase gene [126]. Upon activation, AmpR promotes the expression of other key virulent determinants such as the HHQ transporter mexEF-oprN genes, the phz and hcn gene clusters for the production of pyocyanin and hydrogen cyanide, respectively, and several genes from T3SS and T6SS [59,127]. Accordingly, a P. aeruginosa ampR mutant was found to reduce mortality in the C. elegans infection model [127].
3. Control of P. aeruginosa Virulence by Two-Component Systems
Two-component systems (TCSs) are one of the main pathways for sensing and responding to environmental cues in prokaryotes, which are also present in eukaryotes [27]. Contrary to OCSs, the sensor and effector domains of TCSs are part of two different proteins. In a canonical TCS, the sensor protein is a membrane-embedded histidine kinase (HK) with the sensor domain facing outside the cytosol, which, upon signal recognition, phosphorylates a response regulator (RR) that mediates the response (Figure 1b) [128]. Next to the sensor and the transmembrane domains, HKs have a highly conserved cytosolic core kinase domain that, upon signal sensing, binds ATP and autophosphorylates in a conserved His residue [128]. Next, a phosphate transference reaction from the HK to its cognate RR occurs. Most RRs possess two main domains: a conserved N-terminal regulatory domain that receives the phosphate group from the His of the HK in an Asp residue and a variable C-terminal effector domain that generally binds to DNA [128]. The RR can act as a transcription activator or repressor, and it is common that the RR induces the transcription of the genes encoding a TCS [129]. The half-life of RR-P can range from seconds to hours, and its activity is turned off by phosphatases that hydrolyze phosphate [128]. Many RRs have autophosphatase activity that decreases the lifetime of the phosphorylation, and several HKs also possess phosphatase activity and are able to dephosphorylate their cognate RRs [128]. Important variations from this archetypal model of TCSs have evolved over time. The most outstanding modification is the existence of kinases with numerous phosphodonor sites [128]. The succession of several phosphorelay steps facilitates an accurate control of the pathway activation and reduces the occurrence of non-specific cross-talk because of the presence of several regulatory checkpoints [128]. The genome of P. aeruginosa PAO1 encodes 41 HKs and 68 RRs, having an additional 18 hybrid proteins exhibiting domains from both types of proteins [92]. Although TCSs in this pathogen have been studied for a long time, the specific signals activating these pathways have been rarely identified, and several remain unknown.
3.1. TCSs Regulating Motility, Attachment, and Biofilm Formation
3.1.1. PilS-PilR
The TCS PilSR is the main regulator of the pilABCD cluster controlling type IVa pili motility [25]. The HK PilS interacts with the pilin subunit PilA, detecting changes in its abundance and controlling its production. When PilA is not detected, this results in a higher activity of the RR PilR in order to replenish the PilA pool in the cell. RNAseq analyses using a pilA mutant, in which PilR was constitutively active and a pilR mutant, allowed the identification of 10 genes that were inversely regulated in both strains, including the hcpA and hcpB effectors for T6SS [21]. In addition to controlling type IVa pili motility, the PilSR system plays a role in swimming motility by regulating the expression of the flagellum TCS FleSR [21].
3.1.2. FleS-FleR
FleSR activates the transcription of the basal body, hook, and filament proteins of flagella, and it is thereby important for swimming motility [48]. Additionally, this TCS enhances P. aeruginosa cytotoxicity because it promotes the expression of toxA exotoxin, lasB elastase, and T3SS, likely via the ExsA regulator [48]. However, it is not clear how FleSR controls AmrZ, as no direct binding of the RR to the promoter has been observed [130]. Expression of the fleSR gene cluster depends on the diguanylate effector FleQ to control its transcription in a c-di-GMP dependent manner as well as on the TCS PilSR [21,131].
3.1.3. RocS-RocR-RocA1
The HK RocS controls the expression of the cupB and cupC systems (Table 1) by interacting with two different RRs: the activator RocA1 and the repressor RocR [29,132]. RocA1 activation follows the classical TCS phosphorylation-dependent mechanism, with phosphorylated RocA1 acting as a transcriptional activator of the cupB and cupC gene clusters [133]. RocR repression of the cupB and cupC systems depends on c-di-GMP levels, and this regulator contains a C-terminal EAL domain to sense and catalyze this signal molecule [133]. It has been suggested that the activation of c-di-GMP phosphoesterase activity of RocR takes place upon phosphorylation by RocS and that gene repression may happen by competition with RocA1 for the HK [133].
3.1.4. RcsC-RcsB and PvrS-PvrR
The TCSs RcsCB and PvrSR control the expression of the P. aeruginosa PA14 strain-specific cupD gene cluster, with their RRs RcsB and PvrR acting as an activator and a repressor, respectively, of the transcription of this system [30]. In the most recent model, the HK RcsC transfers a phosphate group from the HK PvrS to the RR RcsB [30]. Phosphorylated RcsB activates the transcription of cupD, as well as other target genes such as the DNA-binding PA3714 and PA0034 proteins [30]. RcsC does not only mediate in the phosphotransference between PvrS and RcsB, but it also has an inhibitory role as a phosphatase to inactivate RcsB [30]. The remaining RR, PvrR, is a c-di-GMP-dependent repressor of the cupD cluster, with a C-terminal EAL domain similar to the RocR repressor, suggesting a similar mechanism for PvrR repression. [30]. All four proteins of this intertwined signaling network are required for P. aeruginosa virulence because single mutations in any of these genes were found to considerably reduce mortality in a burn mouse model of infection [134].
3.1.5. PprA-PprB
The cupE cluster is regulated by the Pseudomonas permeability regulator PprAB [11]. The RR PprB induces the expression of the cupE operon and several genes from the type IVb pili apparatus, such as the tad locus, the flp pilin, and the adhesin bapABCD gene cluster [12]. PprAB also indirectly affects the expression of the PQS system, thus having a broader impact on virulence [12]. Because of this, overexpression of the PprB protein was found to cause a hyper-biofilm phenotype, higher susceptibility to antibiotics such as tobramycin due to a higher membrane permeability, and attenuated virulence in a fruit fly infection model [12].
3.1.6. FimS-AlgR
FimS-AlgR controls alginate production via the activation of the algC gene and algD operon for alginate biosynthesis and transport [135] (Figure 3). This TCS also induces expression of the fimU operon involved in type IVa pili motility and the QS-related genes rhlI, rhlA, and hcnA [135]. The HK FimS interacts with the surface contact sensor PilJ, which senses tension changes in the pili via the PilA protein-activating FimS, which in turn phosphorylates the RR AlgR [136]. AlgR can function as a repressor or an activator of gene transcription depending on its phosphorylation state, which seems to depend on the stage of infection. In early stages of biofilm formation, AlgR is phosphorylated and functions as an activator favoring motility and cell attachment. Unphosphorylated AlgR acts as a repressor and is more important in late-stage biofilms. AlgR also promotes mucR expression, a diguanylate cyclase that alters c-di-GMP levels, thus promoting biofilm formation and the establishment of chronic infections [135]. Mutations in either fimS or algR result in a nearly complete loss of twitching motility [135]. The fimS or algR mutants were found to display similar killing rates as the wild-type, but a phosphomymetic mutant of AlgR showed delayed killing in a C. elegans model [137].
3.1.7. KinB-AlgB
The TCS KinB-AlgB also controls alginate production by activating the expression of the alginate biosynthetic cluster (Figure 3) [40]. The unphosphorylated form of the RR AlgB binds to three sites on the algD promoter, activating its transcription. Mutation of the HK kinB gene results in alginate overproduction due to the constitutive activation of AlgB that, in this mutant, cannot be inactivated by phosphorylation [40]. This mutation also causes defects in the acute phase of infection, producing fewer QS-regulated virulence factors, such as pyocyanin and elastase, and showing impaired swimming motility and biofilm formation [138]. KinB has both kinase and phosphatase activity, playing a dual role in the phosphorylation of AlgB. In the phosphorylated form, AlgB represses acute virulence and alginate production. Dephosphorylation of this protein by KinB promotes these phenotypes during the acute infection stage [139]. While a kinB mutation was found to decrease P. aeruginosa virulence in zebrafish embryo and murine models of infection, algB single- or kinB/algB double-mutants produce similar mortality rate compared to the wild-type strain [138,139,140].
3.1.8. GacS-GacA
The GacSA TCS governs switching from acute to chronic P. aeruginosa infection by activating the transcription of the rsmY and rsmZ genes. These two sRNA genes are the only targets identified for this TCS, and they modulate the activity of the global regulator RsmA (Figure 4) [24]. RsmA is a RNA-binding protein that blocks the translation of genes for biofilm formation such as the pel and psl EPS genes, while indirectly activating transcription of motility and virulence factors such as the type IV pili and T3SS genes [24]. RsmA is thus one of the main proteins controlling the transition from a planktonic to a sessile lifestyle, which correlate with the acute and chronic stages of infection, respectively. The sRNAs rsmY and rsmZ bind to RsmA, preventing the binding to its targets, and thus promoting biofilm formation and the development of a chronic infection [24].
The activity of the HK GacS is modulated by two additional HKs, RetS and LadS (Figure 4) [141,142]. RetS is a dimeric HK that binds to a GacS homodimer to form a heterocomplex that represses GacS activity, thereby promoting motility and virulence. The HK PA1611 seems to intercede in the formation of this heterocomplex by forming a heterodimer with RetS (Figure 4). LadS is a HK that can transfer a phosphate to GacS, activating its signaling cascade and promoting biofilm formation while attenuating virulence [142,143]. Whereas no signal has been yet identified for GacS, calcium seems to be the inducer of the LadS-dependent activation of the GacSA cascade, and mucins and associated glycans (normally covering epithelial cells) are the signals sensed by RetS to inhibit GacS [142,144].
The P. aeruginosa PA14 strain harbors a truncation in the LadS HK, which seems to partially account for the increased cytotoxicity of this strain compared to other strains such as PAO1 [141]. Virulence assays using the Galleria mellonella larvae model of infection showed that a gacS deletion did not have a significant effect on virulence. However, a deletion of the HAMP domain, transducing the signal from the periplasmic sensor to the cytoplasmic kinase domain, resulted in an important drop in mortality [145]. Likewise, deleting the HK gene retS increased larvae survival, implying that either the HAMP domain of GacS is important for RetS inhibitory activity or a mutant in the HAMP domain results in hyperactive GacS [145].
3.1.9. BfiS-BfiR, BfmS-BfmR, and MifS-MifR
The TCSs BfiSR, BfmSR, and MifSR act sequentially in the irreversible attachment and maturation stages 1 and 2 of the biofilm formation process [27]. BfiSR regulates irreversible attachment by activating the transcription of the RNAse G cafA gene, which reduces rsmZ levels, thereby enhancing RsmA activity (Figure 4) [27]. The BfiS HK also interacts with the hybrid HK SagS, which seems to act by modulating the phosphorylation state of BifS [146]. A bfiS mutant was found to reduce mortality in a plant infection model, while mutation of its regulatory kinase SagS produced a hypervirulent phenotype [146]. Furthermore, BfmSR regulates biofilm maturation by activating the expression of the prevent-host-death gene phdA, thus suppressing the premature release of eDNA [14]. The BfmR RR controls the expression of other transcriptional regulators. BfmR represses the expression of the QS rhlR gene and the Zn2+ sensing czcRS TCS, controlling phenazine production, and induces the expression of the T3SS regulator exsA [147,148]. The virulence of a bfmS mutant was found to be severely impaired, while the virulence of a double-bfmSR mutant was similar to the wild-type strain, probably by relieving BfmR repression on virulence regulators [147]. Lastly, the MifSR TCS affects microcolony and cluster formation during the last stages of the biofilm maturation process. Upon sensing α-ketoglutarate, the HK MifS phosphorylates MifR, which acts as an EBP, assisting the σ54 factor RpoN to bind to the promoter region of α-ketoglutarate transport genes [27,149]. α-ketoglutarate is a preferred carbon source for P. aeruginosa, and it is required for virulence in pathogenic bacteria [149]. MifR has also been shown to alter the production of at least 18 proteins when mutated or overexpressed, many of which are involved in growth under anaerobic conditions, suggesting a link between biofilm formation and oxygen restriction [42]. In a pneumonia mouse model, a mifR mutant showed significant defects in virulence and mortality, with fewer lung injuries, a lower inflammatory response, and reduced cell death [150].
3.1.10. GtrS-GltR
The glucose uptake and metabolism TCS GtrS-GltR controls rsmY and rsmZ levels and, thereby, has an effect on the RmsA-mediated phase switch and virulence [26]. By inducing the expression of the oprB gene for glucose uptake, pyruvate metabolism is activated, which leads to a repression of rsmY and rsmZ expression [26]. In a mouse model of acute pneumonia and a fruit fly infection model, a P. aeruginosa gtrS mutant was impaired for colonization and dissemination and had reduced mortality, mirroring the virulence traits of a rsmA mutant [26,151]. Activation of the HK GtrS depends on the binding to 2-ketogluconate or phosphogluconate, although it seems to also respond to glucose via the periplasmic protein GltB [57,151]. GltR represses the expression of several genes that are essential for metabolism, such as the glk and edd/gap-1 genes, and for virulence, such as the exotoxin toxA gene [57,151].
3.2. TCSs Regulating the Production and Secretion of Extracellular Enzymes, Toxins, and Toxic Secondary Metabolites
PhoR-PhoB
Under phosphate limitation, the TCS PhoRB activates the expression of genes involved in phosphate assimilation and metabolism, including the hxc-T2SS operon and the pstSCAB-phoU transport system [152,153]. Hxc-T2SS acts as an alternative system to scavenge inorganic phosphate under phosphate-limiting conditions by secreting alkaline phosphatases to the extracellular space [154]. Phosphate depletion has shown to dramatically increase virulence in P. aeruginosa both in surgical injuries and animal infection models and, conversely, hosts treated with phosphate supplements have shown to be less susceptible to P. aeruginosa infections [155,156]. When phosphate conditions are favorable, the PhoU subunit of the Pst transport complex interacts with the HK PhoR to prevent its autophosphorylation [152,153]. In response to low phosphate, PhoR phosphorylates the PhoB RR, which, in addition to genes involved in phosphate transport and metabolism, activates the transcription of H2- and H3-T6SS and the three QS systems [157]. Consequently, PhoB promotes pyocyanin production and internalization by epithelial cells in low-phosphate conditions [157]. It was found that clinical isolates from P. aeruginosa with a PhoB-dependent formation of PstS rich structures resulted in a hypervirulent phenotype, causing the death of 60% of surgically injured mice. Phosphate depletion during P. aeruginosa infection of C. elegans results in the so-called “red death”, a lethal phenotype resulting from the combined activation of PhoB, PqsR, and the pyoverdin acquisition systems [156].
3.3. TCSs Regulating the Acquisition and Homeostasis of Iron
BqsS-BqsR
The TCS BqsSR responds to extracellular Fe2+ and activates its own expression by triggering the expression of the bqsPQRST cluster and that of spermidine, ferric reductases, carbonic anhydrases, and sulphate transporter genes [158,159]. This TCS promotes the synthesis of rhamnolipids and pyocyanin by increasing the production of C4HSL and PQS signaling molecules [158,159]. The reduced Fe2+ form activating BqsSR is scarce in the oxygenic environments that conform the usual habitats of P. aeruginosa, where iron is usually found as Fe3+. However, it was observed that Fe2+ locally accumulates in microenvironments in the lungs of CF patients and that it further accumulates by the increase in cell density and phenazine production, providing a link between QS, iron acquisition, and virulence [158,160].
4. Control of P. aeruginosa Virulence by Sigma (σ) Factors
Contrary to OCSs and TCSs, which modulate transcription by binding to operator sequences, σ factors affect gene expression by directly recruiting the RNAP core enzyme to their promoter regions and then starting transcription. As is customary for most bacterial species, P. aeruginosa produces a single σ54 factor known as σRpoN. This σ54 factor recognizes the promoter region of hundreds of genes with the assistance of up to 22 different EBPs (depending on the strain) (e.g., the OCS Sfa2 or the RRs AlgB, PilR, or FleR) [20,111,140]. Conversely, bacteria typically produce several σ factors of the σ70 family. Alternative σ factors promote the transcription of functions required only under specific conditions, and their activity is controlled by specific stimuli. This control can be exerted at different levels (i.e., by modulating expression, covalent modifications, proteolysis), although it is particularly common the post-translational control of alternative σ factors by an anti-σ factor that prevents the interaction of the σ factor with the DNA and RNAP core enzyme in absence of the stimulus (Figure 1c). P. aeruginosa produces one primary σ factor encoded by the rpoD gene and between 22 and25 alternative σ factors, depending on the strain. Of those, Group 4, containing the so-called extracytoplasmic function σ (σECF) factors, is the most numerous and diverse group, with P. aeruginosa encoding between 19 and 21 σ factors in this group [161].
4.1. σ Factors Regulating Motility, Attachment, and Biofilm Formation
4.1.1. σFliA (σ28)
The σFliA factor controls the expression of the flagella biosynthesis genes of P. aeruginosa and is thus essential for bacteria motility. Its activity is modulated by its cognate anti-σ factor FlgM via partner switching with the protein HsbA, which acts as an anti-σ factor antagonist when unphosphorylated [162]. The phosphorelay system leading to HsbA phosphorylation is intertwined with the RsmA signaling network, acting as a switch between a motile planktonic lifestyle when unphosphorylated and biofilm formation when phosphorylated [20,162]. In addition to being essential for flagella-driven swimming motility, σFliA is important for swarming motility [163]. A transcriptomic study on a P. aeruginosa fliA mutant of the PAO1 strain showed that several effector genes of Xcp-T2SS (i.e., lipC lipase and phlC phospholipase C), H2-T6SS (i.e., vgrG, hcp, clpV2, and lip2), and T3SS (i.e., exsC, exsE, and pscBCD) were strongly downregulated, showing that this σ factor is required for the expression of these virulence factors [53]. However, σFliA represses the expression of other virulence determinants such as pyocyanin [53]. Interestingly, a fliA mutant of the hypervirulent P. aeruginosa B136–33 strain showed less capacity for adhesion to Caco-2 monolayer cells and a lower invasion activity in MDCK cells, and its capacity to colonize mice was considerably reduced [53]. σFliA is essential for successful adhesion and infection of H9C2 cardiomyocyte cells of the PAO1 strain, which further supports the crucial role of flagella in pathogenesis through motility [164].
4.1.2. σSigX
The σSigX factor regulates the attachment and adherence of P. aeruginosa to host tissues by controlling the swarming and twitching motility of this bacterium [23,165]. It was proposed that σSigX activity is controlled by a mechanosensing complex involving the anti-σ factor CfrX, the outer membrane porin OprF, and the ion channel CmpX [166]. The lack of this σ factor decreases the expression of pilA and flp genes, encoding the pilin subunits of the type IVa and IVb pili, respectively, and the tad operon, encoding the machinery that assembles type IVb pili [23]. Furthermore, the expression of the RR gene pprB, which promotes the transcription of the flp type IVb pilin, the bap adhesion system genes, and the cupE CUP pili cluster, was found to be downregulated in a sigX mutant [23]. Consequently, the lack of σSigX reduces the biofilm formation capacity of P. aeruginosa [23]. A sigX mutation impairs the production of other virulence factors, like exotoxin A, pyocyanin, the T1SS-related genes aprA, aprD, and aprX, Xcp-T2SS, and H3-T6SS [23]. Accordingly, a P. aeruginosa sigX mutant was found to be less cytotoxic towards A549 epithelial pulmonary cells and Caco2/TC7 intestine cells than the wild-type strain [23,165].
Furthermore, σSigX regulates the expression of several fatty acid biosynthesis genes, thus playing a crucial role in membrane fluidity and homeostasis [166]. Importantly, many genes of the σSigX regulon are associated with cell membrane integrity, thus suggesting that σSigX could regulate the response of P. aeruginosa to membrane-disrupting antimicrobials. Interestingly, a sigX mutant strain showed considerably higher resistance to the antimicrobials tetracycline, nalidixic acid, and erythromycin [23].
Finally, σSigX also plays a key role in regulating iron uptake by promoting the expression of the prrF1 sRNA gene, which represses iron sequestration under iron-starvation conditions [51,167]. Furthermore, the absence of σSigX was found to cause a reduced expression of the pyochelin biosynthesis genes and of several TBDTs involved in iron (ChtA, FpvA and FpvB) and heme uptake (PhuR) [23,168].
4.1.3. σAlgT
The σAlgT factor is the main regulator of the alg operon encoding the biosynthetic pathway of the EPS alginate, a main component of the extracellular matrix of P. aeruginosa biofilms [169] (Figure 3). This σ factor, which is a homologue to the stress-responsive σE factor of E. coli, is kept inactive under non-stress conditions by the anti-σ factor MucA encoded within the algT-mucABCD operon [161]. The presence of unfolded proteins leads to the activation of σAlgT via regulated intramembrane proteolysis (RIP) by the DegG and RseP proteases (Figure 3) [161]. The main cause of the mucoid phenotype of P. aeruginosa is a mutation of the mucA gene, which results in the constitutive activation of σAlgT and continuous production of alginate [170]. In addition to activating alginate production, σAlgT controls biofilm formation by modulating the expression of the of the psl EPS gene. Basal σAlgT concentrations activate the expression of psl, while high σAlgT concentrations induce the transcription of the biofilm inhibitor rsmA gene, which prevents the production of the Psl EPS [41,169]. σAlgT also activates the expression of lecB, which codifies for a lectin involved in biofilm formation and post-transcriptional type IVa pili biogenesis [33]. Furthermore, σAlgT affects the attachment to solid surfaces via induction of the paerucumarin pvcA gene, which enhances expression of the cup adhesion genes [171].
σAlgT also regulates the expression of several other virulence determinants such as the LasB and the AprA proteases and the hcn hydrogen cyanide biosynthesis genes [46,172]. Several genes encoding pro-inflammatory lipoproteins (i.e., lptA, lptE, and osmE) are induced in mucoid P. aeruginosa strains because their expression is promoted by σAlgT, which causes excessive inflammation in CF patients [58,172]. Moreover, the genes slyB, encoding a putative porin involved in membrane integrity, osmC, encoding a peroxiredoxin inducible by osmotic stress, and pfpI, encoding a protease that protects against oxidative stress, are differentially expressed in mucoid P. aeruginosa strains in a σAlgT-dependent manner [46,166,172]. Furthermore, σAlgT regulates the expression of the heat-shock response σRpoH factor. The regulation of all these genes related to adaptation against several stresses by σAlgT provides an advantage for this pathogen that can overcome the host immune response during infection.
The σAlgT regulon includes a wide assortment of regulatory genes, reflecting the complex σAlgT signaling network. This network includes several OCSs and TCSs, such as the alginate regulators AmrZ, KinB/AlgB, and FimS/AlgR as well as σECF factors such as σSbrI involved in motility and biofilm formation [51]. σAlgT indirectly controls motility by inducing amrZ expression, which, subsequently, represses fleQ, encoding for the major regulator of flagella biosynthesis [22]. In a mucA mutant strain, σAlgT activation was found to cause downregulation of the virulence factor regulator gene vfr via the RR AlgR [173].
4.1.4. σRpoH (σ32)
The σRpoH factor is responsible of the P. aeruginosa response to heat-shock and accumulation of misfolded proteins [20]. σRpoH is essential for viability in P. aeruginosa and induces the expression of several virulence factors including the mucA and mucB genes, which modulate the activity of the σAlgT factor, and the global regulator RsmA controlling biofilm formation [38]. Furthermore, this σ factor positively regulates the expression of several chaperones, proteases, and accessory proteins, such as ClpX, ClpB, FtsH, and GrpE, that are required for refolding or degradation of non-functional proteins [20,38]. Overproduction of σRpoH significantly increases the sensitivity of P. aeruginosa to gentamicin and other aminoglycoside antibiotics such as tobramycin in biofilms [38]. Interestingly, a transcriptome study on P. aeruginosa biofilms in a mouse wound model showed that much of the heat-shock response genes regulated by σRpoH are induced in vivo during infection [174].
4.1.5. σRpoS (σ38)
The σRpoS factor is responsible for regulating the global stress response of P. aeruginosa. The activity of this alternative σ factor is induced during the stationary phase and modulates the expression of more than 700 genes of many functional categories [175]. σRpoS directly regulates the expression of the RR PprB which, in turn, promotes the expression of several cell adhesion and motility-related genes, as described before [31]. Moreover, σRpoS positively regulates biofilm formation by promoting the expression and production of the EPS Psl and alginate [36,37]. Surprisingly, the rpoS mutation was found to cause a hyperbiofilm phenotype and heightened virulence of P. aeruginosa in a macrophage cytotoxicity model [176]. This hyperbiofilm phenotype could be caused by the increase in c-di-GMP observed in P. aeruginosa rpoS mutants [31]. The expression of rpoS is highly induced in P. aeruginosa isolates from CF patients [177]. In accordance, a P. aeruginosa rpoS mutant was found to increase virulence and cause more lung damage than the wild-type strain in a rat chronic-infection model [36].
4.1.6. σSbrI
The σECF factor SbrI is under the control of the anti-σ factor SbrR and controls swarming motility and biofilm formation in P. aeruginosa [35]. σSbrI promotes the transcription of the muiA (from mucoidy inhibitor A) gene, which encodes the MuiA protein that inhibits alginate production by interfering with the PalgD promoter activity [35]. However, the phenotypic characterization of a sbrR mutant, which leads to constitutive activation of σSbrI, showed that this σ factor inhibits swarming motility and enhances biofilm formation, suggesting that the role of MuiA as an inhibitor of alginate production and biofilm formation should be re-evaluated [35]. σSbrI and MuiA are overexpressed under osmotic shock conditions, after treatment with the cell-wall inhibitory antibiotic D-cycloserine, and following overexpression of the periplasmic protease CtpA [161,178]. Altogether, these findings strongly suggest that σSbrI plays a role in the cell-envelope stress response and directs the transition from motile cells to biofilm growth [35].
4.2. σ Factors Regulating the Production and Secretion of Extracellular Enzymes, Toxins, and Toxic Secondary Metabolites
σVreI
The σVreI factor controls the expression of Hxc-T2SS and the PdtA/PdtB two-partner secretion (TPS) system, among other genes [179]. These secretion systems mediate the secretion of low-molecular-weight alkaline phosphatases LapA, LapB, and LapC [180] and the high-molecular-weight surface-exposed protein PdtA [152], respectively. Importantly, overproduction of this σ factor was found to enhance P. aeruginosa virulence in a zebrafish embryo model of infection [179]. σVreI is expressed under inorganic phosphate (Pi)-starvation conditions together with the VreR anti-σ factor in a PhoB-dependent manner [152]. PhoB also promotes the transcription of σVreI regulon genes by binding to DNA and enhancing the binding of σVreI-loaded RNAP [152]. Antibodies against proteins of the σVreI regulon are found in the serum of CF patients, suggesting that this σ factor is active during infection [179]. This is supported by the fact that σVreI is active during P. aeruginosa infection of zebrafish embryos and lung epithelial cells [155]. Importantly, the lack of vreI was found to significantly reduce virulence of P. aeruginosa in zebrafish embryos and its cytotoxicity towards lung epithelial cells [155].
4.3. σ Factors Regulating the Acquisition and Homeostasis of Iron
4.3.1. σPvdS and σFpvI
The σPvdS factor promotes the transcription of the pyoverdine biosynthetic locus, the main siderophore produced by P. aeruginosa. Furthermore, it was found that there is a correlation between genes required for biofilm formation and for pyoverdine production and that biofilm formation may be necessary for full pyoverdine production [181]. Transcriptomic analyses in presence of phenazines and oxidative stress showed that pyoverdine signaling may also be connected to these other aspects of P. aeruginosa pathogenesis and stress responses [119,182]. In addition to the pyoverdine biosynthetic locus, the P. aeruginosa σPvdS regulon comprises about 80 genes [51]. σPvdS activates the transcription of several extracellular virulence factors such as exotoxin A, PIV/PrpL protease, the T3SS effectors ExoT and ExoS, and the T3SS major regulator ExsA [51,183]. Inhibiting the expression of σPvdS was found to suppress P. aeruginosa pathogenicity in a mouse model of lung infection, evidencing the link between σPvdS and virulence [184].
Activity of σPvdS is controlled by the FpvR anti-σ factor and the FpvA outer membrane receptor in response to the presence of pyoverdine [185]. This signaling cascade also controls the activation of the σFpvI factor, which promotes the expression of the fpvA gene and several pyoverdine biosynthesis genes, the hasR heme receptor gene, and the prrF1 sRNA gene [169].
4.3.2. σHasI and σHxuI
P. aeruginosa possesses two IS σECF factors that respond to the host iron carrier heme, i.e., σHasI and σHxuI [81]. While σHxuI is activated by free heme, σHasI is activated by the heme–HasAp hemophore complex [81]. Several genes in the Has and Hxu systems are highly expressed in vivo in murine models of acute and chronic infection [186,187]. In line with this, the lack of the heme receptor HasR was found to reduce P. aeruginosa growth in a mouse model of acute infection [186]. The σHxuI regulon includes several virulence genes such as lasA elastase, lecB lectin, and the phenazine biosynthetic cluster [32]. Importantly, the Hxu system has shown to be particularly active during bloodstream infections in P. aeruginosa clinical isolates, and its ability to cause sepsis is greatly reduced in hxuI mutants, while it is enhanced in σHxuI overproducing strains [61]. The lack of σHxuI was also found to reduce P. aeruginosa acute infection in mice, and overexpression of σHxuI was found to promote long-term infection in a murine model [32].
5. Concluding Remarks
The emergence and spread of antibiotic resistance among pathogenic bacteria is one of the greatest health threats worldwide. The ability of P. aeruginosa to resist multiple antibiotics has ranked it among the critical priority pathogens for the development of new therapeutic approaches [61]. Pathogenesis in P. aeruginosa originates from the multi-layered combination of more than 30 virulence factors coordinated to infect and colonize virtually any human tissue (summarized in Table 1). Success in this infectious process depends on the timely production of these virulence determinants in response to the host environment and the proper use of this battery of resources to evade the host’s defenses. This is achieved by a tightly knit network of transcriptional regulators orchestrating gene expression across the different stages of the infectious process. Disrupting the signaling cascades that coordinate the expression of virulence genes has arisen as a promising alternative strategy to treat P. aeruginosa infections, as tampering with most of these transcriptional regulators results in significant drops in virulence. Many of the new therapies being developed against this pathogen are based on targeting such virulence factors and their regulators. Along these lines, several compounds have been discovered to act as QS inhibitors by blocking AHL sensing and/or production or as structural analogues of PQS that inhibit quinolone signaling in P. aeruginosa [44]. Disrupting iron homeostasis is also flourishing as a new therapy to treat P. aeruginosa infections with the use of iron mimics and chelators, and iron acquisition mechanisms are being used to deliver antibiotic compounds with the so-called ‘Trojan-horse’ strategy [61]. Drugs targeting other virulence hallmarks of P. aeruginosa such as biofilm formation or its secretion systems and effectors are also under development [188]. Thus, understanding the signaling pathways controlling virulence is critical to the discovery of novel drugs against this pathogen based on the inhibition or activation of its transcriptional regulators.
Author Contributions
Conceptualization, M.A.L. and F.J.M.-T.; writing—original draft preparation, A.S.-J., M.A.L. and F.J.M.-T.; writing—review and editing, A.S.-J., M.A.L. and F.J.M.-T. All authors have read and agreed to the published version of the manuscript.
Funding
This work was funded by MCIN/AEI/10.13039/501100011033 Spanish agency with project PID2020-115682GB-I00.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Not applicable.
Conflicts of Interest
The authors declare no conflict of interest.
References
- Morin, C.D.; Déziel, E.; Gauthier, J.; Levesque, R.C.; Lau, G.W. An Organ System-Based Synopsis of Pseudomonas aeruginosa Virulence. Virulence 2021, 12, 1469–1507. [Google Scholar] [CrossRef]
- Muggeo, A.; Coraux, C.; Guillard, T. Current Concepts on Pseudomonas aeruginosa Interaction with Human Airway Epithelium. PLoS Pathog. 2023, 19, e1011221. [Google Scholar] [CrossRef]
- Buhl, M.; Peter, S.; Willmann, M. Prevalence and Risk Factors Associated with Colonization and Infection of Extensively Drug-Resistant Pseudomonas aeruginosa: A Systematic Review. Expert Rev. Anti-Infect. Ther. 2015, 13, 1159–1170. [Google Scholar] [CrossRef] [PubMed]
- Hauser, A.R.; Jain, M.; Bar-Meir, M.; McColley, S.A. Clinical Significance of Microbial Infection and Adaptation in Cystic Fibrosis. Clin. Microbiol. Rev. 2011, 24, 29–70. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Livermore, D.M. Multiple Mechanisms of Antimicrobial Resistance in Pseudomonas aeruginosa: Our Worst Nightmare? Clin. Infect. Dis. 2002, 34, 634–640. [Google Scholar] [CrossRef] [Green Version]
- Karakonstantis, S.; Kritsotakis, E.I.; Gikas, A. Pandrug-Resistant Gram-Negative Bacteria: A Systematic Review of Current Epidemiology, Prognosis and Treatment Options. J. Antimicrob. Chemother. 2020, 75, 271–282. [Google Scholar] [CrossRef]
- Chadha, J.; Harjai, K.; Chhibber, S. Revisiting the Virulence Hallmarks of Pseudomonas aeruginosa: A Chronicle through the Perspective of Quorum Sensing. Environ. Microbiol. 2021, 24, 2630–2656. [Google Scholar] [CrossRef]
- Khan, F.; Pham, D.T.N.; Oloketuyi, S.F.; Kim, Y.M. Regulation and Controlling the Motility Properties of Pseudomonas aeruginosa. Appl. Microbiol. Biotechnol. 2020, 104, 33–49. [Google Scholar] [CrossRef]
- Burrows, L.L. Pseudomonas aeruginosa Twitching Motility: Type IV Pili in Action. Annu. Rev. Microbiol. 2012, 66, 493–520. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Webster, S.S.; Wong, G.C.L.; O’Toole, G.A. The Power of Touch: Type 4 Pili, the von Willebrand A Domain, and Surface Sensing by Pseudomonas aeruginosa. J. Bacteriol. 2022, 204, e00084-22. [Google Scholar] [CrossRef]
- Giraud, C.; Bernard, C.S.; Calderon, V.; Yang, L.; Filloux, A.; Molin, S.; Fichant, G.; Bordi, C.; De Bentzmann, S. The PprA-PprB Two-Component System Activates CupE, the First Non-Archetypal Pseudomonas aeruginosa Chaperone-Usher Pathway System Assembling Fimbriae. Environ. Microbiol. 2011, 13, 666–683. [Google Scholar] [CrossRef]
- De Bentzmann, S.; Giraud, C.; Bernard, C.S.; Calderon, V.; Ewald, F.; Plésiat, P.; Nguyen, C.; Grunwald, D.; Attree, I.; Jeannot, K.; et al. Unique Biofilm Signature, Drug Susceptibility and Decreased Virulence in Drosophila through the Pseudomonas aeruginosa Two-Component System PprAB. PLoS Pathog. 2012, 8, 1003052. [Google Scholar] [CrossRef]
- Chemani, C.; Imberty, A.; De Bentzmann, S.; Pierre, M.; Wimmerová, M.; Guery, B.P.; Faure, K. Role of LecA and LecB Lectins in Pseudomonas aeruginosa-Induced Lung Injury and Effect of Carbohydrate Ligands. Infect. Immun. 2009, 77, 2065–2075. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Petrova, O.E.; Schurr, J.R.; Schurr, M.J.; Sauer, K. The Novel Pseudomonas aeruginosa Two-Component Regulator BfmR Controls Bacteriophage-Mediated Lysis and DNA Release during Biofilm Development through PhdA. Mol. Microbiol. 2011, 81, 767–783. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ramsey, D.M.; Wozniak, D.J. Understanding the Control of Pseudomonas aeruginosa Alginate Synthesis and the Prospects for Management of Chronic Infections in Cystic Fibrosis. Mol. Microbiol. 2005, 56, 309–322. [Google Scholar] [CrossRef] [PubMed]
- Davey, M.E.; Caiazza, N.C.; O’Toole, G.A. Rhamnolipid Surfactant Production Affects Biofilm Architecture in Pseudomonas aeruginosa PAO1. J. Bacteriol. 2003, 185, 1027. [Google Scholar] [CrossRef] [Green Version]
- Van Gennip, M.; Christensen, L.D.; Alhede, M.; Phipps, R.; Jensen, P.Ø.; Christophersen, L.; Pamp, S.J.; Moser, C.; Mikkelsen, P.J.; Koh, A.Y.; et al. Inactivation of the RhlA Gene in Pseudomonas aeruginosa Prevents Rhamnolipid Production, Disabling the Protection against Polymorphonuclear Leukocytes. APMIS 2009, 117, 537–546. [Google Scholar] [CrossRef] [Green Version]
- Zulianello, L.; Canard, C.; Köhler, T.; Caille, D.; Lacroix, J.S.; Meda, P. Rhamnolipids are Virulence Factors That Promote Early Infiltration of Primary Human Airway Epithelia by Pseudomonas aeruginosa. Infect. Immun. 2006, 74, 3134–3147. [Google Scholar] [CrossRef] [Green Version]
- Wargo, M.J. Choline Catabolism to Glycine Betaine Contributes to Pseudomonas aeruginosa Survival during Murine Lung Infection. PLoS ONE 2013, 8, e56850. [Google Scholar] [CrossRef] [Green Version]
- Potvin, E.; Sanschagrin, F.; Levesque, R.C. Sigma Factors in Pseudomonas aeruginosa. FEMS Microbiol. Rev. 2008, 32, 38–55. [Google Scholar] [CrossRef] [Green Version]
- Kilmury, S.L.N.; Burrows, L.L. The Pseudomonas aeruginosa PilSR Two-Component System Regulates Both Twitching and Swimming Motilities. MBio 2018, 9, 1–13. [Google Scholar] [CrossRef] [Green Version]
- Tart, A.H.; Blanks, M.J.; Wozniak, D.J. The AlgT-Dependent Transcriptional Regulator AmrZ (AlgZ) Inhibits Flagellum Biosynthesis in Mucoid, Nonmotile Pseudomonas aeruginosa Cystic Fibrosis Isolates. J. Bacteriol. 2006, 188, 6483–6489. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Gicquel, G.; Bouffartigues, E.; Bains, M.; Oxaran, V.; Rosay, T.; Lesouhaitier, O.; Connil, N.; Bazire, A.; Maillot, O.; Bénard, M.; et al. The Extra-Cytoplasmic Function Sigma Factor SigX Modulates Biofilm and Virulence-Related Properties in Pseudomonas aeruginosa. PLoS ONE 2013, 8, e80407. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Brencic, A.; Lory, S. Determination of the Regulon and Identification of Novel MRNA Targets of Pseudomonas aeruginosa RsmA. Mol. Microbiol. 2009, 72, 612–632. [Google Scholar] [CrossRef] [Green Version]
- Hobbs, M.; Collie, E.S.R.; Free, P.D.; Livingston, S.P.; Mattick, J.S. PilS and PilR, a Two-component Transcriptional Regulatory System Controlling Expression of Type 4 Fimbriae in Pseudomonas aeruginosa. Mol. Microbiol. 1993, 7, 669–682. [Google Scholar] [CrossRef]
- O’Callaghan, J.; Jerry Reen, F.; Adams, C.; Casey, P.G.; Gahan, C.G.M.; O’Gara, F. A Novel Host-Responsive Sensor Mediates Virulence and Type III Secretion during Pseudomonas aeruginosa-Host Cell Interactions. Microbiology 2012, 158, 1057–1070. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Petrova, O.E.; Sauer, K. The Novel Two-Component Regulatory System BfiSR Regulates Biofilm Development by Controlling the Small RNA RsmZ through CafA. J. Bacteriol. 2010, 192, 5275–5288. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Huang, H.; Shao, X.; Xie, Y.; Wang, T.; Zhang, Y.; Wang, X.; Deng, X. An Integrated Genomic Regulatory Network of Virulence-Related Transcriptional Factors in Pseudomonas aeruginosa. Nat. Commun. 2019, 10, 2931. [Google Scholar] [CrossRef] [Green Version]
- Kulasekara, H.D.; Ventre, I.; Kulasekara, B.R.; Lazdunski, A.; Filloux, A.; Lory, S. A Novel Two-Component System Controls the Expression of Pseudomonas aeruginosa Fimbrial Cup Genes. Mol. Microbiol. 2005, 55, 368–380. [Google Scholar] [CrossRef]
- Mikkelsen, H.; Hui, K.; Barraud, N.; Filloux, A. The Pathogenicity Island Encoded PvrSR/RcsCB Regulatory Network Controls Biofilm Formation and Dispersal in Pseudomonas aeruginosa PA14. Mol. Microbiol. 2013, 89, 450–463. [Google Scholar] [CrossRef] [Green Version]
- Wang, C.; Chen, W.; Xia, A.; Zhang, R.; Huang, Y.; Yang, S.; Ni, L.; Jin, F. Carbon Starvation Induces the Expression of Pprb-Regulated Genes in Pseudomonas aeruginosa. Appl. Environ. Microbiol. 2019, 85, e01705-19. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Cai, Z.; Yang, F.; Shao, X.; Yue, Z.; Li, Z.; Song, Y.; Pan, X.; Jin, Y.; Cheng, Z.; Ha, U.-H.; et al. ECF Sigma Factor HxuI Is Critical for In Vivo Fitness of Pseudomonas aeruginosa during Infection. Microbiol. Spectr. 2022, 10, e01620-21. [Google Scholar] [CrossRef] [PubMed]
- Bazire, A.; Shioya, K.; Soum-Soutéra, E.; Bouffartigues, E.; Ryder, C.; Guentas-Dombrowsky, L.; Hémery, G.; Linossier, I.; Chevalier, S.; Wozniak, D.J.; et al. The Sigma Factor AlgU Plays a Key Role in Formation of Robust Biofilms by Nonmucoid Pseudomonas aeruginosa. J. Bacteriol. 2010, 192, 3001–3010. [Google Scholar] [CrossRef] [Green Version]
- Déziel, E.; Gopalan, S.; Tampakaki, A.P.; Lépine, F.; Padfield, K.E.; Saucier, M.; Xiao, G.; Rahme, L.G. The Contribution of MvfR to Pseudomonas aeruginosa Pathogenesis and Quorum Sensing Circuitry Regulation: Multiple Quorum Sensing-Regulated Genes are Modulated without Affecting LasRI, RhlRI or the Production of N-Acyl-L-Homoserine Lactones. Mol. Microbiol. 2005, 55, 998–1014. [Google Scholar] [CrossRef] [PubMed]
- McGuffie, B.A.; Vallet-Gely, I.; Dove, S.L. σ Factor and Anti-σ Factor That Control Swarming Motility and Biofilm Formation in Pseudomonas aeruginosa. J. Bacteriol. 2016, 198, 755. [Google Scholar] [CrossRef] [Green Version]
- Suh, S.J.; Silo-Suh, L.; Woods, D.E.; Hassett, D.J.; West, S.E.H.; Ohman, D.E. Effect of RpoS Mutation on the Stress Response and Expression of Virulence Factors in Pseudomonas aeruginosa. J. Bacteriol. 1999, 181, 3890–3897. [Google Scholar] [CrossRef]
- Irie, Y.; Starkey, M.; Edwards, A.N.; Wozniak, D.J.; Romeo, T.; Parsek, M.R. Pseudomonas aeruginosa Biofilm Matrix Polysaccharide Psl Is Regulated Transcriptionally by RpoS and Post-Transcriptionally by RsmA. Mol. Microbiol. 2010, 78, 158–172. [Google Scholar] [CrossRef] [Green Version]
- Williamson, K.S.; Dlakić, M.; Akiyama, T.; Franklin, M.J. The Pseudomonas aeruginosa RpoH (Σ32) Regulon and Its Role in Essential Cellular Functions, Starvation Survival, and Antibiotic Tolerance. Int. J. Mol. Sci. 2023, 24, 1513. [Google Scholar] [CrossRef]
- Baynham, P.J.; Wozniak, D.J. Identification and Characterization of AlgZ, an AlgT-Dependent DNA-Binding Protein Required for Pseudomonas aeruginosa AlgD Transcription. Mol. Microbiol. 1996, 22, 97–108. [Google Scholar] [CrossRef]
- Chand, N.S.; Hung, D.T. The Two-Component Sensor Kinase KinB Acts as a Non-Canonical Switch between Acute and Chronic Infection. Virulence 2011, 2, 553–558. [Google Scholar] [CrossRef] [Green Version]
- Stacey, S.D.; Pritchett, C.L. Pseudomonas aeruginosa AlgU Contributes to Posttranscriptional Activity by Increasing RsmA Expression in a MucA22 Strain. J. Bacteriol. 2016, 198, 1812–1826. [Google Scholar] [CrossRef] [Green Version]
- Petrova, O.E.; Schurr, J.R.; Schurr, M.J.; Sauer, K. Microcolony Formation by the Opportunistic Pathogen Pseudomonas aeruginosa Requires Pyruvate Andpyruvate Fermentation. Mol. Microbiol. 2012, 86, 819–835. [Google Scholar] [CrossRef] [Green Version]
- Carty, N.L.; Layland, N.; Colmer-Hamood, J.A.; Calfee, M.W.; Pesci, E.C.; Hamood, A.N. PtxR Modulates the Expression of QS-Controlled Virulence Factors in the Pseudomonas aeruginosa Strain PAO1. Mol. Microbiol. 2006, 61, 782–794. [Google Scholar] [CrossRef]
- Miranda, S.W.; Asfahl, K.L.; Dandekar, A.A.; Greenberg, E.P. Pseudomonas aeruginosa Quorum Sensing. Adv. Exp. Med. Biol. 2022, 1386, 95–115. [Google Scholar] [CrossRef]
- Bremer, E. Liberate and Grab It, Ingest and Digest It: The GbdR Regulon of the Pathogen Pseudomonas aeruginosa. J. Bacteriol. 2014, 196, 3–6. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Firoved, A.M.; Deretic, V. Microarray Analysis of Global Gene Expression in Mucoid Pseudomonas aeruginosa. J. Bacteriol. 2003, 185, 1071–1081. [Google Scholar] [CrossRef] [Green Version]
- Gilbert, K.B.; Kim, T.H.; Gupta, R.; Greenberg, E.P.; Schuster, M. Global Position Analysis of the Pseudomonas aeruginosa Quorum-Sensing Transcription Factor LasR. Mol. Microbiol. 2009, 73, 1072–1085. [Google Scholar] [CrossRef] [Green Version]
- Gellatly, S.L.; Bains, M.; Breidenstein, E.B.; Strehmel, J.; Reffuveille, F.; Taylor, P.K.; Yeung, A.T.; Overhage, J.; Hancock, R.E. Novel Roles for Two-Component Regulatory Systems in Cytotoxicity and Virulence-Related properties in Pseudomonas aeruginosa. AIMS Microbiol. 2018, 4, 173–191. [Google Scholar] [CrossRef] [PubMed]
- Rumbaugh, K.P.; Griswold, J.A.; Hamood, A.N. Contribution of the Regulatory Gene LasR to the Pathogenesis of Pseudomonas aeruginosa Infection of Burned Mice. J. Burn Care Rehabil. 1999, 20, 42–49. [Google Scholar] [CrossRef] [PubMed]
- Ochsner, U.A.; Wilderman, P.J.; Vasil, A.I.; Vasil, M.L. GeneChip Expression Analysis of the Iron Starvation Response in Pseudomonas aeruginosa: Identification of Novel Pyoverdine Biosynthesis Genes. Mol. Microbiol. 2002, 45, 1277–1287. [Google Scholar] [CrossRef]
- Schulz, S.; Eckweiler, D.; Bielecka, A.; Nicolai, T.; Franke, R.; Dötsch, A.; Hornischer, K.; Bruchmann, S.; Düvel, J.; Häussler, S. Elucidation of Sigma Factor-Associated Networks in Pseudomonas aeruginosa Reveals a Modular Architecture with Limited and Function-Specific Crosstalk. PLoS Pathog. 2015, 11, e1004744. [Google Scholar] [CrossRef] [Green Version]
- Pan, X.; Liang, H.; Zhao, X.; Zhang, Q.; Chen, L.; Yue, Z.; Yin, L.; Jin, Y.; Bai, F.; Cheng, Z.; et al. Regulatory and Structural Mechanisms of PvrA-Mediated Regulation of the PQS Quorum-Sensing System and PHA Biosynthesis in Pseudomonas aeruginosa. Nucleic Acids Res. 2023, 51, 2691–2708. [Google Scholar] [CrossRef]
- Lo, Y.L.; Chen, C.L.; Shen, L.; Chen, Y.C.; Wang, Y.H.; Lee, C.C.; Wang, L.C.; Chuang, C.H.; Janapatla, R.P.; Chiu, C.H.; et al. Characterization of the Role of Global Regulator FliA in the Pathophysiology of Pseudomonas aeruginosa Infection. Res. Microbiol. 2018, 169, 135–144. [Google Scholar] [CrossRef] [PubMed]
- Okino, N.; Ito, M. Molecular Mechanism for Sphingosine-Induced Pseudomonas Ceramidase Expression through the Transcriptional Regulator SphR. Sci. Rep. 2016, 6, 38797. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Urbanowski, M.L.; Lykken, G.L.; Yahr, T.L. A Secreted Regulatory Protein Couples Transcription to the Secretory Activity of the Pseudomonas aeruginosa Type III Secretion System. Proc. Natl. Acad. Sci. USA 2005, 102, 9930–9935. [Google Scholar] [CrossRef]
- Daddaoua, A.; Fillet, S.; Fernández, M.; Udaondo, Z.; Krell, T.; Ramos, J.L. Genes for Carbon Metabolism and the ToxA Virulence Factor in Pseudomonas aeruginosa are Regulated through Molecular Interactions of PtxR and PtxS. PLoS ONE 2012, 7, e39390. [Google Scholar] [CrossRef] [Green Version]
- Daddaoua, A.; Molina-Santiago, C.; De La Torre, J.; Krell, T.; Ramos, J.L. GtrS and GltR Form a Two-Component System: The Central Role of 2-Ketogluconate in the Expression of Exotoxin A and Glucose Catabolic Enzymes in Pseudomonas aeruginosa. Nucleic Acids Res. 2014, 42, 7654–7665. [Google Scholar] [CrossRef] [Green Version]
- Firoved, A.M.; Ornatowski, W.; Deretic, V. Microarray Analysis Reveals Induction of Lipoprotein Genes in Mucoid Pseudomonas aeruginosa: Implications for Inflammation in Cystic Fibrosis. Infect. Immun. 2004, 72, 5012. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Balasubramanian, D.; Kumari, H.; Jaric, M.; Fernandez, M.; Turner, K.H.; Dove, S.L.; Narasimhan, G.; Lory, S.; Mathee, K. Deep Sequencing Analyses Expands the Pseudomonas aeruginosa AmpR Regulon to Include Small RNA-Mediated Regulation of Iron Acquisition, Heat Shock and Oxidative Stress Response. Nucleic Acids Res. 2014, 42, 979. [Google Scholar] [CrossRef] [PubMed]
- Zhao, J.; Yu, X.; Zhu, M.; Kang, H.; Ma, J.; Wu, M.; Gan, J.; Deng, X.; Liang, H. Structural and Molecular Mechanism of CdpR Involved in Quorum-Sensing and Bacterial Virulence in Pseudomonas aeruginosa. PLoS Biol. 2016, 14, e1002449. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Sánchez-Jiménez, A.; Marcos-Torres, F.J.; Llamas, M.A. Mechanisms of Iron Homeostasis in Pseudomonas aeruginosa and Emerging Therapeutics Directed to Disrupt This Vital Process. Microb. Biotechnol. 2023, 16, 1475–1491. [Google Scholar] [CrossRef] [PubMed]
- Visca, P.; Imperi, F. An Essential Transcriptional Regulator: The Case of Pseudomonas aeruginosa Fur. Future Microbiol. 2018, 13, 853–856. [Google Scholar] [CrossRef]
- Galdino, A.C.M.; Branquinha, M.H.; Santos, A.L.S.; Viganor, L. Pseudomonas aeruginosa and Its Arsenal of Proteases: Weapons to Battle the Host. In Pathophysiological Aspects of Proteases; Springer: Berlin/Heidelberg, Germany, 2017; pp. 381–397. [Google Scholar] [CrossRef] [Green Version]
- López, D.J.; Collado, M.I.; Ibarguren, M.; Vasil, A.I.; Vasil, M.L.; Goñi, F.M.; Alonso, A. Multiple Phospholipid Substrates of Phospholipase C/Sphingomyelinase HR2 from Pseudomonas aeruginosa. Chem. Phys. Lipids 2011, 164, 78–82. [Google Scholar] [CrossRef] [PubMed]
- Okino, N.; Ito, M. Ceramidase Enhances Phospholipase C-Induced Hemolysis by Pseudomonas aeruginosa. J. Biol. Chem. 2007, 282, 6021–6030. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Vasil, M.L.; Stonehouse, M.J.; Vasil, A.I.; Wadsworth, S.J.; Goldfine, H.; Bolcome, R.E.; Chan, J. A Complex Extracellular Sphingomyelinase of Pseudomonas aeruginosa Inhibits Angiogenesis by Selective Cytotoxicity to Endothelial Cells. PLoS Pathog. 2009, 5, e1000420. [Google Scholar] [CrossRef] [Green Version]
- Wood, S.J.; Goldufsky, J.W.; Seu, M.Y.; Dorafshar, A.H.; Shafikhani, S.H. Pseudomonas aeruginosa Cytotoxins: Mechanisms of Cytotoxicity and Impact on Inflammatory Responses. Cells 2023, 12, 195. [Google Scholar] [CrossRef]
- Hauser, A.R. The Type III Secretion System of Pseudomonas aeruginosa: Infection by Injection. Nat. Rev. Microbiol. 2009, 7, 654–665. [Google Scholar] [CrossRef] [Green Version]
- Finck-Barbançon, V.; Goranson, J.; Zhu, L.; Sawa, T.; Wiener-Kronish, J.P.; Fleiszig, S.M.J.; Wu, C.; Mende-Mueller, L.; Frank, D.W. ExoU Expression by Pseudomonas aeruginosa Correlates with Acute Cytotoxicity and Epithelial Injury. Mol. Microbiol. 1997, 25, 547–557. [Google Scholar] [CrossRef]
- Roy-Burman, A.; Savel, R.H.; Racine, S.; Swanson, B.L.; Revadigar, N.S.; Fujimoto, J.; Sawa, T.; Frank, D.W.; Wiener-Kronish, J.P. Type III Protein Secretion Is Associated with Death in Lower Respiratory and Systemic Pseudomonas aeruginosa Infections. J. Infect. Dis. 2001, 183, 1767–1774. [Google Scholar] [CrossRef] [Green Version]
- Kroken, A.R.; Kumar, N.G.; Yahr, T.L.; Smith, B.E.; Nieto, V.; Horneman, H.; Evans, D.J.; Fleiszig, S.M.J. Exotoxin S Secreted by Internalized Pseudomonas aeruginosa Delays Lytic Host Cell Death. PLoS Pathog. 2022, 18, e1010306. [Google Scholar] [CrossRef]
- Wedekind, J.E.; Trame, C.B.; Dorywalska, M.; Koehl, P.; Raschke, T.M.; McKee, M.; FitzGerald, D.; Collier, R.J.; McKay, D.B. Refined Crystallographic Structure of Pseudomonas aeruginosa Exotoxin A and Its Implications for the Molecular Mechanism of Toxicity. J. Mol. Biol. 2001, 314, 823–837. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Du, X.; Youle, R.J.; FitzGerald, D.J.; Pastan, I. Pseudomonas Exotoxin A-Mediated Apoptosis Is Bak Dependent and Preceded by the Degradation of Mcl-1. Mol. Cell. Biol. 2010, 30, 3444–3452. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jiang, F.; Waterfield, N.R.; Yang, J.; Yang, G.; Jin, Q. A Pseudomonas aeruginosa Type VI Secretion Phospholipase D Effector Targets Both Prokaryotic and Eukaryotic Cells. Cell Host Microbe 2014, 15, 600–610. [Google Scholar] [CrossRef] [Green Version]
- Sana, T.G.; Berni, B.; Bleves, S. The T6SSs of Pseudomonas aeruginosa Strain PAO1 and Their Effectors: Beyond Bacterial-Cell Targeting. Front. Cell. Infect. Microbiol. 2016, 6, 61. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Sana, T.G.; Baumann, C.; Merdes, A.; Soscia, C.; Rattei, T.; Hachani, A.; Jones, C.; Bennett, K.L.; Filloux, A.; Superti-Furga, G.; et al. Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule Network. MBio 2015, 6, e00712-15. [Google Scholar] [CrossRef] [Green Version]
- Jiang, F.; Wang, X.; Wang, B.; Chen, L.; Zhao, Z.; Waterfield, N.R.; Yang, G.; Jin, Q. The Pseudomonas aeruginosa Type VI Secretion PGAP1-like Effector Induces Host Autophagy by Activating Endoplasmic Reticulum Stress. Cell Rep. 2016, 16, 1502–1509. [Google Scholar] [CrossRef] [Green Version]
- Usher, L.R.; Lawson, R.A.; Geary, I.; Taylor, C.J.; Bingle, C.D.; Taylor, G.W.; Whyte, M.K.B. Induction of Neutrophil Apoptosis by the Pseudomonas aeruginosa Exotoxin Pyocyanin: A Potential Mechanism of Persistent Infection. J. Immunol. 2002, 168, 1861–1868. [Google Scholar] [CrossRef]
- Lau, G.W.; Ran, H.; Kong, F.; Hassett, D.J.; Mavrodi, D. Pseudomonas aeruginosa Pyocyanin Is Critical for Lung Infection in Mice. Infect. Immun. 2004, 72, 4275–4278. [Google Scholar] [CrossRef] [Green Version]
- Anderson, R.D.; Roddam, L.F.; Bettiol, S.; Sanderson, K.; Reid, D.W. Biosignificance of Bacterial Cyanogenesis in the CF Lung. J. Cyst. Fibros. 2010, 9, 158–164. [Google Scholar] [CrossRef] [Green Version]
- Otero-Asman, J.R.; García-García, A.I.; Civantos, C.; Quesada, J.M.; Llamas, M.A. Pseudomonas aeruginosa Possesses Three Distinct Systems for Sensing and Using the Host Molecule Haem. Environ. Microbiol. 2019, 21, 4629–4647. [Google Scholar] [CrossRef]
- Minandri, F.; Imperi, F.; Frangipani, E.; Bonchi, C.; Visaggio, D.; Facchini, M.; Pasquali, P.; Bragonzi, A.; Visca, P. Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection. Infect. Immun. 2016, 84, 2324–2335. [Google Scholar] [CrossRef] [Green Version]
- Kirienko, N.V.; Cezairliyan, B.O.; Ausubel, F.M.; Powell, J.R. Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis Elegans. In Pseudomonas Methods and Protocols; Filloux, A., Ramos, J.-L., Eds.; Springer: New York, NY, USA, 2014; pp. 653–669. ISBN 978-1-4939-0473-0. [Google Scholar]
- Pasqua, M.; Visaggio, D.; Lo Sciuto, A.; Genah, S.; Banin, E.; Visca, P.; Imperi, F. Ferric Uptake Regulator Fur Is Conditionally Essential in Pseudomonas aeruginosa. J. Bacteriol. 2017, 199, e00472-17. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Reinhart, A.A.; Nguyen, A.T.; Brewer, L.K.; Bevere, J.; Jones, J.W.; Kane, M.A.; Damron, F.H.; Barbier, M.; Oglesby-Sherrouse, A.G. The Pseudomonas aeruginosa PrrF Small RNAs Regulate Iron Homeostasis during Acute Murine Lung Infection. Infect. Immun. 2017, 85, e76416. [Google Scholar] [CrossRef] [Green Version]
- Ishihama, A. Functional Modulation of Escherichia coli RNA Polymerase. Annu. Rev. Microbiol. 2000, 54, 499–518. [Google Scholar] [CrossRef] [PubMed]
- Wigneshweraraj, S.; Bose, D.; Burrows, P.C.; Joly, N.; Schumacher, J.; Rappas, M.; Pape, T.; Zhang, X.; Stockley, P.; Severinov, K.; et al. Modus Operandi of the Bacterial RNA Polymerase Containing the Σ54 Promoter-Specificity Factor. Mol. Microbiol. 2008, 68, 538–546. [Google Scholar] [CrossRef] [PubMed]
- Bastiaansen, K.C.; Bitter, W. ECF Sigma Factors: From Stress Management to Iron Uptake. In Bacterial Regulatory Networks; Caister Academic Press: Poole, UK, 2012. [Google Scholar]
- Paget, M.S. Bacterial Sigma Factors and Anti-Sigma Factors: Structure, Function and Distribution. Biomolecules 2015, 5, 1245–1265. [Google Scholar] [CrossRef] [Green Version]
- Browning, D.F.; Busby, S.J.W. Local and Global Regulation of Transcription Initiation in Bacteria. Nat. Rev. Microbiol. 2016, 14, 638–650. [Google Scholar] [CrossRef] [Green Version]
- Ulrich, L.E.; Koonin, E.V.; Zhulin, I.B. One-Component Systems Dominate Signal Transduction in Prokaryotes. Trends Microbiol. 2005, 13, 52–56. [Google Scholar] [CrossRef] [Green Version]
- Gumerov, V.M.; Ortega, D.R.; Adebali, O.; Ulrich, L.E.; Zhulin, I.B. MiST 3.0: An Updated Microbial Signal Transduction Database with an Emphasis on Chemosensory Systems. Nucleic Acids Res. 2020, 48, D459. [Google Scholar] [CrossRef] [Green Version]
- Cashin, P.; Goldsack, L.; Hall, D.; O’Toole, R. Contrasting Signal Transduction Mechanisms in Bacterial and Eukaryotic Gene Transcription. FEMS Microbiol. Lett. 2006, 261, 155–164. [Google Scholar] [CrossRef] [Green Version]
- Lee, J.; Zhang, L. The Hierarchy Quorum Sensing Network in Pseudomonas aeruginosa. Protein Cell 2015, 6, 26–41. [Google Scholar] [CrossRef] [Green Version]
- Rumbaugh, K.P.; Griswold, J.A.; Iglewski, B.H.; Hamood, A.N. Contribution of Quorum Sensing to the Virulence of Pseudomonas aeruginosa in Burn Wound Infections. Infect. Immun. 1999, 67, 5854–5862. [Google Scholar] [CrossRef] [PubMed]
- Kang, H.; Gan, J.; Zhao, J.; Kong, W.; Zhang, J.; Zhu, M.; Li, F.; Song, Y.; Qin, J.; Liang, H. Crystal Structure of Pseudomonas aeruginosa RsaL Bound to Promoter DNA Reaffirms Its Role as a Global Regulator Involved in Quorum-Sensing. Nucleic Acids Res. 2017, 45, 699–710. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Lesprit, P.; Faurisson, F.; Join-Lambert, O.; Roudot-Thoraval, F.; Foglino, M.; Vissuzaine, C.; Carbon, C. Role of the Quorum-Sensing System in Experimental Pneumonia Due to Pseudomonas aeruginosa in Rats. Am. J. Respir. Crit. Care Med. 2003, 167, 1478–1482. [Google Scholar] [CrossRef]
- Ochsner, U.A.; Reiser, J. Autoinducer-Mediated Regulation of Rhamnolipid Biosurfactant Synthesis in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 1995, 92, 6424–6428. [Google Scholar] [CrossRef]
- Mukherjee, S.; Moustafa, D.; Smith, C.D.; Goldberg, J.B.; Bassler, B.L. The RhlR Quorum-Sensing Receptor Controls Pseudomonas aeruginosa Pathogenesis and Biofilm Development Independently of Its Canonical Homoserine Lactone Autoinducer. PLoS Pathog. 2017, 13, e1006504. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Pearson, J.P.; Feldman, M.; Iglewski, B.H.; Prince, A. Pseudomonas aeruginosa Cell-to-Cell Signaling Is Required for Virulence in a Model of Acute Pulmonary Infection. Infect. Immun. 2000, 68, 4331–4334. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- O’Loughlin, C.T.; Miller, L.C.; Siryaporn, A.; Drescher, K.; Semmelhack, M.F.; Bassler, B.L. A Quorum-Sensing Inhibitor Blocks Pseudomonas aeruginosa Virulence and Biofilm Formation. Proc. Natl. Acad. Sci. USA 2013, 110, 17981–17986. [Google Scholar] [CrossRef]
- Diggle, S.P.; Matthijs, S.; Wright, V.J.; Fletcher, M.P.; Chhabra, S.R.; Lamont, I.L.; Kong, X.; Hider, R.C.; Cornelis, P.; Cámara, M.; et al. The Pseudomonas aeruginosa 4-Quinolone Signal Molecules HHQ and PQS Play Multifunctional Roles in Quorum Sensing and Iron Entrapment. Chem. Biol. 2007, 14, 87–96. [Google Scholar] [CrossRef] [Green Version]
- Baynham, P.J.; Ramsey, D.M.; Gvozdyev, B.V.; Cordonnier, E.M.; Wozniak, D.J. The Pseudomonas aeruginosa Ribbon-Helix-Helix DNA-Binding Protein AlgZ (AmrZ) Controls Twitching Motility and Biogenesis of Type IV Pili. J. Bacteriol. 2006, 188, 132–140. [Google Scholar] [CrossRef] [Green Version]
- Jones, C.J.; Newsom, D.; Kelly, B.; Irie, Y.; Jennings, L.K.; Xu, B.; Limoli, D.H.; Harrison, J.J.; Parsek, M.R.; White, P.; et al. ChIP-Seq and RNA-Seq Reveal an AmrZ-Mediated Mechanism for Cyclic Di-GMP Synthesis and Biofilm Development by Pseudomonas aeruginosa. PLoS Pathog. 2014, 10, e1003984. [Google Scholar] [CrossRef] [PubMed]
- Kalia, M.; Resch, M.D.; Cherny, K.E.; Sauer, K. The Alginate and Motility Regulator AmrZ Is Essential for the Regulation of the Dispersion Response by Pseudomonas aeruginosa Biofilms. mSphere 2022, 7, 1–20. [Google Scholar] [CrossRef] [PubMed]
- Gallegos, M.T.; Schleif, R.; Bairoch, A.; Hofmann, K.; Ramos, J.L. Arac/XylS Family of Transcriptional Regulators. Microbiol. Mol. Biol. Rev. 1997, 61, 393–410. [Google Scholar] [CrossRef] [PubMed]
- Tian, Z.; Cheng, S.; Xia, B.; Jin, Y.; Bai, F.; Cheng, Z.; Jin, S.; Liu, X.; Wu, W. Pseudomonas aeruginosa ExsA Regulates a Metalloprotease, ImpA, That Inhibits Phagocytosis of Macrophages. Infect. Immun. 2019, 87, e69519. [Google Scholar] [CrossRef] [PubMed]
- Urbanowski, M.L.; Brutinel, E.D.; Yahr, T.L. Translocation of ExsE into Chinese Hamster Ovary Cells Is Required for Transcriptional Induction of the Pseudomonas aeruginosa Type III Secretion System. Infect. Immun. 2007, 75, 4432–4439. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- McCaw, M.L.; Lykken, G.L.; Singh, P.K.; Yahr, T.L. ExsD Is a Negative Regulator of the Pseudomonas aeruginosa Type III Secretion Regulon. Mol. Microbiol. 2002, 46, 1123–1133. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Karna, S.L.R.; Nguyen, J.Q.; Evani, S.J.; Qian, L.W.; Chen, P.; Abercrombie, J.J.; Sebastian, E.A.; Fourcaudot, A.B.; Leung, K.P. T3SS and Alginate Biosynthesis of Pseudomonas aeruginosa Impair Healing of Infected Rabbit Wounds. Microb. Pathog. 2020, 147, 104254. [Google Scholar] [CrossRef]
- Allsopp, L.P.; Collins, A.C.Z.; Hawkins, E.; Wood, T.E.; Filloux, A. RpoN/Sfa2-Dependent Activation of the Pseudomonas aeruginosa H2-T6SS and Its Cognate Arsenal of Antibacterial Toxins. Nucleic Acids Res. 2022, 50, 227–243. [Google Scholar] [CrossRef]
- Sana, T.G.; Soscia, C.; Tonglet, C.M.; Garvis, S.; Bleves, S. Divergent Control of Two Type VI Secretion Systems by RpoN in Pseudomonas aeruginosa. PLoS ONE 2013, 8, e76030. [Google Scholar] [CrossRef] [Green Version]
- Germán Sánchez, D.; Primo, E.D.; Damiani, M.T.; Lisa, A.T. Pseudomonas aeruginosa GbdR Gene Is Transcribed from a Σ54-Dependent Promoter under the Control of NtrC/CbrB, IHF and BetI. Microbiology 2017, 163, 1343–1354. [Google Scholar] [CrossRef]
- Pan, X.; Fan, Z.; Chen, L.; Liu, C.; Bai, F.; Wei, Y.; Tian, Z.; Dong, Y.; Shi, J.; Chen, H.; et al. PvrA Is a Novel Regulator That Contributes to Pseudomonas aeruginosa Pathogenesis by Controlling Bacterial Utilization of Long Chain Fatty Acids. Nucleic Acids Res. 2021, 48, 5967–5985. [Google Scholar] [CrossRef] [PubMed]
- LaBauve, A.E.; Wargo, M.J. Detection of Host-Derived Sphingosine by Pseudomonas aeruginosa Is Important for Survival in the Murine Lung. PLoS Pathog. 2014, 10, e1003889. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Maddocks, S.E.; Oyston, P.C.F. Structure and Function of the LysR-Type Transcriptional Regulator (LTTR) Family Proteins. Microbiology 2008, 154, 3609–3623. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Daddaoua, A.; Krell, T.; Ramos, J.L. Transcriptional Control by Two Interacting Regulatory Proteins: Identification of the PtxS Binding Site at PtxR. Nucleic Acids Res. 2013, 41, 10150–10156. [Google Scholar] [CrossRef]
- Hobman, J.L. MerR Family Transcription Activators: Similar Designs, Different Specificities. Mol. Microbiol. 2007, 63, 1275–1278. [Google Scholar] [CrossRef]
- Dietrich, L.E.P.; Price-Whelan, A.; Petersen, A.; Whiteley, M.; Newman, D.K. The Phenazine Pyocyanin Is a Terminal Signalling Factor in the Quorum Sensing Network of Pseudomonas aeruginosa. Mol. Microbiol. 2006, 61, 1308–1321. [Google Scholar] [CrossRef]
- Fujikawa, M.; Kobayashi, K.; Tsutsui, Y.; Tanaka, T.; Kozawa, T. Rational Tuning of Superoxide Sensitivity in SoxR, the [2Fe-2S] Transcription Factor: Implications of Species-Specific Lysine Residues. Biochemistry 2017, 56, 403–410. [Google Scholar] [CrossRef]
- Palma, M.; Zurita, J.; Ferreras, J.A.; Worgall, S.; Larone, D.H.; Shi, L.; Campagne, F.; Quadri, L.E.N. Pseudomonas aeruginosa SoxR Does Not Conform to the Archetypal Paradigm for SoxR-Dependent Regulation of the Bacterial Oxidative Stress Adaptive Response. Infect. Immun. 2005, 73, 2958–2966. [Google Scholar] [CrossRef] [Green Version]
- Aendekerk, S.; Ghysels, B.; Cornelis, P.; Baysse, C. Characterization of a New Efflux Pump, MexGHI-OpmD, from Pseudomonas aeruginosa That Confers Resistance to Vanadium. Microbiology 2002, 148, 2371–2381. [Google Scholar] [CrossRef] [Green Version]
- Sakhtah, H.; Koyama, L.; Zhang, Y.; Morales, D.K.; Fields, B.L.; Price-Whelan, A.; Hogan, D.A.; Shepard, K.; Dietrich, L.E.P. The Pseudomonas aeruginosa Efflux Pump MexGHI-OpmD Transports a Natural Phenazine That Controls Gene Expression and Biofilm Development. Proc. Natl. Acad. Sci. USA 2016, 113, E3538–E3547. [Google Scholar] [CrossRef]
- Ha, U.; Jin, S. Expression of the SoxR Gene of Pseudomonas aeruginosa Is Inducible during Infection of Burn Wounds in Mice and Is Required To Cause Efficient Bacteremia. Infect. Immun. 1999, 67, 5324. [Google Scholar] [CrossRef]
- Gifford, D.R.; Furió, V.; Papkou, A.; Vogwill, T.; Oliver, A.; MacLean, R.C. Identifying and Exploiting Genes That Potentiate the Evolution of Antibiotic Resistance. Nat. Ecol. Evol. 2018, 2, 1033–1039. [Google Scholar] [CrossRef]
- Dik, D.A.; Domínguez-Gil, T.; Lee, M.; Hesek, D.; Byun, B.; Fishovitz, J.; Boggess, B.; Hellman, L.M.; Fisher, J.F.; Hermoso, J.A.; et al. Muropeptide Binding and the X-ray Structure of the Effector Domain of the Transcriptional Regulator AmpR of Pseudomonas aeruginosa. J. Am. Chem. Soc. 2017, 139, 1448. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Balasubramanian, D.; Schneper, L.; Merighi, M.; Smith, R.; Narasimhan, G.; Lory, S.; Mathee, K. The Regulatory Repertoire of Pseudomonas aeruginosa AmpC SS-Lactamase Regulator AmpR Includes Virulence Genes. PLoS ONE 2012, 7, e34067. [Google Scholar] [CrossRef] [Green Version]
- Stock, A.M.; Robinson, V.L.; Goudreau, P.N. Two-Component Signal Transduction. Annu. Rev. Biochem. 2000, 69, 183–215. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Groisman, E.A. Feedback Control of Two-Component Regulatory Systems. Annu. Rev. Microbiol. 2016, 70, 103–124. [Google Scholar] [CrossRef] [PubMed]
- Zhou, T.; Huang, J.; Liu, Z.; Lin, Q.; Xu, Z. The Two-Component System FleS/FleR Represses H1-T6SS Via. Appl. Environ. Microbiol. 2022, 88, e01655-21. [Google Scholar] [CrossRef]
- Zhou, T.; Huang, J.; Liu, Z.; Xu, Z.; Zhang, L.H. Molecular Mechanisms Underlying the Regulation of Biofilm Formation and Swimming Motility by FleS/FleR in Pseudomonas aeruginosa. Front. Microbiol. 2021, 12, 707711. [Google Scholar] [CrossRef] [PubMed]
- Colley, B.; Dederer, V.; Carnell, M.; Kjelleberg, S.; Rice, S.A.; Klebensberger, J. SiaA/D Interconnects c-Di-GMP and RsmA Signaling to Coordinate Cellular Aggregation of Pseudomonas aeruginosa in Response to Environmental Conditions. Front. Microbiol. 2016, 7, 179. [Google Scholar] [CrossRef] [Green Version]
- Rao, F.; Yang, Y.; Qi, Y.; Liang, Z.X. Catalytic Mechanism of Cyclic Di-GMP-Specific Phosphodiesterase: A Study of the EAL Domain-Containing RocR from Pseudomonas aeruginosa. J. Bacteriol. 2008, 190, 3622–3631. [Google Scholar] [CrossRef] [Green Version]
- He, J.; Baldini, R.L.; Déziel, E.; Saucier, M.; Zhang, Q.; Liberati, N.T.; Lee, D.; Urbach, J.; Goodman, H.M.; Rahme, L.G. The Broad Host Range Pathogen Pseudomonas aeruginosa Strain PA14 Carries Two Pathogenicity Islands Harboring Plant and Animal Virulence Genes. Proc. Natl. Acad. Sci. USA 2004, 101, 2530–2535. [Google Scholar] [CrossRef]
- Okkotsu, Y.; Tieku, P.; Fitzsimmons, L.F.; Churchill, M.E.; Schurr, M.J. Pseudomonas aeruginosa AlgR Phosphorylation Modulates Rhamnolipid Production and Motility. J. Bacteriol. 2013, 195, 5499–5515. [Google Scholar] [CrossRef] [Green Version]
- Matilla, M.A.; Martín-Mora, D.; Gavira, J.A.; Krell, T. Pseudomonas aeruginosa as a Model To Study Chemosensory Pathway Signaling. Microbiol. Mol. Biol. Rev. 2021, 85, e00151-20. [Google Scholar] [CrossRef]
- Marko, V.A.; Kilmury, S.L.N.; MacNeil, L.T.; Burrows, L.L. Pseudomonas aeruginosa Type IV Minor Pilins and PilY1 Regulate Virulence by Modulating FimS-AlgR Activity. PLoS Pathog. 2018, 14, e1007074. [Google Scholar] [CrossRef] [Green Version]
- Chand, N.S.; Lee, J.S.W.; Clatworthy, A.E.; Golas, A.J.; Smith, R.S.; Hung, D.T. The Sensor Kinase KinB Regulates Virulence in Acute Pseudomonas aeruginosa Infection. J. Bacteriol. 2011, 193, 2989–2999. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Chand, N.S.; Clatworthy, A.E.; Hung, D.T. The Two-Component Sensor KinB Acts as a Phosphatase to Regulate Pseudomonas aeruginosa Virulence. J. Bacteriol. 2012, 194, 6537–6547. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Damron, F.H.; Owings, J.P.; Okkotsu, Y.; Varga, J.J.; Schurr, J.R.; Goldberg, J.B.; Schurr, M.J.; Yu, H.D. Analysis of the Pseudomonas aeruginosa Regulon Controlled by the Sensor Kinase KinB and Sigma Factor RpoN. J. Bacteriol. 2012, 194, 1317–1330. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mikkelsen, H.; McMullan, R.; Filloux, A. The Pseudomonas aeruginosa Reference Strain PA14 Displays Increased Virulence Due to a Mutation in LadS. PLoS ONE 2011, 6, e29113. [Google Scholar] [CrossRef] [Green Version]
- Monteagudo-Cascales, E.; Santero, E.; Canosa, I. The Regulatory Hierarchy Following Signal Integration by the CbrAB Two-Component System: Diversity of Responses and Functions. Genes 2022, 13, 375. [Google Scholar] [CrossRef]
- Ryan Kaler, K.M.; Nix, J.C.; Schubot, F.D. RetS Inhibits Pseudomonas aeruginosa Biofilm Formation by Disrupting the Canonical Histidine Kinase Dimerization Interface of GacS. J. Biol. Chem. 2021, 297, 101193. [Google Scholar] [CrossRef]
- Wang, B.X.; Wheeler, K.M.; Cady, K.C.; Lehoux, S.; Cummings, R.D.; Laub, M.T.; Ribbeck, K. Mucin Glycans Signal through the Sensor Kinase RetS to Inhibit Virulence-Associated Traits in Pseudomonas aeruginosa. Curr. Biol. 2021, 31, 90–102.e7. [Google Scholar] [CrossRef]
- Fadel, F.; Bassim, V.; Francis, V.I.; Porter, S.L.; Botzanowski, T.; Legrand, P.; Perez, M.M.; Bourne, Y.; Cianférani, S.; Vincent, F. Insights into the Atypical Autokinase Activity of the Pseudomonas aeruginosa GacS Histidine Kinase and Its Interaction with RetS. Structure 2022, 30, 1285–1297.e5. [Google Scholar] [CrossRef] [PubMed]
- Petrova, O.E.; Sauer, K. SagS Contributes to the Motile-Sessile Switch and Acts in Concert with BfiSR to Enable Pseudomonas aeruginosa Biofilm Formation. J. Bacteriol. 2011, 193, 6614–6628. [Google Scholar] [CrossRef] [Green Version]
- Cao, Q.; Wang, Y.; Chen, F.; Xia, Y.; Lou, J.; Zhang, X.; Yang, N.; Sun, X.; Zhang, Q.; Zhuo, C.; et al. A Novel Signal Transduction Pathway That Modulates Rhl Quorum Sensing and Bacterial Virulence in Pseudomonas aeruginosa. PLoS Pathog. 2014, 10, e1004340. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Fan, K.; Cao, Q.; Lan, L. Genome-Wide Mapping Reveals Complex Regulatory Activities of Bfmr in Pseudomonas aeruginosa. Microorganisms 2021, 9, 485. [Google Scholar] [CrossRef]
- Sarwar, Z.; Wang, M.X.; Lundgren, B.R.; Nomura, C.T. MifS, a DctB Family Histidine Kinase, Is a Specific Regulator of α-Ketoglutarate Response in Pseudomonas aeruginosa PAO1. Microbiology 2020, 166, 867. [Google Scholar] [CrossRef]
- Xiong, W.; Perna, A.; Jacob, I.B.; Lundgren, B.R.; Wang, G. The Enhancer-Binding Protein MifR, an Essential Regulator of a-Ketoglutarate Transport, Is Required for Full Virulence of Pseudomonas aeruginosa PAO1 in a Mouse Model of Pneumonia. Infect. Immun. 2022, 90, e00136-22. [Google Scholar] [CrossRef]
- Xu, C.; Cao, Q.; Lan, L. Glucose-Binding of Periplasmic Protein Gltb Activates Gtrs-Gltr Two-Component System in Pseudomonas aeruginosa. Microorganisms 2021, 9, 447. [Google Scholar] [CrossRef] [PubMed]
- Quesada, J.M.; Otero-Asman, J.R.; Bastiaansen, K.C.; Civantos, C.; Llamas, M.A. The Activity of the Pseudomonas aeruginosa Virulence Regulator ΣVreI Is Modulated by the Anti-σ Factor VreR and the Transcription Factor PhoB. Front. Microbiol. 2016, 7, 1159. [Google Scholar] [CrossRef] [Green Version]
- Lamarche, M.G.; Wanner, B.L.; Crépin, S.; Harel, J. The Phosphate Regulon and Bacterial Virulence: A Regulatory Network Connecting Phosphate Homeostasis and Pathogenesis. FEMS Microbiol. Rev. 2008, 32, 461–473. [Google Scholar] [CrossRef] [Green Version]
- Ball, G.; Durand, É.; Lazdunski, A.; Filloux, A. A Novel Type II Secretion System in Pseudomonas aeruginosa. Mol. Microbiol. 2002, 43, 475–485. [Google Scholar] [CrossRef]
- Otero-Asman, J.R.; Quesada, J.M.; Jim, K.K.; Ocampo-Sosa, A.; Civantos, C.; Bitter, W.; Llamas, M.A. The Extracytoplasmic Function Sigma Factor ΣVreI Is Active during Infection and Contributes to Phosphate Starvation-Induced Virulence of Pseudomonas aeruginosa. Sci. Rep. 2020, 10, 3139. [Google Scholar] [CrossRef] [Green Version]
- Chekabab, S.M.; Harel, J.; Dozois, C.M. Interplay between Genetic Regulation of Phosphate Homeostasis and Bacterial Virulence. Virulence 2014, 5, 786–793. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhao, X.; Xu, C.; Qu, J.; Jin, Y.; Bai, F.; Cheng, Z.; Wu, W.; Pan, X. PitA Controls the H2- and H3-T6SSs through PhoB in Pseudomonas aeruginosa. Appl. Environ. Microbiol. 2023, 89, e2094-22. [Google Scholar] [CrossRef] [PubMed]
- Dong, Y.; Zhang, X.-F.; An, S.-W.; Xu, J.-L.; Zhang, L.-H. A Novel Two-Component System BqsS-BqsR Modulates Quorum Sensing-Dependent Biofilm Decay in Pseudomonas aeruginosa. Commun. Integr. Biol. 2008, 1, 88–96. [Google Scholar] [CrossRef] [Green Version]
- Schmidberger, A.; Henkel, M.; Hausmann, R.; Schwartz, T. Influence of Ferric Iron on Gene Expression and Rhamnolipid Synthesis during Batch Cultivation of Pseudomonas aeruginosa PAO1. Appl. Microbiol. Biotechnol. 2014, 98, 6725–6737. [Google Scholar] [CrossRef] [PubMed]
- Kreamer, N.N.; Phillips, R.; Newman, D.K.; Boedicker, J.Q. Predicting the Impact of Promoter Variability on Regulatory Outputs. Sci. Rep. 2015, 5, 18238. [Google Scholar] [CrossRef] [PubMed]
- Otero-Asman, J.R.; Wettstadt, S.; Bernal, P.; Llamas, M.A. Diversity of Extracytoplasmic Function Sigma (ΣECF) Factor-Dependent Signaling in Pseudomonas. Mol. Microbiol. 2019, 112, 356–373. [Google Scholar] [CrossRef] [Green Version]
- Bouillet, S.; Ba, M.; Houot, L.; Iobbi-Nivol, C.; Bordi, C. Connected Partner-Switches Control the Life Style of Pseudomonas aeruginosa through RpoS Regulation. Sci. Rep. 2019, 9, 6496. [Google Scholar] [CrossRef] [Green Version]
- Lo, Y.L.; Shen, L.; Chang, C.H.; Bhuwan, M.; Chiu, C.H.; Chang, H.Y. Regulation of Motility and Phenazine Pigment Production by FliA Is Cyclic-Di-Gmp Dependent in Pseudomonas aeruginosa PAO1. PLoS ONE 2016, 11, e155397. [Google Scholar] [CrossRef] [Green Version]
- Ranjani, J.; Sivakumar, R.; Gunasekaran, P.; Velmurugan, G.; Ramasamy, S.; Rajendhran, J. Genome-Wide Identification of Genetic Requirements of Pseudomonas aeruginosa PAO1 for Rat Cardiomyocyte (H9C2) Infection by Insertion Sequencing. Infect. Genet. Evol. 2022, 98, 105231. [Google Scholar] [CrossRef] [PubMed]
- Blanka, A.; Schulz, S.; Eckweiler, D.; Franke, R.; Bielecka, A.; Nicolai, T.; Casilag, F.; Düvel, J.; Abraham, W.R.; Kaever, V.; et al. Identification of the Alternative Sigma Factor SigX Regulon and Its Implications for Pseudomonas aeruginosa Pathogenicity. J. Bacteriol. 2014, 196, 345. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Chevalier, S.; Bouffartigues, E.; Tortuel, D.; David, A.; Tahrioui, A.; Labbé, C.; Barreau, M.; Tareau, A.S.; Louis, M.; Lesouhaitier, O.; et al. Cell Envelope Stress Response in Pseudomonas aeruginosa. In Pseudomonas aeruginosa: Biology, Pathogenesis and Control Strategies; Springer: Berlin/Heidelberg, Germany, 2022; Volume 1386, ISBN 9783031084911. [Google Scholar]
- Wilderman, P.J.; Sowa, N.A.; FitzGerald, D.J.; FitzGerald, P.C.; Gottesman, S.; Ochsner, U.A.; Vasil, M.L. Identification of Tandem Duplicate Regulatory Small RNAs in Pseudomonas aeruginosa Involved in Iron Homeostasis. Proc. Natl. Acad. Sci. USA 2004, 101, 9792–9797. [Google Scholar] [CrossRef] [PubMed]
- Duchesne, R.; Bouffartigues, E.; Oxaran, V.; Maillot, O.; Bénard, M.; Feuilloley, M.G.J.; Orange, N.; Chevalier, S. A Proteomic Approach of SigX Function in Pseudomonas aeruginosa Outer Membrane Composition. J. Proteomics 2013, 94, 451–459. [Google Scholar] [CrossRef]
- Chevalier, S.; Bouffartigues, E.; Bazire, A.; Tahrioui, A.; Duchesne, R.; Tortuel, D.; Maillot, O.; Clamens, T.; Orange, N.; Feuilloley, M.G.J.; et al. Extracytoplasmic Function Sigma Factors in Pseudomonas aeruginosa. Biochim. Biophys. Acta—Gene Regul. Mech. 2019, 1862, 706–721. [Google Scholar] [CrossRef]
- Anthony, M.; Rose, B.; Pegler, M.B.; Elkins, M.; Service, H.; Thamotharampillai, K.; Watson, J.; Robinson, M.; Bye, P.; Merlino, J.; et al. Genetic Analysis of Pseudomonas aeruginosa Isolates from the Sputa of Australian Adult Cystic Fibrosis Patients. J. Clin. Microbiol. 2002, 40, 2772–2778. [Google Scholar] [CrossRef] [Green Version]
- Qaisar, U.; Kruczek, C.J.; Azeem, M.; Javaid, N.; Colmer-Hamood, J.A.; Hamood, A.N. The Pseudomonas aeruginosa Extracellular Secondary Metabolite, Paerucumarin, Chelates Iron and Is Not Localized to Extracellular Membrane Vesicles. J. Microbiol. 2016, 54, 573–581. [Google Scholar] [CrossRef]
- Wood, L.F.; Ohman, D.E. Use of Cell Wall Stress to Characterize Σ22 (AlgT/U) Activation by Regulated Proteolysis and Its Regulon in Pseudomonas aeruginosa. Mol. Microbiol. 2009, 72, 183–201. [Google Scholar] [CrossRef]
- Jones, A.K.; Fulcher, N.B.; Balzer, G.J.; Urbanowski, M.L.; Pritchett, C.L.; Schurr, M.J.; Yahr, T.L.; Wolfgang, M.C. Activation of the Pseudomonas aeruginosa AlgU Regulon through MucA Mutation Inhibits Cyclic AMP/Vfr Signaling. J. Bacteriol. 2010, 192, 5709–5717. [Google Scholar] [CrossRef] [Green Version]
- James, G.A.; Ge Zhao, A.; Usui, M.; Underwood, R.A.; Nguyen, H.; Beyenal, H.; Delancey Pulcini, E.; Agostinho Hunt, A.; Bernstein, H.C.; Fleckman, P.; et al. Microsensor and Transcriptomic Signatures of Oxygen Depletion in Biofilms Associated with Chronic Wounds. Wound Repair Regen. 2016, 24, 373–383. [Google Scholar] [CrossRef]
- Schuster, M.; Hawkins, A.C.; Harwood, C.S.; Greenberg, E.P. The Pseudomonas aeruginosa RpoS Regulon and Its Relationship to Quorum Sensing. Mol. Microbiol. 2004, 51, 973–985. [Google Scholar] [CrossRef] [PubMed]
- Duan, X.; Pan, Y.; Cai, Z.; Liu, Y.; Zhang, Y.; Liu, M.; Liu, Y.; Wang, K.; Zhang, L.; Yang, L. RpoS-Mutation Variants are Selected in Pseudomonas aeruginosa Biofilms under Imipenem Pressure. Cell Biosci. 2021, 11, 138. [Google Scholar] [CrossRef] [PubMed]
- Dong, T.; Schellhorn, H.E. Role of RpoS in Virulence of Pathogens. Infect. Immun. 2010, 78, 887–897. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Wood, L.F.; Leech, A.J.; Ohman, D.E. Cell Wall-Inhibitory Antibiotics Activate the Alginate Biosynthesis Operon in Pseudomonas aeruginosa: Roles of σ22 (AlgT) and the AlgW and Prc Proteases. Mol. Microbiol. 2006, 62, 412–426. [Google Scholar] [CrossRef] [PubMed]
- Llamas, M.A.; Van Der Sar, A.; Chu, B.C.H.; Sparrius, M.; Vogel, H.J.; Bitter, W. A Novel Extracytoplasmic Function (ECF) Sigma Factor Regulates Virulence in Pseudomonas aeruginosa. PLoS Pathog. 2009, 5, e1000572. [Google Scholar] [CrossRef] [Green Version]
- Ball, G.; Viarre, V.; Garvis, S.; Voulhoux, R.; Filloux, A. Type II-Dependent Secretion of a Pseudomonas aeruginosa DING Protein. Res. Microbiol. 2012, 163, 457–469. [Google Scholar] [CrossRef]
- Kang, D.; Kirienko, N.V. High-Throughput Genetic Screen Reveals That Early Attachment and Biofilm Formation are Necessary for Full Pyoverdine Production by Pseudomonas aeruginosa. Front. Microbiol. 2017, 8, e01707. [Google Scholar] [CrossRef]
- Palma, M.; DeLuca, D.; Worgall, S.; Quadri, L.E.N. Transcriptome Analysis of the Response of Pseudomonas aeruginosa to Hydrogen Peroxide. J. Bacteriol. 2004, 186, 248–252. [Google Scholar] [CrossRef] [Green Version]
- Lamont, I.L.; Beare, P.A.; Ochsner, U.; Vasil, A.I.; Vasil, M.L. Siderophore-Mediated Signaling Regulates Virulence Factor Production in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 2002, 99, 7072–7077. [Google Scholar] [CrossRef]
- Imperi, F.; Massai, F.; Facchini, M.; Frangipani, E.; Visaggio, D.; Leoni, L.; Bragonzi, A.; Visca, P. Repurposing the Antimycotic Drug Flucytosine for Suppression of Pseudomonas aeruginosa Pathogenicity. Proc. Natl. Acad. Sci. USA 2013, 110, 7458–7463. [Google Scholar] [CrossRef]
- Llamas, M.A.; Imperi, F.; Visca, P.; Lamont, I.L. Cell-Surface Signaling in Pseudomonas: Stress Responses, Iron Transport, and Pathogenicity. FEMS Microbiol. Rev. 2014, 38, 569–597. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Damron, F.H.; Oglesby-Sherrouse, A.G.; Wilks, A.; Barbier, M. Dual-Seq Transcriptomics Reveals the Battle for Iron during Pseudomonas aeruginosa Acute Murine Pneumonia. Sci. Rep. 2016, 6, 39172. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Turner, K.H.; Everett, J.; Trivedi, U.; Rumbaugh, K.P.; Whiteley, M. Requirements for Pseudomonas aeruginosa Acute Burn and Chronic Surgical Wound Infection. PLoS Genet. 2014, 10, e1004518. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Reig, S.; Le Gouellec, A.; Bleves, S. What Is New in the Anti–Pseudomonas aeruginosa Clinical Development Pipeline Since the 2017 WHO Alert? Front. Cell. Infect. Microbiol. 2022, 12, 862. [Google Scholar] [CrossRef] [PubMed]
Figure 1.
Schematic representation showing the three main classes of transcriptional regulators in P. aeruginosa. Mechanisms underlying gene expression regulation by (a) one-component systems, (b) two-component systems, and (c) alternative σ factors. Positive and negative controls are represented with arrows and T-shaped lines, respectively. OM, outer membrane; CM, cytoplasmic membrane; RIP, regulated intramembrane proteolysis.
Figure 1.
Schematic representation showing the three main classes of transcriptional regulators in P. aeruginosa. Mechanisms underlying gene expression regulation by (a) one-component systems, (b) two-component systems, and (c) alternative σ factors. Positive and negative controls are represented with arrows and T-shaped lines, respectively. OM, outer membrane; CM, cytoplasmic membrane; RIP, regulated intramembrane proteolysis.
Figure 2.
Regulation of virulence by quorum sensing in P. aeruginosa. The Las (depicted in pink), Rhl (depicted in purple), and PQS (depicted in blue) QS systems are governed by the OCS LasR, RhlR, and PqsR, respectively. Upon detection of their signal molecules, each OCS activates the expression of virulence factors and enzymes producing their respective inducing signals. Positive and negative regulations are represented with arrows and T-shaped lines, respectively.
Figure 2.
Regulation of virulence by quorum sensing in P. aeruginosa. The Las (depicted in pink), Rhl (depicted in purple), and PQS (depicted in blue) QS systems are governed by the OCS LasR, RhlR, and PqsR, respectively. Upon detection of their signal molecules, each OCS activates the expression of virulence factors and enzymes producing their respective inducing signals. Positive and negative regulations are represented with arrows and T-shaped lines, respectively.
Figure 3.
Regulation of alginate production and virulence in P. aeruginosa. Alginate production in P. aeruginosa is controlled by the OCS AmrZ (depicted in yellow), the TCSs FimS-AlgR and KinB-AlgB (depicted in purple and green, respectively), and the σAlgT factor (depicted in blue). Positive and negative controls are represented with arrows and T-shaped lines, respectively. OM, outer membrane; CM, cytoplasmic membrane; RIP, regulated intramembrane proteolysis.
Figure 3.
Regulation of alginate production and virulence in P. aeruginosa. Alginate production in P. aeruginosa is controlled by the OCS AmrZ (depicted in yellow), the TCSs FimS-AlgR and KinB-AlgB (depicted in purple and green, respectively), and the σAlgT factor (depicted in blue). Positive and negative controls are represented with arrows and T-shaped lines, respectively. OM, outer membrane; CM, cytoplasmic membrane; RIP, regulated intramembrane proteolysis.
Figure 4.
TCSs governing the switch between a planktonic lifestyle and biofilm formation via RsmA. The global regulator RsmA promotes a planktonic lifestyle by inducing motility and the production of T3SS in P. aeruginosa while blocking EPS production and biofilm formation. RsmA is antagonized by the two sRNAs RsmY and RsmZ. The TCSs GacS-GacR and BfiS-BfiR control RsmA activity by modulating RsmY and RsmZ levels. Positive and negative controls are represented with arrows and T-shaped lines, respectively. OM, outer membrane; CM, cytoplasmic membrane.
Figure 4.
TCSs governing the switch between a planktonic lifestyle and biofilm formation via RsmA. The global regulator RsmA promotes a planktonic lifestyle by inducing motility and the production of T3SS in P. aeruginosa while blocking EPS production and biofilm formation. RsmA is antagonized by the two sRNAs RsmY and RsmZ. The TCSs GacS-GacR and BfiS-BfiR control RsmA activity by modulating RsmY and RsmZ levels. Positive and negative controls are represented with arrows and T-shaped lines, respectively. OM, outer membrane; CM, cytoplasmic membrane.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
MDPI and ACS Style
Sánchez-Jiménez, A.; Llamas, M.A.; Marcos-Torres, F.J. Transcriptional Regulators Controlling Virulence in Pseudomonas aeruginosa. Int. J. Mol. Sci. 2023, 24, 11895. https://doi.org/10.3390/ijms241511895
AMA Style
Sánchez-Jiménez A, Llamas MA, Marcos-Torres FJ. Transcriptional Regulators Controlling Virulence in Pseudomonas aeruginosa. International Journal of Molecular Sciences. 2023; 24(15):11895. https://doi.org/10.3390/ijms241511895
Chicago/Turabian StyleSánchez-Jiménez, Ana, María A. Llamas, and Francisco Javier Marcos-Torres. 2023. "Transcriptional Regulators Controlling Virulence in Pseudomonas aeruginosa" International Journal of Molecular Sciences 24, no. 15: 11895. https://doi.org/10.3390/ijms241511895
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.
