Metabolomics and Transcriptomics Analyses Reveals the Molecular Regulatory Mechanisms of Walnut (Juglans regia L.) Embryos in Response to Shade Treatment
Abstract
:1. Introduction
2. Results
2.1. Effects of Shade Treatment on Environmental and Photosynthetic Parameters
2.2. Analysis of Differentially Expressed Genes (DEGs) in Response to Shade Treatment
2.3. Analysis of Different Accumulated Metabolites (DAMs) in Response to Shade Treatment
2.4. Principal Component Analysis (PCA)
2.5. Kyoto Encyclopedia of Genes and Genomes (KEGG) Enrichment Analysis of Candidate Pathways in Response to Shade Treatment
2.6. Differences in Metabolic Pathways under Varying Shade-Treatment Durations
2.6.1. Lipid Biosynthesis in Response to Shade Treatment
2.6.2. Plant Hormone Signal Transduction in Response to Shade Treatment
2.6.3. Starch and Sucrose Metabolism Processes in Response to Shade Treatment
2.6.4. Glycolysis/Gluconeogenesis in Response to Shade Treatment
2.6.5. Glutathione Metabolism in Response to Shade Treatment
2.6.6. Amino Acid Biosynthesis in Response to Shade Treatment
2.7. Analysis of Transcription Factors (TFs)
2.8. Weighted Gene Co-Expression Network Analysis (WGCNA)
2.9. Expression Validation of RNA-Seq Data by Real-Time PCR (qRT-PCR)
3. Discussion
3.1. Oil Accumulation in Control and Shaded Groups
3.2. Impact of Shade Treatment on Lipid Biosynthesis
3.3. Impact of Shade Treatment on Amino Acid Biosynthesis
3.4. Impact of Shade Treatment on TFs
3.5. Integrated Analysis of Transcriptome and Metabolome Levels
4. Materials and Methods
4.1. Shade Treatment Used in a Walnut Plantation
4.2. Determination of Physiological Indices during the Walnut Fruit-Development Process
4.3. Measurement of Photosynthetic Parameters
4.4. RNA Extraction and Library Construction
4.5. Bioinformatic Analysis of RNA-Seq Data and Co-Expression Network Construction
4.6. Quantitative Analysis
4.7. Metabolite Extraction and LC-MS Analysis
4.8. Metabolomics Data Processing
4.9. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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KEGG Pathway | Compounds | 60 DAP | 90 DAP | 120 DAP |
---|---|---|---|---|
Ratio (S1/CK1) | Ratio (S2/CK2) | Ratio (S3/CK3) | ||
Starch and sucrose metabolism (ko00500) | alpha, alpha’-Trehalose 6-phosphate | 1 | 0.49 | 1 |
Glucose 6-phosphate | 1 | 0.48 | 1 | |
Trehalose | 1 | 0.46 | 1 | |
Biosynthesis of amino acids (ko01230) | L-Aspartic acid | 1 | 9.28 | 2.22 |
2-Oxoglutaric acid | 2.04 | 1 | 1 | |
L-Citrulline | 1 | 2.65 | 8.92 | |
2-Isopropylmalic acid | 1 | 1 | 3.19 | |
L-Tyrosine | 1 | 0.48 | 1 | |
Citric acid | 1 | 2.15 | 1 | |
D-Ribose 5-phosphate | 1 | 0.37 | 1 | |
Pyruvic acid | 1 | 1 | 4.6 | |
Glutamine | 1 | 2.04 | 1 | |
Methionine | 1 | 0.47 | 1 | |
Arginine | 1 | 3.24 | 1 | |
Citrulline | 1 | 2.46 | 5.61 | |
(-)-Riboflavin | 1 | 1 | 0.49 | |
Glutathione metabolism (ko00480) | L-Glutathione | 1 | 0.47 | 0.36 |
Vitamin C | 1 | 0.47 | 1 |
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Liang, M.; Dong, Q.; Zhang, X.; Liu, Y.; Li, H.; Guo, S.; Luan, H.; Jia, P.; Yang, M.; Qi, G. Metabolomics and Transcriptomics Analyses Reveals the Molecular Regulatory Mechanisms of Walnut (Juglans regia L.) Embryos in Response to Shade Treatment. Int. J. Mol. Sci. 2023, 24, 10871. https://doi.org/10.3390/ijms241310871
Liang M, Dong Q, Zhang X, Liu Y, Li H, Guo S, Luan H, Jia P, Yang M, Qi G. Metabolomics and Transcriptomics Analyses Reveals the Molecular Regulatory Mechanisms of Walnut (Juglans regia L.) Embryos in Response to Shade Treatment. International Journal of Molecular Sciences. 2023; 24(13):10871. https://doi.org/10.3390/ijms241310871
Chicago/Turabian StyleLiang, Manman, Qinglong Dong, Xuemei Zhang, Yang Liu, Han Li, Suping Guo, Haoan Luan, Peng Jia, Minsheng Yang, and Guohui Qi. 2023. "Metabolomics and Transcriptomics Analyses Reveals the Molecular Regulatory Mechanisms of Walnut (Juglans regia L.) Embryos in Response to Shade Treatment" International Journal of Molecular Sciences 24, no. 13: 10871. https://doi.org/10.3390/ijms241310871