Activation of β-Adrenoceptors Promotes Lipid Droplet Accumulation in MCF-7 Breast Cancer Cells via cAMP/PKA/EPAC Pathways
Abstract
:1. Introduction
2. Results
2.1. β-Adrenoceptor Activation Triggers LD Expansion in MCF-7 Breast Cancer Cells
2.2. Adrenergic Stimulation Induces Alterations in LD Size and LD Loading Capacity
2.3. Adrenergic-Induced LD Expansion Is Mediated by β1- and β3-Adrenoceptor Activation
2.4. Involvement of PKA and EPAC Pathways in the β1- and β3-Adrenoceptor-Mediated LD Expansion in MCF-7 Breast Cancer Cells
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Cells, Culture Conditions and Treatments
4.3. Nile Red Staining
4.4. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Silva, D.; Kacprzak, K.; Quintas, C.; Gonçalves, J.; Fresco, P. Activation of β-Adrenoceptors Promotes Lipid Droplet Accumulation in MCF-7 Breast Cancer Cells via cAMP/PKA/EPAC Pathways. Int. J. Mol. Sci. 2023, 24, 767. https://doi.org/10.3390/ijms24010767
Silva D, Kacprzak K, Quintas C, Gonçalves J, Fresco P. Activation of β-Adrenoceptors Promotes Lipid Droplet Accumulation in MCF-7 Breast Cancer Cells via cAMP/PKA/EPAC Pathways. International Journal of Molecular Sciences. 2023; 24(1):767. https://doi.org/10.3390/ijms24010767
Chicago/Turabian StyleSilva, Dany, Katarzyna Kacprzak, Clara Quintas, Jorge Gonçalves, and Paula Fresco. 2023. "Activation of β-Adrenoceptors Promotes Lipid Droplet Accumulation in MCF-7 Breast Cancer Cells via cAMP/PKA/EPAC Pathways" International Journal of Molecular Sciences 24, no. 1: 767. https://doi.org/10.3390/ijms24010767
APA StyleSilva, D., Kacprzak, K., Quintas, C., Gonçalves, J., & Fresco, P. (2023). Activation of β-Adrenoceptors Promotes Lipid Droplet Accumulation in MCF-7 Breast Cancer Cells via cAMP/PKA/EPAC Pathways. International Journal of Molecular Sciences, 24(1), 767. https://doi.org/10.3390/ijms24010767