Libanoridin Isolated from Corydalis heterocarpa Inhibits Adipogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells
Abstract
:1. Introduction
2. Results
2.1. Inhibition of Lipid Accumulation
2.2. Supression of PPARγ Expression
2.3. Suppression of Perilipin and Leptin Levels
2.4. Repression of MAPK Activation
3. Materials and Methods
3.1. Materials
3.2. Maintenance and Differentiation of hBM-MSCs
3.3. Assessment of Cytotoxicity
3.4. Oil Red O Staining of the Intracellular Lipid Deposits
3.5. Reverse Transcription Semi-Quantitative Polymerase Chain Reaction (RT-qPCR)
3.6. Immunoblotting
3.7. Immunofluorescence Staining
3.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Karadeniz, F.; Oh, J.H.; Jang, M.S.; Seo, Y.; Kong, C.-S. Libanoridin Isolated from Corydalis heterocarpa Inhibits Adipogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells. Int. J. Mol. Sci. 2023, 24, 254. https://doi.org/10.3390/ijms24010254
Karadeniz F, Oh JH, Jang MS, Seo Y, Kong C-S. Libanoridin Isolated from Corydalis heterocarpa Inhibits Adipogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells. International Journal of Molecular Sciences. 2023; 24(1):254. https://doi.org/10.3390/ijms24010254
Chicago/Turabian StyleKaradeniz, Fatih, Jung Hwan Oh, Mi Soon Jang, Youngwan Seo, and Chang-Suk Kong. 2023. "Libanoridin Isolated from Corydalis heterocarpa Inhibits Adipogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells" International Journal of Molecular Sciences 24, no. 1: 254. https://doi.org/10.3390/ijms24010254