RNase 7 Inhibits Uropathogenic Escherichia coli-Induced Inflammation in Bladder Cells under a High-Glucose Environment by Regulating the JAK/STAT Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. RNase 7 Suppressed the Glucose-Enhanced UPEC Infection in Bladder Epithelial Cells
2.2. Decrease in UPEC Infection in Bladder Epithelial Cells by RNase 7 Was Related to Downregulation of STAT1, STAT3, and TLR4 Expression
2.3. RNase 7 Decreased UPEC Infection-Induced Exogenous Inflammatory Cytokine Secretion
2.4. RNase 7 Suppressed the Enhanced Expression of TLR4 and JAK/STAT Signaling Pathway in Bladder Cells with UPEC Infection in a Concentration-Dependent Manner
2.5. RNase 7 Decreased the UPEC Infection in Bladder Epithelial Cells through Regulation of the JAK/STAT1 Signaling Pathway
2.6. Increased UPEC Infection in Bladder Epithelial Cells in a High-Glucose Environment Was Inhibited by RNase 7 Downregulating the JAK/STAT Pathway-Related Proteins
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Bacterial Strain
4.3. RNase 7 Treatments
4.4. UPEC Infections and Quantifying
4.5. Cytometric Bead Array Immunoassay
4.6. Cell Protein Extraction
4.7. Western Blotting
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ho, C.-H.; Liao, P.-W.; Fan, C.-K.; Liu, S.-P.; Cheng, P.-C. RNase 7 Inhibits Uropathogenic Escherichia coli-Induced Inflammation in Bladder Cells under a High-Glucose Environment by Regulating the JAK/STAT Signaling Pathway. Int. J. Mol. Sci. 2022, 23, 5156. https://doi.org/10.3390/ijms23095156
Ho C-H, Liao P-W, Fan C-K, Liu S-P, Cheng P-C. RNase 7 Inhibits Uropathogenic Escherichia coli-Induced Inflammation in Bladder Cells under a High-Glucose Environment by Regulating the JAK/STAT Signaling Pathway. International Journal of Molecular Sciences. 2022; 23(9):5156. https://doi.org/10.3390/ijms23095156
Chicago/Turabian StyleHo, Chen-Hsun, Pin-Wen Liao, Chia-Kwung Fan, Shih-Ping Liu, and Po-Ching Cheng. 2022. "RNase 7 Inhibits Uropathogenic Escherichia coli-Induced Inflammation in Bladder Cells under a High-Glucose Environment by Regulating the JAK/STAT Signaling Pathway" International Journal of Molecular Sciences 23, no. 9: 5156. https://doi.org/10.3390/ijms23095156