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Article

STN7 Kinase Is Essential for Arabidopsis thaliana Fitness under Prolonged Darkness but Not under Dark-Chilling Conditions

1
Department of Metabolic Regulation, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
2
Department of Plant Anatomy and Cytology, Institute of Plant Experimental Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
3
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Ioannis-Dimosthenis Adamakis
Int. J. Mol. Sci. 2022, 23(9), 4531; https://doi.org/10.3390/ijms23094531
Received: 28 February 2022 / Revised: 11 April 2022 / Accepted: 17 April 2022 / Published: 20 April 2022
(This article belongs to the Special Issue Biotic and Abiotic Stress Effects on Plant Structure and Physiology)
Reversible phosphorylation of photosystem II light harvesting complexes (LHCII) is a well-established protective mechanism enabling efficient response to changing light conditions. However, changes in LHCII phosphorylation were also observed in response to abiotic stress regardless of photoperiod. This study aimed to investigate the impact of dark-chilling on LHCII phosphorylation pattern in chilling-tolerant Arabidopsis thaliana and to check whether the disturbed LHCII phosphorylation process will impact the response of Arabidopsis to the dark-chilling conditions. We analyzed the pattern of LHCII phosphorylation, the organization of chlorophyll–protein complexes, and the level of chilling tolerance by combining biochemical and spectroscopy techniques under dark-chilling and dark conditions in Arabidopsis mutants with disrupted LHCII phosphorylation. Our results show that during dark-chilling, LHCII phosphorylation decreased in all examined plant lines and that no significant differences in dark-chilling response were registered in tested lines. Interestingly, after 24 h of darkness, a high increase in LHCII phosphorylation was observed, co-occurring with a significant FV/FM parameter decrease. The highest drop of FV/FM was detected in the stn7-1 line–mutant, where the LHCII is not phosphorylated, due to the lack of STN7 kinase. Our results imply that STN7 kinase activity is important for mitigating the adverse effects of prolonged darkness. View Full-Text
Keywords: Arabidopsis thaliana; chilling response; darkness; dark-chilling; LHCII phosphorylation; photosynthesis; STN7 kinase Arabidopsis thaliana; chilling response; darkness; dark-chilling; LHCII phosphorylation; photosynthesis; STN7 kinase
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MDPI and ACS Style

Węgrzyn, A.; Krysiak, M.; Kulik, A.; Gieczewska, K.B.; Mazur, R. STN7 Kinase Is Essential for Arabidopsis thaliana Fitness under Prolonged Darkness but Not under Dark-Chilling Conditions. Int. J. Mol. Sci. 2022, 23, 4531. https://doi.org/10.3390/ijms23094531

AMA Style

Węgrzyn A, Krysiak M, Kulik A, Gieczewska KB, Mazur R. STN7 Kinase Is Essential for Arabidopsis thaliana Fitness under Prolonged Darkness but Not under Dark-Chilling Conditions. International Journal of Molecular Sciences. 2022; 23(9):4531. https://doi.org/10.3390/ijms23094531

Chicago/Turabian Style

Węgrzyn, Anna, Małgorzata Krysiak, Anna Kulik, Katarzyna B. Gieczewska, and Radosław Mazur. 2022. "STN7 Kinase Is Essential for Arabidopsis thaliana Fitness under Prolonged Darkness but Not under Dark-Chilling Conditions" International Journal of Molecular Sciences 23, no. 9: 4531. https://doi.org/10.3390/ijms23094531

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