Role of C-Terminal Domain and Membrane Potential in the Mobility of Kv1.3 Channels in Immune Synapse Forming T Cells
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Cells and Cell Culture
4.2. Reagents, Fluorescence Labeling of Cells
4.3. Immunological Synapse (IS) Formation
4.4. Fluorescence Correlation Spectroscopy (FCS)
4.5. Electrophysiology
4.6. Confocal Imaging
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Diffusion Coefficient (µm2/s) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
WT | Non-Conducting | ΔC | ||||||||
IS in | IS out | Standalone | IS in | IS out | Standalone | IS in | IS out | Standalone | ||
Kv1.3 | standard solution | 0.15 ± 0.069 (115) | 0.2 ± 0.11 (98) | 0.086 ± 0.061 (89) | 0.13 ± 0.062 (96) | 0.19 ± 0.079 (91) | 0.056 ± 0.037 (68) | 0.17 ± 0.095 (101) | 0.16 ± 0.073 (91) | 0.25 ± 0.11 (65) |
high K+ solution | 0.09 ± 0.05 (45) | 0.1 ± 0.055 (42) | 0.048 ± 0.022 (35) | 0.12 ± 0.062 (35) | 0.17 ± 0.06 (35) | 0.057 ± 0.037 (25) | 0.12 ± 0.04 (34) | 0.1 ± 0.03 (32) | 0.143 ± 0.063 (23) | |
standard solution | 0.15 ± 0.01 (10) | 0.18 ± 0.09 (13) | 0.07 ± 0.02 (20) | 0.149 ± 0.1 (9) | 0.187 ± 0.09 (13) | 0.04 ± 0.028 (7) | 0.12 ± 0.045 (12) | 0.136 ± 0.016 (7) | 0.21 ± 0.066 (7) | |
MgTx | 0.06 ± 0.03 (13) | 0.09 ± 0.055 (11) | 0.053 ± 0.021 (15) | 0.11 ± 0.08 (12) | 0.15 ± 0.06 (9) | 0.05 ± 0.012 (8) | 0.099 ± 0.03 (14) | 0.097 ± 0.03 (7) | 0.11 ± 0.04 (8) | |
in D10 cells * | 0.021 ± 0.007 (15) | 0.070 ± 0.007 (15) | ||||||||
MHC I | standard solution | 0.34 ± 0.23 (27) | 0.18 ± 0.13 (25) | 0.44 ± 0.31 (24) | ||||||
DiIC18 | standard solution | 0.54 ± 0.38 (19) | 0.68 ± 0.29 (25) | 0.72 ± 0.28 (28) | ||||||
IL-2Rα | standard solution | 0.18 ± 0.09 (30) | 0.25 ± 0.137 (30) | 0.32 ± 0.087 (9) |
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Sebestyén, V.; Nagy, É.; Mocsár, G.; Volkó, J.; Szilágyi, O.; Kenesei, Á.; Panyi, G.; Tóth, K.; Hajdu, P.; Vámosi, G. Role of C-Terminal Domain and Membrane Potential in the Mobility of Kv1.3 Channels in Immune Synapse Forming T Cells. Int. J. Mol. Sci. 2022, 23, 3313. https://doi.org/10.3390/ijms23063313
Sebestyén V, Nagy É, Mocsár G, Volkó J, Szilágyi O, Kenesei Á, Panyi G, Tóth K, Hajdu P, Vámosi G. Role of C-Terminal Domain and Membrane Potential in the Mobility of Kv1.3 Channels in Immune Synapse Forming T Cells. International Journal of Molecular Sciences. 2022; 23(6):3313. https://doi.org/10.3390/ijms23063313
Chicago/Turabian StyleSebestyén, Veronika, Éva Nagy, Gábor Mocsár, Julianna Volkó, Orsolya Szilágyi, Ádám Kenesei, György Panyi, Katalin Tóth, Péter Hajdu, and György Vámosi. 2022. "Role of C-Terminal Domain and Membrane Potential in the Mobility of Kv1.3 Channels in Immune Synapse Forming T Cells" International Journal of Molecular Sciences 23, no. 6: 3313. https://doi.org/10.3390/ijms23063313