Calcium-Dependent Cytosolic Phospholipase A2α as Key Factor in Calcification of Subdermally Implanted Aortic Valve Leaflets
Abstract
:1. Introduction
2. Results
2.1. cPLA2α Expression and iAVL Calcification
2.2. Interleukin-6 (IL-6) Expression
2.3. Expression of OPN and Fetuin-A
2.4. Expression of Cell Death Markers
3. Discussion
3.1. cPLA2α as Key Pro-Calcific Trigger
3.2. Non-Canonical Cell Death in iAVL Calcification
4. Materials and Methods
4.1. Sampling
4.2. Immunohistochemical Assays
4.3. Von Kossa Silver Staining for Calcium Binding Site Detection
4.4. Transmission Electron Microscopy
4.5. Post-Embedding von Kossa Reaction
4.6. Immunogold Labelling
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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nAVL | 2d-iAVL | 7d-iAVL | 14d-iAVL | 28d-iAVL | |
---|---|---|---|---|---|
cPLA2α | ± | ± | ++ | +++ | ++++ |
IL-6 | − | − | −/± | ± | + |
OPN | ± | ± | ++ | +++ | ++++ |
Fetuin-A | ± | − | − | − | − |
Anx-V | ± | ± | + | + | + |
cl-C3 | − | − | − | − | − |
MAP1 | − | − | − | − | − |
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Bonetti, A.; Contin, M.; Tonon, F.; Marchini, M.; Ortolani, F. Calcium-Dependent Cytosolic Phospholipase A2α as Key Factor in Calcification of Subdermally Implanted Aortic Valve Leaflets. Int. J. Mol. Sci. 2022, 23, 1988. https://doi.org/10.3390/ijms23041988
Bonetti A, Contin M, Tonon F, Marchini M, Ortolani F. Calcium-Dependent Cytosolic Phospholipase A2α as Key Factor in Calcification of Subdermally Implanted Aortic Valve Leaflets. International Journal of Molecular Sciences. 2022; 23(4):1988. https://doi.org/10.3390/ijms23041988
Chicago/Turabian StyleBonetti, Antonella, Magali Contin, Federica Tonon, Maurizio Marchini, and Fulvia Ortolani. 2022. "Calcium-Dependent Cytosolic Phospholipase A2α as Key Factor in Calcification of Subdermally Implanted Aortic Valve Leaflets" International Journal of Molecular Sciences 23, no. 4: 1988. https://doi.org/10.3390/ijms23041988