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Article

The Barley Chloroplast Mutator (cpm) Mutant: All Roads Lead to the Msh1 Gene

Instituto de Genética “Ewald A. Favret”, CICVyA (Centro de Investigación en Ciencias Veterinarias y Agronómicas), INTA (Instituto Nacional de Tecnología Agropecuaria), Dr. Nicolás Repetto y de los Reseros s/n, Hurlingham 1686, Argentina
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Author to whom correspondence should be addressed.
Academic Editors: Jean Molinier and Barbara Hohn
Int. J. Mol. Sci. 2022, 23(3), 1814; https://doi.org/10.3390/ijms23031814
Received: 20 September 2021 / Revised: 11 November 2021 / Accepted: 19 November 2021 / Published: 5 February 2022
(This article belongs to the Special Issue DNA Damage and Repair in Plants 2.0)
The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was established in progenies from crosses and backcrosses. These results strongly suggest that the mutation identified in the Msh1 gene of the cpm mutant is responsible for the observed plastome instabilities. Interestingly, comparison of mutant phenotypes and molecular changes induced by the barley cpm mutant with those of Arabidopsis MSH1 mutants revealed marked differences. View Full-Text
Keywords: mismatch repair; barley chloroplast mutator; plastome instability; Msh1 gene mismatch repair; barley chloroplast mutator; plastome instability; Msh1 gene
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MDPI and ACS Style

Lencina, F.; Landau, A.; Prina, A.R. The Barley Chloroplast Mutator (cpm) Mutant: All Roads Lead to the Msh1 Gene. Int. J. Mol. Sci. 2022, 23, 1814. https://doi.org/10.3390/ijms23031814

AMA Style

Lencina F, Landau A, Prina AR. The Barley Chloroplast Mutator (cpm) Mutant: All Roads Lead to the Msh1 Gene. International Journal of Molecular Sciences. 2022; 23(3):1814. https://doi.org/10.3390/ijms23031814

Chicago/Turabian Style

Lencina, Franco, Alejandra Landau, and Alberto R. Prina. 2022. "The Barley Chloroplast Mutator (cpm) Mutant: All Roads Lead to the Msh1 Gene" International Journal of Molecular Sciences 23, no. 3: 1814. https://doi.org/10.3390/ijms23031814

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