Effect of Airborne Particulate Matter on the Immunologic Characteristics of Chronic Rhinosinusitis with Nasal Polyps
Abstract
:1. Introduction
2. Results
2.1. Increased Expression of IL-6, IL-4, IL-33, and CXCL1 on PM10-Treated Human NPDFs
2.2. Cellular Signaling Pathway Elicited by PM10 in Human NPDFs
2.3. Effect of PM10-Treated Human NPDFs on Cytokine Expression of CD4+ T Cells
3. Discussion
4. Materials and Methods
4.1. Isolation of Human NPDF
4.2. Scanning Electron Microscopy
4.3. Z-Stack Confocal Immunofluorescence Analysis
4.4. Quantitative Real-Time PCR
4.5. Western Blot Analysis
4.6. Enzyme-Linked Immunosorbent Assay (ELISA)
4.7. Flow Cytometry, Tonsillar Mononuclear Cells, and CD4+ T Cells
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lee, H.-J.; Kim, D.-K. Effect of Airborne Particulate Matter on the Immunologic Characteristics of Chronic Rhinosinusitis with Nasal Polyps. Int. J. Mol. Sci. 2022, 23, 1018. https://doi.org/10.3390/ijms23031018
Lee H-J, Kim D-K. Effect of Airborne Particulate Matter on the Immunologic Characteristics of Chronic Rhinosinusitis with Nasal Polyps. International Journal of Molecular Sciences. 2022; 23(3):1018. https://doi.org/10.3390/ijms23031018
Chicago/Turabian StyleLee, Hyun-Joo, and Dong-Kyu Kim. 2022. "Effect of Airborne Particulate Matter on the Immunologic Characteristics of Chronic Rhinosinusitis with Nasal Polyps" International Journal of Molecular Sciences 23, no. 3: 1018. https://doi.org/10.3390/ijms23031018