Impact of IBD-Associated Dysbiosis on Bacterial Quorum Sensing Mediated by Acyl-Homoserine Lactone in Human Gut Microbiota
Abstract
:1. Introduction
2. Results
2.1. Presence of AHL in the Gut Microbiota of Controls and IBD Patients
2.2. QS-Related Genes in Human Gut Microbiota
2.2.1. Patients’ Characteristics of the in Silico Study
2.2.2. Microbiota Composition According to Disease Phenotypes
2.2.3. QS-Related Genes
2.2.4. Presence of Homologous QS-Related Genes
2.2.5. AHL Receptor Genes According to Disease Phenotypes
2.2.6. QS-Related Genes According to IBD-Associated Dysbiosis Status
3. Discussion
4. Materials and Methods
4.1. HPLC-MS/MS Samples Pre-Treatment and AHL Extraction
4.2. AHL Detection with HPLC-MS/MS
4.3. Inflammatory Bowel Disease Multi’omics Database and Samples
4.4. Dysbiosis Definitions
4.5. QS-Related Genes
4.6. Sequences Alignment
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Non-IBD n = 26 | CD n = 50 | UC n = 27 | |
---|---|---|---|
Sex (F/M) | 11/15 | 23/27 | 17/10 |
Age (years) (mean +/− SEM) | 29.7 ± 4.0 | 25.9 ± 2.4 | 29.2 ± 3.5 |
Paediatric * (n) | 12 | 26 | 12 |
Disease duration (years) (mean +/− SEM) | 6.0 ± 2.8 | 5.7 ± 1.4 | |
New diagnosis ** (n) | 35 | 14 | |
Antibiotics | 0 | 8 | 1 |
Steroids | 0 | 13 | 2 |
History of surgery | 0 | 10 | 0 |
Clinical activity score (mean +/− SEM) | HBI: 2.1 ± 0.3 Unknown: 4 patients | SCCAI: 1.6 ± 0.2 Unknown: 1 patient |
Accession Number | Function | |
---|---|---|
luxR1 B. fragilis | BF9343_2602 | Putative DNA-binding response regulator |
luxR2 B. fragilis | BF9343_2858 | Putative sigma factor |
luxR3 B. fragilis | BF9343_3797 | Putative LuxR-family regulatory protein |
luxR4 B. fragilis | BF9343_4003 | Putative transcriptional regulator GerE |
luxR1 B. dorei | NZ_LR699004.1:3959839-3960450 | Putative DNA-binding response regulator |
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Grellier, N.; Suzuki, M.T.; Brot, L.; Rodrigues, A.M.S.; Humbert, L.; Escoubeyrou, K.; Rainteau, D.; Grill, J.-P.; Lami, R.; Seksik, P. Impact of IBD-Associated Dysbiosis on Bacterial Quorum Sensing Mediated by Acyl-Homoserine Lactone in Human Gut Microbiota. Int. J. Mol. Sci. 2022, 23, 15404. https://doi.org/10.3390/ijms232315404
Grellier N, Suzuki MT, Brot L, Rodrigues AMS, Humbert L, Escoubeyrou K, Rainteau D, Grill J-P, Lami R, Seksik P. Impact of IBD-Associated Dysbiosis on Bacterial Quorum Sensing Mediated by Acyl-Homoserine Lactone in Human Gut Microbiota. International Journal of Molecular Sciences. 2022; 23(23):15404. https://doi.org/10.3390/ijms232315404
Chicago/Turabian StyleGrellier, Nathan, Marcelino T. Suzuki, Loic Brot, Alice M. S. Rodrigues, Lydie Humbert, Karine Escoubeyrou, Dominique Rainteau, Jean-Pierre Grill, Raphaël Lami, and Philippe Seksik. 2022. "Impact of IBD-Associated Dysbiosis on Bacterial Quorum Sensing Mediated by Acyl-Homoserine Lactone in Human Gut Microbiota" International Journal of Molecular Sciences 23, no. 23: 15404. https://doi.org/10.3390/ijms232315404