Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene
Abstract
:1. Introduction
2. Results
2.1. Phenotypic Analysis
2.2. Transcriptome Sequencing and Transcript Assembly
2.3. Functional Annotation and Classification of Unigenes
2.4. Analysis of DEGs and Candidate Gene Selection
2.5. Validation of Transcriptome Data with qRT-PCR Analysis
2.6. Candidate Gene Cloning and Characterization Analysis
2.7. LoLOB18 Silencing Inhibits Stem Bulblet Formation
3. Discussion
3.1. Different Hormones Regulate Bulblet Formation
3.2. Starch Synthesis Contributes to Bulblet Formation
3.3. LoLOB18 Is Positive Regulator during Underground Bulblet Formation
4. Materials and Methods
4.1. Plant Material and Samples Collection
4.2. RNA Extraction and cDNA Synthesis
4.3. Library Construction and Transcriptome Sequencing
4.4. RNA-Sequencing Data Analysis
4.5. RNA Extraction and qRT-PCR
4.6. Isolation of LoLOB18 Gene
4.7. Virus-Induced Gene Silencing (VIGS)
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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ID | Homologous Gene/Protein | Regulate Pattern (BIII vs. BI) | Regulate Pattern (BII vs. BI) | Log2 FC | |
---|---|---|---|---|---|
BIII vs. BI | BII vs. BI | ||||
Cluster-98963.5096 | SWEET14 | UP | UP | 25.294 | 14.737 |
Cluster-98963.36497 | SWEET14 | UP | UP | 13.14 | 11.302 |
Cluster-95907.0 | HSR201 | DOWN | DOWN | −12.161 | −12.058 |
Cluster-98963.11855 | SLC50A | UP | UP | 12.024 | 14.801 |
Cluster-98963.32942 | SWEET14 | UP | UP | 12.001 | 14.567 |
Cluster-94100.0 | CYP89A2 | DOWN | DOWN | −11.983 | −11.879 |
Cluster-98963.3555 | E2 binding domain | UP | UP | 11.954 | 10.721 |
Cluster-98963.31619 | GAUT12S | UP | UP | 11.589 | 11.511 |
Cluster-98963.30325 | PER64 | UP | UP | 10.841 | 11.787 |
Cluster-39434.0 | LOB18 | UP | UP | 10.258 | 11.872 |
Cluster-98963.3508 | RSI1 | UP | UP | 10.732 | 12.466 |
Cluster-98156.0 | non-specific lipid-transfer protein | UP | UP | 10.667 | 11.265 |
Cluster-89656.0 | PBL28 | UP | UP | 10.59 | 10.543 |
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Fang, S.; Yang, C.; Ali, M.M.; Lin, M.; Tian, S.; Zhang, L.; Chen, F.; Lin, Z. Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene. Int. J. Mol. Sci. 2022, 23, 15246. https://doi.org/10.3390/ijms232315246
Fang S, Yang C, Ali MM, Lin M, Tian S, Zhang L, Chen F, Lin Z. Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene. International Journal of Molecular Sciences. 2022; 23(23):15246. https://doi.org/10.3390/ijms232315246
Chicago/Turabian StyleFang, Shaozhong, Chenglong Yang, Muhammad Moaaz Ali, Mi Lin, Shengnan Tian, Lijuan Zhang, Faxing Chen, and Zhimin Lin. 2022. "Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene" International Journal of Molecular Sciences 23, no. 23: 15246. https://doi.org/10.3390/ijms232315246