Effects of Avenanthramide on the Small Intestinal Damage through Hsp70-NF-κB Signaling in an Ovalbumin-Induced Food Allergy Model
Abstract
:1. Introduction
2. Results
2.1. Effects of AVA on the Allergic and Inflammatory Responses
2.2. Effects of AVA on the Allergic and Inflammatory Responses in the Jejunum
2.3. Effects of AVA on the Small Intestinal Damage Induced by OVA
2.4. Effect of AVA on the Intestinal Hsp70-NF-κB Signaling
2.5. Effect of Hsp70 Activity Inhibition on the Function of AVA in the Allergic Symptoms and the Small Intestinal Damage Induced by OVA
3. Discussion
4. Materials and Methods
4.1. Animal Experiments
4.2. Evaluation of Immunoglobulins and Cytokines
4.3. Western Blot
4.4. Histological Analysis
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Variable | Control | OVA | OVA + LAVA | OVA + HAVA | p Value |
---|---|---|---|---|---|
IL-4 (pg/mL) | 24.87 ± 4.10 d | 48.67 ± 3.82 a | 44.24 ± 3.80 b | 32.76 ± 2.51 c | <0.01 |
TNF-α (pg/mL) | 357.7 ± 36.11 d | 686.7 ± 46.93 a | 567.3 ± 24.48 b | 505.4 ± 37.31 c | <0.01 |
IL-10 (pg/mL) | 40.58 ± 1.78 a | 24.15 ± 2.46 c | 31.09 ± 2.43 b | 33.01 ± 2.25 b | <0.01 |
IL-25 (pg/mL) | 73.09 ± 4.02 a | 44.72 ± 3.87 d | 52.82 ± 5.24 c | 64.42 ± 6.07 b | <0.01 |
IL-33 (pg/mL) | 194.9 ± 9.16 a | 100.6 ± 11.46 d | 120.5 ± 7.12 c | 157.6 ± 16.28 b | <0.01 |
slgA (ng/mL) | 3533 ± 175.3 a | 1804 ± 204.9 d | 2504 ± 263.7 c | 2934 ± 179.6 b | <0.01 |
TSLP (pg/mL) | 41.42 ± 3.65 a | 23.03 ± 4.21 c | 33.20 ± 3.30 b | 36.50 ± 3.54 b | <0.01 |
TGF-β (pg/mL) | 656.9 ± 98.91 a | 233.1 ± 55.31 d | 407.8 ± 44.22 c | 538.8 ± 41.11 b | <0.01 |
Variable | OVA | OVA + HAVA | OVA + HAVA + APO | OVA + APO | p Value |
---|---|---|---|---|---|
IL-4 (pg/mL) | 36.2 ± 3.13 b | 23.23 ± 2.24 d | 31.52 ± 5.52 c | 42.41 ± 3.95 a | <0.01 |
TNF-α (pg/mL) | 519.3 ± 37.91 b | 378.6 ± 27.55 d | 440.5 ± 42.27 c | 628.6 ± 35.15 a | <0.01 |
IL-10 (pg/mL) | 29.33 ± 1.80 b | 35.88 ± 3.17 a | 34.89 ± 1.43 a | 23.58 ± 2.22 c | <0.01 |
IL-25 (pg/mL) | 55.22 ± 4.85 c | 80.37 ± 4.61 a | 69.18 ± 6.26 b | 47.19 ± 4.64 d | <0.01 |
IL-33 (pg/mL) | 123.5 ± 15.74 c | 181.6 ± 13.95 a | 163.3 ± 11.14 b | 106.9 ± 8.07 d | <0.01 |
slgA (ng/mL) | 2173 ± 154.0 c | 3048 ± 232.0 a | 2755 ± 128.8 b | 1901 ± 177.5 d | <0.01 |
TSLP (pg/mL) | 35.86 ± 3.38 | 37.58 ± 4.33 | 37.01 ± 4.16 | 35.03 ± 2.31 | 0.46 |
TGF-β (pg/mL) | 406.9 ± 82.60 c | 690.4 ± 45.14 a | 592 ± 28.36 b | 303.2 ± 32.17 d | <0.01 |
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Liu, P.; Liu, T.; Zhang, M.; Mo, R.; Zhou, W.; Li, D.; Wu, Y. Effects of Avenanthramide on the Small Intestinal Damage through Hsp70-NF-κB Signaling in an Ovalbumin-Induced Food Allergy Model. Int. J. Mol. Sci. 2022, 23, 15229. https://doi.org/10.3390/ijms232315229
Liu P, Liu T, Zhang M, Mo R, Zhou W, Li D, Wu Y. Effects of Avenanthramide on the Small Intestinal Damage through Hsp70-NF-κB Signaling in an Ovalbumin-Induced Food Allergy Model. International Journal of Molecular Sciences. 2022; 23(23):15229. https://doi.org/10.3390/ijms232315229
Chicago/Turabian StyleLiu, Pan, Tianyi Liu, Mingrui Zhang, Ruixia Mo, Weiwei Zhou, Defa Li, and Yi Wu. 2022. "Effects of Avenanthramide on the Small Intestinal Damage through Hsp70-NF-κB Signaling in an Ovalbumin-Induced Food Allergy Model" International Journal of Molecular Sciences 23, no. 23: 15229. https://doi.org/10.3390/ijms232315229