Pregnancy Increases CYP3A Enzymes Activity as Measured by the 4β-Hydroxycholesterol/Cholesterol Ratio
Abstract
:1. Introduction
2. Results
2.1. Baseline Characteristics of the Participants
2.2. Change in the 4β-OHC/Chol Ratio during Pregnancy
2.3. Effect of Pregnancy on the 4β-OHC/Chol Ratio
2.4. Effect of the CYP3A5 Genotype on the 4β-OHC/Chol Ratio
3. Discussion
4. Materials and Methods
4.1. Participant Recruitment, Sample Collection and Processing
4.2. Determination of Plasma 4β-OHC Levels
4.3. Determination of Plasma Cholestrol Levels
4.4. Genotyping
4.5. Data and Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variables | Pregnant Women (n = 110) | Non-Pregnant Women (n = 59) | |
---|---|---|---|
Median age (IQR) (years) | 27 (18 to 33) | 35 (24 to 41) | |
Median weight (IQR) (kg) | 55 (50 to 62) | 65 (54 to 75) | |
Median gestational age (IQR) (weeks) | 22 (19 to 24) | - | |
Gravidity n (%) | Primigravida | 29 (26.4) | - |
Secundigravida | 27 (24.5) | - | |
Multigravida | 54 (49.1) | - |
Variables | N | Median | IQR | Change in Median Value (%) | p-Value | |
---|---|---|---|---|---|---|
4β-OHC (ng/mL) | Second trimester | 110 | 30.25 | 24.62–40.60 | 9.43 | 0.003 |
Third trimester | 110 | 34.80 | 29.30–46.50 | |||
Cholesterol (mmol/L) | Second trimester | 110 | 4.04 | 3.30–5.32 | 2.84 | 0.82 |
Third trimester | 110 | 4.25 | 3.39–5.10 | |||
4β-OHC/Chol × 104 molar ratios | Second trimester | 110 | 0.20 | 0.14–0.25 | 7.31 | 0.02 |
Third trimester | 110 | 0.22 | 0.15–0.32 |
Variable | N | Pregnant Women in Second Trimester | N | Non-Pregnant Women | p-Value | Pregnant Women in Third Trimester | p-Value |
---|---|---|---|---|---|---|---|
Median 4β-OHC (IQR) (ng/mL) | 110 | 30.25 (24.62 to 40.60) | 59 | 23.19 (17.94 to 29.49) | <0.001 | 34.80 (29.30 to 46.50) | <0.001 |
Median Chol (IQR) (mmol/L) | 110 | 4.04 (3.30 to 5.32) | 59 | 4.24 (3.74 to 4.81) | 0.54 | 4.25 (3.39 to 5.10) | 0.96 |
Median 4β-OHC/Chol × 104 molar ratios (IQR) | 110 | 0.20 (0.14 to 0.25) | 59 | 0.14 (0.11 to 0.18) | <0.001 | 0.22 (0.15 to 0.32) | <0.001 |
Geometric mean 4β-OHC/Chol × 104 molar ratios | 110 | 0.20 | 59 | 0.14 | <0.001 | 0.22 | <0.001 |
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Mlugu, E.M.; Minzi, O.M.; Kamuhabwa, A.A.R.; Diczfalusy, U.; Aklillu, E. Pregnancy Increases CYP3A Enzymes Activity as Measured by the 4β-Hydroxycholesterol/Cholesterol Ratio. Int. J. Mol. Sci. 2022, 23, 15168. https://doi.org/10.3390/ijms232315168
Mlugu EM, Minzi OM, Kamuhabwa AAR, Diczfalusy U, Aklillu E. Pregnancy Increases CYP3A Enzymes Activity as Measured by the 4β-Hydroxycholesterol/Cholesterol Ratio. International Journal of Molecular Sciences. 2022; 23(23):15168. https://doi.org/10.3390/ijms232315168
Chicago/Turabian StyleMlugu, Eulambius M., Omary M. Minzi, Appolinary A. R. Kamuhabwa, Ulf Diczfalusy, and Eleni Aklillu. 2022. "Pregnancy Increases CYP3A Enzymes Activity as Measured by the 4β-Hydroxycholesterol/Cholesterol Ratio" International Journal of Molecular Sciences 23, no. 23: 15168. https://doi.org/10.3390/ijms232315168