Inhibition of IRAK1 Is an Effective Therapy for Autoimmune Hypophysitis in Mice
Abstract
:1. Introduction
2. Results
2.1. Higher IRAK1 Was Expressed in the Pituitaries of Mice That Developed EAH
2.2. Rosoxacin Suppressed EAH
2.3. Rosoxacin Inhibited Autoantibody Production
2.4. Rosoxacin Downregulated Production of Cytokines and Chemokines In Vitro
2.5. Rosoxacin Inhibits Class II Major Histocompatibility Complex on Antigen-Presenting Cells In Vitro
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Production and Purification of Recombinant Mouse Growth Hormone (mGH)
4.3. Induction of EAH and Rosoxacin Treatments
4.4. IRAK1 Expression in Pituitaries and Inguinal Lymph Nodes
4.5. Histological Analyses
4.6. Determination of mGH Autoantibody Production
4.7. Multiplex Cytokine Analysis
4.8. Surface Expression of Class II MHC and CD80 on Raw264.7 Cells
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Huang, H.-C.; Chen, Y.-T.; Lin, H.-H.; Li, Z.-Q.; Yang, J.-M.; Tzou, S.-C. Inhibition of IRAK1 Is an Effective Therapy for Autoimmune Hypophysitis in Mice. Int. J. Mol. Sci. 2022, 23, 14958. https://doi.org/10.3390/ijms232314958
Huang H-C, Chen Y-T, Lin H-H, Li Z-Q, Yang J-M, Tzou S-C. Inhibition of IRAK1 Is an Effective Therapy for Autoimmune Hypophysitis in Mice. International Journal of Molecular Sciences. 2022; 23(23):14958. https://doi.org/10.3390/ijms232314958
Chicago/Turabian StyleHuang, Hsiao-Chen, Yun-Ti Chen, Han-Huei Lin, Zhi-Qin Li, Jinn-Moon Yang, and Shey-Cherng Tzou. 2022. "Inhibition of IRAK1 Is an Effective Therapy for Autoimmune Hypophysitis in Mice" International Journal of Molecular Sciences 23, no. 23: 14958. https://doi.org/10.3390/ijms232314958