Treatment with EV-miRNAs Alleviates Obesity-Associated Metabolic Dysfunction in Mice
Abstract
:1. Introduction
2. Results
2.1. Experimental Design, Exogenous EVs’ Biodistribution, and Characterization of Obese Mice
2.2. HIIT and EV-miRNAs Improve the Metabolic Profile of HFD Mice, but Only HIIT Enhances CRF and Promotes Cardiac Remodeling
2.3. HIIT and EV-miRNAs Improve Glucose Tolerance through Different Mechanisms
2.4. Treatment with EV-miRNAs Improves Hepatic Insulin Sensitivity and Steatosis
2.5. HIIT and EV-miRNAs Affect the Hepatic, sWAT, and Muscle Expression Profiles of Obese Mice
2.6. HIIT and EV-miRNAs Partially Reverse the Alterations Induced by Diet
2.7. EV-miRNAs Regulate Hepatic Lipid Metabolism to Decrease Steatosis
3. Discussion
4. Materials and Methods
4.1. Experimental Models
4.2. Indirect Calorimetry
4.3. Echocardiography and Electrocardiogram
4.4. EV Isolation, Characterization, Labeling, and Transfection
4.5. RNA Isolation and Gene Expression Analysis
4.6. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Castaño, C.; Meza-Ramos, A.; Batlle, M.; Guasch, E.; Novials, A.; Párrizas, M. Treatment with EV-miRNAs Alleviates Obesity-Associated Metabolic Dysfunction in Mice. Int. J. Mol. Sci. 2022, 23, 14920. https://doi.org/10.3390/ijms232314920
Castaño C, Meza-Ramos A, Batlle M, Guasch E, Novials A, Párrizas M. Treatment with EV-miRNAs Alleviates Obesity-Associated Metabolic Dysfunction in Mice. International Journal of Molecular Sciences. 2022; 23(23):14920. https://doi.org/10.3390/ijms232314920
Chicago/Turabian StyleCastaño, Carlos, Aline Meza-Ramos, Montserrat Batlle, Eduard Guasch, Anna Novials, and Marcelina Párrizas. 2022. "Treatment with EV-miRNAs Alleviates Obesity-Associated Metabolic Dysfunction in Mice" International Journal of Molecular Sciences 23, no. 23: 14920. https://doi.org/10.3390/ijms232314920