Calix[4]arene Polyamine Triazoles: Synthesis, Aggregation and DNA Binding
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis
2.2. Aggregation Behavior
2.3. CT DNA Binding Abilities
2.3.1. UV Absorption Spectroscopic Study
2.3.2. Fluorescence Spectroscopic Studies
2.3.3. Circular Dichroism Spectral Studies
2.3.4. Particle Size and Zeta Potential Measurements
2.3.5. Transmission Electron Microscopy (TEM)
3. Materials and Methods
3.1. Characterisation Methods
3.2. Reagents
3.3. Sample Preparation
3.4. Instrumentations
3.5. Dynamic and Electrophoretic Light Scattering Study
3.6. UV Absorption Spectroscopic Study
3.7. Steady-State Fluorescence Study
3.8. Circular Dichroism (CD) Study
3.9. Transition Electron Microscopy (TEM) Study
3.10. X-ray Study
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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System | CAC, μM | D Average, nm | d1, nm | d2, nm | PDI | ζ, mV |
---|---|---|---|---|---|---|
5 | - | 221 ± 8 | - | - | 0.256 ± 0.017 | +42 ± 6 |
5 2 | - | 167 ± 32 | - | - | 0.331 ± 0.042 | +46 ± 2 |
5 3 | - | 1679 ± 83 | 2548 ± 79 (78%) | 443 ± 42 (22%) | 0.486 ± 0.040 | −6 ± 1 |
6 | 5.0 | 145 ± 60 | 146 ± 34 (73%) | 10 ± 1 (27%) | 0.339 ± 0.053 | +43 ± 6 |
7 | 3.4 | 19 ± 4 | 16 ± 2 (74%) | 201 ± 72 (26%) | 0.346 ± 0.122 | +48 ± 8 |
Macrocycle | Ksv × 105, M−1 | R2 | Kq × 1013, M−1 | Ka × 105, M−1 | R2 | n |
---|---|---|---|---|---|---|
5 | 0.85 | 0.98 | 0.85 | 0.11 | 0.99 | 0.74 |
6 | 1.29 | 0.99 | 1.29 | 0.17 | 0.99 | 0.83 |
7 | 2.29 | 0.99 | 2.29 | 5.59 | 0.99 | 1.17 |
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Burilov, V.; Makarov, E.; Mironova, D.; Sultanova, E.; Bilyukova, I.; Akyol, K.; Evtugyn, V.; Islamov, D.; Usachev, K.; Mukhametzyanov, T.; et al. Calix[4]arene Polyamine Triazoles: Synthesis, Aggregation and DNA Binding. Int. J. Mol. Sci. 2022, 23, 14889. https://doi.org/10.3390/ijms232314889
Burilov V, Makarov E, Mironova D, Sultanova E, Bilyukova I, Akyol K, Evtugyn V, Islamov D, Usachev K, Mukhametzyanov T, et al. Calix[4]arene Polyamine Triazoles: Synthesis, Aggregation and DNA Binding. International Journal of Molecular Sciences. 2022; 23(23):14889. https://doi.org/10.3390/ijms232314889
Chicago/Turabian StyleBurilov, Vladimir, Egor Makarov, Diana Mironova, Elza Sultanova, Islamiya Bilyukova, Kevser Akyol, Vladimir Evtugyn, Daut Islamov, Konstantin Usachev, Timur Mukhametzyanov, and et al. 2022. "Calix[4]arene Polyamine Triazoles: Synthesis, Aggregation and DNA Binding" International Journal of Molecular Sciences 23, no. 23: 14889. https://doi.org/10.3390/ijms232314889