Tetrabromobisphenol A and Diclazuril Evoke Tissue-Specific Changes of Thyroid Hormone Signaling in Male Thyroid Hormone Action Indicator Mice
Abstract
:1. Introduction
2. Results
2.1. Tissue-Specific Effects of TBBPA and Diclazuril on Peripheral TH Action
2.2. Distinct Impacts of TBBPA and Diclazuril on Local TH Action in Live THAI Mice
2.3. TBBPA Impacts the Hypothalamo–Pituitary–Thyroid (HPT) Axis, While Diclazuril Does Not
2.4. Diclazuril Does Not Impact the Cerebral Cortex; the Effect of TBBPA on dio3 Expression Is Counterbalanced by Local Mechanisms
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Animal Treatment and Sample Collection
4.3. In Vivo Imaging
4.4. Serum Hormone Measurements
4.5. Taqman qPCR
4.6. Cell Transfection and Luciferase Assay
4.7. Data Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Symbol | Gene Name | Assay ID |
---|---|---|
actinb | β actin | Mm02619580_g1 |
dCpG luciferase | dCpG luciferase reporter (custom made) | AIY9ZTZ |
dio3 | deiodinase, iodothyronine type III | Mm00548953_s1 |
gapdh | glyceraldehyde-3-phosphate dehydrogenase | Mm99999915_g1 |
hprt1 | hypoxanthine guanine phosphoribosyl transferase | Mm01545399_m1 |
slc16a2 | MCT8, monocarboxylate transporter 8 | Mm01232724_m1 |
trh | Thyrotropin releasing hormone | Mm01963590_s1 |
tshb | thyroid stimulating hormone, beta subunit | Mm03990915_g1 |
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Sinkó, R.; Rada, K.; Kollár, A.; Mohácsik, P.; Tenk, M.; Fekete, C.; Gereben, B. Tetrabromobisphenol A and Diclazuril Evoke Tissue-Specific Changes of Thyroid Hormone Signaling in Male Thyroid Hormone Action Indicator Mice. Int. J. Mol. Sci. 2022, 23, 14782. https://doi.org/10.3390/ijms232314782
Sinkó R, Rada K, Kollár A, Mohácsik P, Tenk M, Fekete C, Gereben B. Tetrabromobisphenol A and Diclazuril Evoke Tissue-Specific Changes of Thyroid Hormone Signaling in Male Thyroid Hormone Action Indicator Mice. International Journal of Molecular Sciences. 2022; 23(23):14782. https://doi.org/10.3390/ijms232314782
Chicago/Turabian StyleSinkó, Richárd, Kristóf Rada, Anna Kollár, Petra Mohácsik, Miklós Tenk, Csaba Fekete, and Balázs Gereben. 2022. "Tetrabromobisphenol A and Diclazuril Evoke Tissue-Specific Changes of Thyroid Hormone Signaling in Male Thyroid Hormone Action Indicator Mice" International Journal of Molecular Sciences 23, no. 23: 14782. https://doi.org/10.3390/ijms232314782