The L-Rhamnose Biosynthetic Pathway in Trichomonas vaginalis: Identification and Characterization of UDP-D-Glucose 4,6-dehydratase
Abstract
:1. Introduction
2. Results
2.1. Identification of the Genes Involved in the L-Rha Pathway in Tv
2.2. Production of the Recombinant Proteins and Identification of the Enzymatic Activities
2.3. TvUGD Kinetic Properties and Quaternary Structure Characterization
2.4. TvUGD Modeling
2.5. Phylogenetic Analysis of TvUGD
3. Discussion
4. Materials and Methods
4.1. Sequence Identification and Analysis
4.2. Cloning and Expression of the Recombinant Proteins in E. coli
4.3. Analysis of the Enzymatic Activities
4.4. Modeling of TvUGD in Complex with UDP
4.5. Phylogenetic Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Length | Domain(s) | Pfam acc. n. | Predicted Activity | Ortholog in T. Foetus (% id.) |
---|---|---|---|---|---|
TVAG_414560 | 348 aa | PF16363 | 4,6-dehydratase | TRFO_03667 (77%) | |
TVAG_357160 | 285 aa | PF04321 | 4-keto-reductase | ND | |
TVAG_463120 | 450 aa | PF00908/PF04321 | 3,5-epi,4-keto-red | TRFO_30760 (59%) | |
TVAG_016600 | 449 aa | PF00908/PF04321 | 3,5-epi,4-keto-red | TRFO_30760 (59%) | |
TVAG_401850 | 448 aa | PF00908/PF04321 | 3,5-epi,4-keto-red | TRFO_30760 (51%) | |
TVAG_340730 | 347 aa | PF00908 | 3,5-epimerase | TRFO_42104 (37%) | |
TVAG_313960 | 420 aa | PF00908 | 3,5-epimerase | TRFO_42104 (42%) |
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Gaglianone, M.; Laugieri, M.E.; Rojas, A.L.; Coppola, M.R.; Piacente, F.; Fiori, P.L.; Tonetti, M.G. The L-Rhamnose Biosynthetic Pathway in Trichomonas vaginalis: Identification and Characterization of UDP-D-Glucose 4,6-dehydratase. Int. J. Mol. Sci. 2022, 23, 14587. https://doi.org/10.3390/ijms232314587
Gaglianone M, Laugieri ME, Rojas AL, Coppola MR, Piacente F, Fiori PL, Tonetti MG. The L-Rhamnose Biosynthetic Pathway in Trichomonas vaginalis: Identification and Characterization of UDP-D-Glucose 4,6-dehydratase. International Journal of Molecular Sciences. 2022; 23(23):14587. https://doi.org/10.3390/ijms232314587
Chicago/Turabian StyleGaglianone, Matteo, Maria Elena Laugieri, Adriana Lucely Rojas, Maria Rosaria Coppola, Francesco Piacente, Pier Luigi Fiori, and Michela Giulia Tonetti. 2022. "The L-Rhamnose Biosynthetic Pathway in Trichomonas vaginalis: Identification and Characterization of UDP-D-Glucose 4,6-dehydratase" International Journal of Molecular Sciences 23, no. 23: 14587. https://doi.org/10.3390/ijms232314587