Assessment of Bone Microstructure by Micro CT in C57BL/6J Mice for Sex-Specific Differentiation
Abstract
:1. Introduction
2. Results
2.1. Serum Levels of Bone Turnover Markers
2.2. Trabecular Compartment
2.3. Cortical Compartment
2.4. Sex-Specific Discrimination
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Tissue Collection
4.3. Analysis of Bone Microstructure
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Femur | Tibia | Th12 | L5 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Female | Male | p Value | Female | Male | p Value | Female | Male | p Value | Female | Male | p Value | |
BV/TV (%) | 0.162 [0.104; 0.199] | 0.366 [0.285; 0.397] | 0.0009 | 0.141 [0.113; 0.141] | 0.282 [0.223; 0.306] | 0.0040 | 0.362 [0.352; 0.380] | 0.438 [0.423; 0.452] | 0.0060 | 0.327 [0.313; 0.362] | 0.413 [0.388; 0.461] | 0.0060 |
TbN (mm−1) | 3.99 [3.91; 4.03] | 5.44 [5.25; 5.66] | 0.0021 | 3.65 [2.89; 4.01] | 5.43 [5.19; 5.54] | 0.0004 | 5.15 [4.90; 5.34] | 6.41 [6.27; 6.70] | 0.0009 | 4.42 [4.20; 4.62] | 6.08 [5.69; 6.19] | 0.0009 |
TbTh (mm) | 0.073 [0.060; 0.079] | 0.083 [0.073; 0.088] | n.s. | 0.067 [0.063; 0.070] | 0.068 [0.061; 0.069] | n.s. | 0.071 [0.067; 0.072] | 0.067 [0.066; 0.070] | n.s. | 0.070 [0.070; 0.071] | 0.071 [0.064; 0.075] | n.s. |
TbSp (mm) | 0.254 [0.247; 0.281] | 0.176 [0.164; 0.180] | 0.0009 | 0.274 [0.246; 0.358] | 0.171 [0.168; 0.181] | 0.0004 | 0.178 [0.167; 0.187] | 0.132 [0.126; 0.132] | 0.0080 | 0.217 [0.202; 0.233] | 0.147 [0.142; 0.161] | 0.0009 |
TbBMD (mgHA/cm3) | 651.0 [627.4; 686.2] | 686.6 [658.4; 704.5] | n.s. | 728.0 [706.9; 759.7] | 716.1 [703.3; 730.4] | n.s. | 676.2 [670.0; 703.9] | 668.7 [646.0; 680.6] | n.s. | 704.0 [684.6; 710.2] | 698.3 [664.5; 717.3] | n.s. |
Femur | Tibia | Skull | |||||||
---|---|---|---|---|---|---|---|---|---|
Female | Male | p-Value | Female | Male | p-Value | Female | Male | p-Value | |
CtAr/TtAr (%) | 96.85 [96.28; 97.21] | 96.40 [96.66; 96.96] | n.s. | 95.47 [94.32; 96.43] | 96.56 [96.32; 96.84] | n.s. | 82.64 [79.90; 83.84] | 82.27 [80.21; 82.98] | n.s. |
CtTh (mm) | 0.21 [0.21; 0.22] | 0.20 [0.20; 0.20] | 0.0348 | 0.21 [0.20; 0.21] | 0.21 [0.20; 0.22] | n.s. | 0.11 [0.11; 0.12] | 0.12 [0.12; 0.12] | n.s. |
CtPo (%) | 3.2 [2.8; 3.7] | 3.6 [3.0; 4.3] | n.s. | 4.5 [3.6; 5.7] | 3.4 [3.2; 3.7] | n.s. | 17.4 [16.2; 20.1] | 17.7 [17.0; 19.8] | n.s. |
CtBMD (mgHA/cm3) | 992.5 [974.6; 999.1] | 971.2 [949.4; 983.9] | n.s. | 932.7 [904.4; 966.3] | 960.5 [952.5; 967.3] | n.s. | 717.5 [708.6; 725.0] | 721.3 [709.4; 734.1] | n.s. |
AUC—Femur | AUC—Tibia | AUC—Th12 | AUC—L5 | AUC—Skull | |
---|---|---|---|---|---|
Trabecular compartment | |||||
BV/TV (%) | 1.000 | 0.937 | 0.956 | 0.962 | / |
TbN (mm−1) | 1.000 | 1.000 | 0.989 | 1.000 | / |
TbTh (mm) | 0.800 | 0.522 | 0.689 | 0.656 | / |
TbSp (mm) | 1.000 | 1.000 | 0989 | 1.000 | / |
TbBMD (mgHA/cm3) | 0.743 | 0.633 | 0.717 | 0.625 | / |
Cortical compartment | |||||
CtAr/TtAr (%) | 0.729 | 0.839 | / | / | 0.570 |
CtTh (mm) | 0.914 | 0.622 | / | / | 0.675 |
CtPo (%) | 0.729 | 0.839 | / | / | 0.570 |
CtBMD (mgHA/cm3) | 0.771 | 0.711 | / | / | 0.560 |
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Kerschan-Schindl, K.; Papageorgiou, M.; Föger-Samwald, U.; Butylina, M.; Weber, M.; Pietschmann, P. Assessment of Bone Microstructure by Micro CT in C57BL/6J Mice for Sex-Specific Differentiation. Int. J. Mol. Sci. 2022, 23, 14585. https://doi.org/10.3390/ijms232314585
Kerschan-Schindl K, Papageorgiou M, Föger-Samwald U, Butylina M, Weber M, Pietschmann P. Assessment of Bone Microstructure by Micro CT in C57BL/6J Mice for Sex-Specific Differentiation. International Journal of Molecular Sciences. 2022; 23(23):14585. https://doi.org/10.3390/ijms232314585
Chicago/Turabian StyleKerschan-Schindl, Katharina, Maria Papageorgiou, Ursula Föger-Samwald, Maria Butylina, Michael Weber, and Peter Pietschmann. 2022. "Assessment of Bone Microstructure by Micro CT in C57BL/6J Mice for Sex-Specific Differentiation" International Journal of Molecular Sciences 23, no. 23: 14585. https://doi.org/10.3390/ijms232314585