Identification of the Major Effector StSROs in Potato: A Potential StWRKY-SRO6 Regulatory Pathway Enhances Plant Tolerance to Cadmium Stress
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification and Physicochemical Properties of SROs in Potato
2.2. Evolutionary Exploration of SROs in Potato and Different Species
2.3. Phylogenetic Tree Analysis and Classification of SROs in Many Types of Plants
2.4. Gene Structures and Conserved Motif Analysis
2.5. Expression Profile of SROs in Different Potato Tissues and Expression Response Analysis of Five Heavy Metals under Stress
2.6. StSRO Co-Expression Framework
2.7. Prediction of Protein Folding Structure
2.8. StSRO Regulatory Network Analysis
2.9. Effect of Agrobacterium-Mediated Transient Expression of StWRKY6 on Transcription of StSROs 5/6
2.10. In Vitro Culture and Determination of Cd Tolerance of Potato Leaves
2.11. Verification of Cd Tolerance Function of the Heteroexpressed StSRO6 Gene in Yeast Cells
3. Discussion
3.1. Special StSROs 5/6 in the StSRO Family
3.2. OE-StWRKY6 Leads to Co-Expression of StSROs 5/6 to Enhance Potato Cd Tolerance
3.3. Potato MEG (StSRO6) Can Restore Cd Tolerance of Δycf1
3.4. A possible New Pathway for Potato to Cope with Cd Stress
3.5. Conclusions
4. Materials and Methods
4.1. Toxicological Test and Treatment Method
4.2. Identification, Basic Characteristics and Interspecies Collinearity of SRO Family Members
4.3. Interspecies Phylogenetic and Evolutionary Analysis of SROs
4.4. The Functional Analysis of SRO Family Members was Carried out Based on Structures, Motifs, and Cis-Acting Elements
4.5. StSROs under/without Heavy Metal Stress and Co-Expression Network Analysis of Five Heavy Metals
4.6. StSRO Family Interaction Network Analysis and Proteins Tertiary Structure Prediction
4.7. Agrobacterium-Mediated Transient Expression in Potato
4.8. In Vitro Culture Test of Potato Leaf and Determination of Related Enzyme Activity
4.9. PI staining and Cd Localization Staining
4.10. StSRO6 Yeast Functional Complementarity Validation Test
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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He, Y.; He, G.; Lou, F.; Zhou, Z.; Liu, Y.; Zhang, Y.; He, T. Identification of the Major Effector StSROs in Potato: A Potential StWRKY-SRO6 Regulatory Pathway Enhances Plant Tolerance to Cadmium Stress. Int. J. Mol. Sci. 2022, 23, 14318. https://doi.org/10.3390/ijms232214318
He Y, He G, Lou F, Zhou Z, Liu Y, Zhang Y, He T. Identification of the Major Effector StSROs in Potato: A Potential StWRKY-SRO6 Regulatory Pathway Enhances Plant Tolerance to Cadmium Stress. International Journal of Molecular Sciences. 2022; 23(22):14318. https://doi.org/10.3390/ijms232214318
Chicago/Turabian StyleHe, Yeqing, Guandi He, Fei Lou, Zheng Zhou, Yao Liu, Yule Zhang, and Tengbing He. 2022. "Identification of the Major Effector StSROs in Potato: A Potential StWRKY-SRO6 Regulatory Pathway Enhances Plant Tolerance to Cadmium Stress" International Journal of Molecular Sciences 23, no. 22: 14318. https://doi.org/10.3390/ijms232214318