Identification of Novel Nucleocapsid Chimeric Proteins Inhibiting HIV-1 Replication
Abstract
:1. Introduction
2. Results
2.1. The Chimeric Proteins NHT and HTN Have Better Antiviral Effects than HT
2.2. NHT, HTN, and HT Inhibit the Transcription of the HIV-1 Gene in a Tat-Dependent Manner
2.3. NTH, HTN, and HT Interact with and Hijack the Host Cell Factor P-TEFb and Viral RNA by Competing with Tat
2.4. NTH and HTN Interrupt vRNA Packaging and Reduce the Infectivity of the Progeny Virus
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Transfection
4.2. Antibodies
4.3. Preparation of the HIV-1 Virus Stock
4.4. HIV-1 p24 ELISA
4.5. MT4 Cell Infection
4.6. Reporter Gene Assay
4.7. Reverse Transcription Polymerase Chain Reaction (RT-PCR)
4.8. RNA Immunoprecipitation
4.9. Co-Immunoprecipitation
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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LTR Site | Sequence |
---|---|
U3 | tggaagggctaatttggtcccaaaaaagacaagagatccttgatctgtggatctaccacacacaaggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgacctttggatggtgcttcaagttagtaccagttgaaccagagcaagtagaagaggccaaataaggagagaagaacagcttgttacaccctatgagccagcatgggatggaggacccggagggagaagtattagtgtggaagtttgacagcctcctagcatttcgtcacatggcccgagagctgcatccggagtactacaaagactgctgacatcgagctttctacaagggactttccgctggggactttccagggaggtgtggcctgggcgggactggggagtggcgagccctcagatgctacatataagcagctgctttttgcctgtact |
R | gggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgctc |
U5 | aaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagca |
Ψ | gcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcggtattaagcg |
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Kim, H.-I.; Kim, G.-N.; Yu, K.-L.; Park, S.-H.; You, J.C. Identification of Novel Nucleocapsid Chimeric Proteins Inhibiting HIV-1 Replication. Int. J. Mol. Sci. 2022, 23, 12340. https://doi.org/10.3390/ijms232012340
Kim H-I, Kim G-N, Yu K-L, Park S-H, You JC. Identification of Novel Nucleocapsid Chimeric Proteins Inhibiting HIV-1 Replication. International Journal of Molecular Sciences. 2022; 23(20):12340. https://doi.org/10.3390/ijms232012340
Chicago/Turabian StyleKim, Hae-In, Ga-Na Kim, Kyung-Lee Yu, Seong-Hyun Park, and Ji Chang You. 2022. "Identification of Novel Nucleocapsid Chimeric Proteins Inhibiting HIV-1 Replication" International Journal of Molecular Sciences 23, no. 20: 12340. https://doi.org/10.3390/ijms232012340