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Article

Human Amnion-Derived Mesenchymal Stromal Cells: A New Potential Treatment for Carbapenem-Resistant Enterobacterales in Decompensated Cirrhosis

1
Ri.MED Foundation, 90133 Palermo, Italy
2
Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Mediterranean Institute for Transplantation and Advanced Specialized Therapies), 90127 Palermo, Italy
3
Department for the Treatment and Study of Abdominal Disease and Abdominal Transplantation, IRCCS-ISMETT, UPMC, 90127 Palermo, Italy
4
Department of General Surgery and Medical-Surgical Specialties, University of Catania, 95124 Catania, Italy
5
Unit of Infectious Diseases, IRCCS-ISMETT, 90127 Palermo, Italy
*
Author to whom correspondence should be addressed.
Current Address: Nykode Therapeutics AS, Oslo Research Park, Gaustadallèen 21, 0349 Oslo, Norway.
Academic Editor: Francisco J. Vizoso
Int. J. Mol. Sci. 2022, 23(2), 857; https://doi.org/10.3390/ijms23020857
Received: 15 December 2021 / Revised: 5 January 2022 / Accepted: 11 January 2022 / Published: 13 January 2022
Background: Spontaneous bacterial peritonitis (SBP) is a severe and often fatal infection in patients with decompensated cirrhosis and ascites. The only cure for SBP is antibiotic therapy, but the emerging problem of bacterial resistance requires novel therapeutic strategies. Human amniotic mesenchymal stromal cells (hA-MSCs) possess immunomodulatory and anti-inflammatory properties that can be harnessed as a therapy in such a context. Methods: An in vitro applications of hA-MSCs in ascitic fluid (AF) of cirrhotic patients, subsequently infected with carbapenem-resistant Enterobacterales, was performed. We evaluated the effects of hA-MSCs on bacterial load, innate immunity factors, and macrophage phenotypic expression. Results: hA-MSCs added to AF significantly reduce the proliferation of both bacterial strains at 24 h and diversely affect M1 and M2 polarization, C3a complement protein, and ficolin 3 concentrations during the course of infection, in a bacterial strain-dependent fashion. Conclusion: This study shows the potential usefulness of hA-MSC in treating ascites infected with carbapenem-resistant bacteria and lays the foundation to further investigate antibacterial and anti-inflammatory roles of hA-MSC in in vivo models. View Full-Text
Keywords: cirrhosis; ascitic fluid; spontaneous bacterial peritonitis; human amnion-derived mesenchymal stromal cells; carbapenem-resistant Enterobacterales; pattern recognition molecules; ficolins; complement; placenta cirrhosis; ascitic fluid; spontaneous bacterial peritonitis; human amnion-derived mesenchymal stromal cells; carbapenem-resistant Enterobacterales; pattern recognition molecules; ficolins; complement; placenta
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MDPI and ACS Style

Pampalone, M.; Vitale, G.; Gruttadauria, S.; Amico, G.; Iannolo, G.; Douradinha, B.; Mularoni, A.; Conaldi, P.G.; Pietrosi, G. Human Amnion-Derived Mesenchymal Stromal Cells: A New Potential Treatment for Carbapenem-Resistant Enterobacterales in Decompensated Cirrhosis. Int. J. Mol. Sci. 2022, 23, 857. https://doi.org/10.3390/ijms23020857

AMA Style

Pampalone M, Vitale G, Gruttadauria S, Amico G, Iannolo G, Douradinha B, Mularoni A, Conaldi PG, Pietrosi G. Human Amnion-Derived Mesenchymal Stromal Cells: A New Potential Treatment for Carbapenem-Resistant Enterobacterales in Decompensated Cirrhosis. International Journal of Molecular Sciences. 2022; 23(2):857. https://doi.org/10.3390/ijms23020857

Chicago/Turabian Style

Pampalone, Mariangela, Giampiero Vitale, Salvatore Gruttadauria, Giandomenico Amico, Gioacchin Iannolo, Bruno Douradinha, Alessandra Mularoni, Pier G. Conaldi, and Giada Pietrosi. 2022. "Human Amnion-Derived Mesenchymal Stromal Cells: A New Potential Treatment for Carbapenem-Resistant Enterobacterales in Decompensated Cirrhosis" International Journal of Molecular Sciences 23, no. 2: 857. https://doi.org/10.3390/ijms23020857

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