C3H Expression Is Crucial for Methyl Jasmonate Induction of Chicoric Acid Production by Echinacea purpurea (L.) Moench Cell Suspension Cultures
Abstract
:1. Introduction
2. Results
2.1. Evaluation of the Methyl Jasmonate (MeJa) Optimum Concentration to Stimulate Chicoric Acid Biosynthesis in Echinacea Purpurea Cell Suspensions
2.2. Total Polyphenols and Chicoric Acid Content in Echinacea Purpurea Cell Suspensions
2.3. Identification of Gene Sequences Involved in Chicoric Acid Biosynthesis in Echinacea Purpurea and Expression Analysis on Cell Suspensions to Select Markers Elicited by Methyl Jasmonate (MeJa)
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Callus and Cell Suspension Culture Induction
4.3. Cell Growth Time-Points
4.4. Gene Bank Screening and Primer Design
4.5. RNA Extraction and cDNA Synthesis
4.6. Quantitative Real-Time PCR (RT-qPCR)
4.7. UPLC-DAD Analysis of Chicoric Acid and Total Polyphenol Content
5. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Time-Point | Growth Condition | Type of Nutrient Medium |
---|---|---|
T0 | 5 days in the maintenance medium (liquid EP-medium) | Maintenance medium (liquid EP-medium) |
T1 | 7 days in the maintenance medium (liquid EP-medium) | Maintenance medium (liquid EP-medium) |
T2 | 7 days in production medium (EP-F medium) | Production medium (EP-F medium) |
T3 | 9 days in production medium (EP-F medium) | Production medium (EP-F medium) with MeJa for 2 d |
T4 | 12 days in production medium (EP-F medium) | Production medium (EP-F medium) with MeJa for 5 d |
T5 | 14 days in production medium (EP-F medium) | Production medium (EP-F medium) with MeJa for 7 d |
Time from Start of the Analysis (Minutes) | Percentage of Solvent B | Slope |
---|---|---|
0 | 1% | |
1 | 1% | Linear |
11 | 40% | Linear |
12 | 100% | Linear |
13 | 100% | Linear |
13.10 | 1% | Linear |
15 | 1% | Linear |
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Ravazzolo, L.; Ruperti, B.; Frigo, M.; Bertaiola, O.; Pressi, G.; Malagoli, M.; Quaggiotti, S. C3H Expression Is Crucial for Methyl Jasmonate Induction of Chicoric Acid Production by Echinacea purpurea (L.) Moench Cell Suspension Cultures. Int. J. Mol. Sci. 2022, 23, 11179. https://doi.org/10.3390/ijms231911179
Ravazzolo L, Ruperti B, Frigo M, Bertaiola O, Pressi G, Malagoli M, Quaggiotti S. C3H Expression Is Crucial for Methyl Jasmonate Induction of Chicoric Acid Production by Echinacea purpurea (L.) Moench Cell Suspension Cultures. International Journal of Molecular Sciences. 2022; 23(19):11179. https://doi.org/10.3390/ijms231911179
Chicago/Turabian StyleRavazzolo, Laura, Benedetto Ruperti, Marco Frigo, Oriana Bertaiola, Giovanna Pressi, Mario Malagoli, and Silvia Quaggiotti. 2022. "C3H Expression Is Crucial for Methyl Jasmonate Induction of Chicoric Acid Production by Echinacea purpurea (L.) Moench Cell Suspension Cultures" International Journal of Molecular Sciences 23, no. 19: 11179. https://doi.org/10.3390/ijms231911179