Next Article in Journal
The Role of Plant Progesterone in Regulating Growth, Development, and Biotic/Abiotic Stress Responses
Next Article in Special Issue
Genomic Landscape of Mixed-Phenotype Acute Leukemia
Previous Article in Journal
The CFTR Amplifier Nesolicaftor Rescues TGF-β1 Inhibition of Modulator-Corrected F508del CFTR Function
Previous Article in Special Issue
5-Demethylnobiletin Inhibits Cell Proliferation, Downregulates ID1 Expression, Modulates the NF-κB/TNF-α Pathway and Exerts Antileukemic Effects in AML Cells
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 23
Issue 18
10.3390/ijms231810957
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

The Emerging Role of Venetoclax-Based Treatments in Acute Lymphoblastic Leukemia

by
Shlomzion Aumann
,
Adir Shaulov
,
Arnon Haran
,
Noa Gross Even-Zohar
,
Vladimir Vainstein
and
Boaz Nachmias
*
Department of Hematology, Hadassah Medical Center and Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 91120, Israel
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(18), 10957; https://doi.org/10.3390/ijms231810957
Submission received: 30 August 2022 / Revised: 14 September 2022 / Accepted: 16 September 2022 / Published: 19 September 2022
(This article belongs to the Special Issue Cellular and Molecular Mechanisms of Lymphomas and Leukemia)
Browse Figure
Review Reports Versions Notes

Abstract

:
Venetoclax, a B-cell lymphoma (BCL-2) inhibitor, in combination with hypomethylating agents has become the new standard of care in elderly and unfit patients with acute myeloid leukemia, with significantly improved overall survival and quality of life. Studies of venetoclax combined with high-dose chemotherapy are emerging with evidence of higher rates of molecular remission. Recently, a growing number of publications bring forth the use of venetoclax in patients with acute lymphoblastic leukemia (ALL). In the current review, we present the biological rationale of BCL-2 inhibition in ALL, how the interplay of BH3 proteins modulate the response and the current clinical experience with various combinations.

1. Introduction

The B-cell lymphoma (BCL-2) family of proteins are key regulators of the intrinsic cell death pathway. BCL-2-mediated apoptotic signaling features distinct anti-apoptotic proteins (BCL-2, BCL-XL, MCL-1) and pro-apoptotic proteins (BAX, BAK), along with BH3-only proteins, that further regulate these interactions. Following various stimuli, the activation of BH3-only proteins BAD, NOXA, BIM, and BID inhibits BCL-2, thus releasing the inhibition of pro-apoptotic effector molecules BAX and BAK, which leads to the formation of pores within the outer mitochondrial membrane, the release of cytochrome and caspase activation that mediates apoptosis of the cell [1].
Dysregulation of BCL-2 proteins has been demonstrated in many hematological malignancies, including the hallmark translocation t (14; 18) involving BCL-2 in follicular lymphoma, but also in other non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia (CLL) and acute myeloid leukemia (AML) [2,3].
This led to the development of BH3-mimetics that bind BCL-2 and release pro-apoptotic proteins such as BIM, leading to apoptosis induction (Figure 1a). ABT-737 (navitoclax) inhibits BCL-2 as well as BCL-XL and showed preclinical efficacy; however, its clinical use has been limited due to thrombocytopenia, which is mainly related to the concomitant inhibition of BCL-XL [4]. Venetoclax, a more selective BCL-2 inhibitor, has been widely incorporated in the treatment of CLL and AML, changing long-practiced treatment paradigms [5,6,7,8].
Similar to CLL and AML, BCL-2 and BCL-XL overexpression was reported in acute lymphocytic leukemia (ALL), and in recent years, emerging preclinical and clinical reports have highlighted the potential efficacy of venetoclax and other BCL-2 family inhibitors in the treatment of ALL, including T-ALL, B-ALL, TCF3-HLF ALL and MLL-AF4 ALL [9,10,11]. Our growing understanding of the interplay of various BCL-2 family members and BH3-only proteins promotes novel combinations and better-tailored treatment for ALL patients. In this review, we will summarize the current understanding of the biological rationale of targeting specific BCL-2 family proteins in ALL and the current accumulating clinical data.

2. Dysregulation of BCL-2 Proteins in ALL

Overexpression of BCL-2 has been widely reported in ALL. Certain molecular and cytogenetic characteristics have been shown to upregulate BCL-2, suggesting these might be especially sensitive to BCL-2 inhibition. B-ALL with aberrations in mixed-lineage leukemia (MLL) genes, such as t(4; 11), are recognized as a high risk leukemia and often are resistant to conventional therapies, with dismal prognosis [12]. The resulting fusion gene MLL/AF4 was shown to directly bind to the BCL-2 gene and upregulate its expression, whereas other BCL-2 family members, such as MCL1, were not affected [13]. Indeed, t (4; 11) ALL cell lines and primary samples show a higher expression of BCL-2 [14,15]. MLL-rearranged B-ALL cell lines were sensitive to venetoclax in vitro, with resulting apoptosis induction, as compared to wild-type cell lines. Venetoclax was also able to decrease blast counts in the peripheral blood and tumor volume in a mouse xenograft model with these cell lines. Interestingly, disruption of MLL-fusion-driven BCL-2 expression has been proposed as a major mechanism of action for the bromodomain inhibitor I-BET151 [16].
Another well described translocation in ALL is the t (17; 19) translocation, which results in the fusion protein TCF3-HLF and constitutes a subtype of B-ALL with extremely poor prognosis. Among other targets, TCF3-HLF upregulates BCL-2 [17]. Treatment of TCF-HLF-positive B-ALL samples with venetoclax also resulted in the rapid reduction of tumor burden in a mouse xenograft model, linking the elevated expression of BCL-2 and sensitivity to its inhibition [10,18].
Hypodiploid B-ALL is also characterized by the elevated expression of BCL-2 and BIM and low levels of BCL-XL, and were highly sensitive to both navitoclax and venetoclax, suggesting that the balance of BCL-2/BCL-XL expression influences the sensitivity to specific BCL-2 inhibition [19]. Similar results were observed in vivo in mouse xenograft models. Venetoclax treatment induced a rapid reduction in leukemia blasts in peripheral blood, which was durable in 80% of the xenografts. Near-haploid B-ALL showed a more significant response to venetoclax, with all treated mice showing undetectable peripheral blood blasts throughout the 60-day period of the trial. Notably, control Ph+ and Ph-like xenografts did not respond [19]. In accord, studies of Ph+ and Ph-like ALL showed a reduced sensitivity to BCL-2 inhibition compared with Ph- cells [20]. This might be partially explained by a higher BCL-W and lower BCL-2 expression. Endogenous MCL-1, BCL-2 and BCL-XL are expressed in unmanipulated BCR-ABL-transformed B-ALL cells; however, only the loss of MCL-1 resulted in apoptosis induction. Moreover, loss of MCL1 delayed the onset of BCR-ABL-transformed leukemia in a mouse xenograft model, with a strong selection against clones with the MCL-1 deletion [21], suggesting MCL-1 may be a better target for inhibition in Ph+ ALL.

Interplay of Other BH3 Proteins

The correlation between BCL-2 expression and sensitivity to BCL-2 inhibition is not straightforward. BH3 profiling allows us to better identify the dependence of the cell on a specific anti-apoptotic BCL-2 protein, with a possibly better correlation to drug sensitivity [10]. Initial studies with BH3 profiling predicted that ALL cells are dependent on BCL-2 and would be sensitive to its inhibition [22]; however, ALL cells display variability in their sensitivity to BCL-2 inhibition, suggesting other BCL-2 pro- and anti-apoptotic proteins (e.g., BCL-XL, MCL-1) as well as BH3-only proteins (BIM, BAD) modulate the response.
MCL-1. As pro-apoptotic molecules are released upon BCL-2 inhibition, other proteins such as MCL-1 potentially sequester them and prevent apoptosis. Accumulating evidence suggests that navitoclax-induced apoptosis requires the release of BIM and its binding and neutralization of MCL-1 [23]. Immunoprecipitation of BIM in cells treated with navitoclax showed a decrease in binding of BCL-2 and BCL-XL, and an increase in MCL1 interaction [21]. Low levels of MCL-1 were correlated with sensitivity to venetoclax and navitoclax [24], whereas the exogenous expression of MCL-1, including in primary ALL cells, reduced the sensitivity of the cells to navitoclax and rendered venetoclax ineffective [14]. Interestingly, treatment with venetoclax was shown to induce PI3K/AKT/mTOR activation and the upregulation of MCL1 and BCL-XL levels as a possible mechanism of venetoclax resistance [25]. Thus, the balance of these proteins determines the sensitivity to apoptosis induction and can rationalize drug combinations to tip the balance toward apoptosis of the malignant cell.
BCL-XL. BCL-XL has also been shown to correlate with sensitivity to BCL-2 inhibition, with high BCL-2/low BCL-XL having the best response to venetoclax in vivo [26]. Navitoclax, which also inhibits BCL-XL, had a broader activity against the ALL xenograft than venetoclax, although there was a correlation between the response to the two drugs in individual xenografts, suggesting BCL-2 inhibition also contributes to the cytotoxic effect. Chonghaile et al. used BH-3 profiling in various T-ALL cell lines and primary cells and showed a dependence on BCL-XL, with a higher sensitivity to navitoclax that inhibits BCL-XL and BCL-2 compared to the more selective venetoclax. Interestingly, early T-cell progenitor (ETP)-ALL was found to be more BCL-2 dependent. This finding is in correlation with the pattern of BCL-2/BCL-XL expression alongside T-cell maturation. Early progenitor double-negative CD4/CD8 T-cells express abundant BCL-2 and little BCL-XL, whereas double-positive cells express higher levels of BCL-XL. In accordance, patient-derived xenografts with ETP T-ALL cells were sensitive to both venetoclax and navitoclax, whereas the typical T-ALL xenografts were more sensitive to navitoclax [27].
BIM. Venetoclax binds to BCL-2, releasing BIM which recruits BAX/BAK in active conformation to the mitochondrial membrane. BAX/BAK homo-oligomerization leads to mitochondrial outer membrane permeabilization, cytochrome C release and the induction of caspase-mediated apoptosis. BIM was also shown to neutralize MCL-1, reducing another anti-apoptotic barrier. Thus, low BIM levels might render BCL-2 inhibition ineffective. Indeed, lower levels of BIM in Ph+ and Ph-like leukemia cells correlate in vivo with resistance to venetoclax and the knockout of BIM-induced resistance to venetoclax [19].

3. Rationale for Drug Combinations with BCL-2 Inhibition

Modulating BCL-2 family proteins. As elaborated above, MCL-1, BCL-XL and BIM levels can alter the response to BCL-2 inhibition. Studies of concomitant inhibition or modulation of protein levels demonstrate a synergistic effect (Figure 1b). Combining S63845, a selective inhibitor of MCL-1, with venetoclax had a strong synergistic effect in apoptosis induction in multiple T-ALL cell lines in vitro and in vivo [28]. Similarly, silmitasertib, a potent oral inhibitor of casein kinase 2 that reduces MCL-1 levels through enhanced proteosome degradation, showed a synergistic apoptosis induction combined with venetoclax [29]. Combined BCL-2 and MCL-1 inhibition also demonstrated a potent activity in Ph+ ALL xenografts, with a similar efficacy to dasatinib, a tyrosine kinase inhibitor. The combination was also effective in the Ph-like CRLF2-rearranged subtype of B-ALL, suggesting a similar biological phenotype [29,30]. Treatment of Ph+ ALL with the tyrosine kinase inhibitor (TKI) resulted in a decrease in MCL-1 expression, whereas BCL-2 and BCL- XL were not affected. In accord, TKI combined with navitoclax or venetoclax showed a synergistic effect with increased apoptosis and reduced clonogenicity, whereas the depletion of BIM rendered the cells partially resistant to TKI, again demonstrating its possible role in neutralizing MCL-1 [21,31,32]. Dasatinib and ponatinib were also shown to induce the Lck/YES novel tyrosine kinase (LYN), which upregulates BIM and inhibits MCL-1, possibly contributing to the sensitivity to venetoclax [33] and further supporting TKI-BCL-2 inhibition combinations.
BET bromodomain inhibition also affects BIM through the induction of BIM expression and reduced BCL-2 expression, with a resulting synergistic effect from venetoclax and the BET bromodomain inhibitor JO1 in T-ALL cell lines and the patient-derived xenograft [34].
Finally, the co-inhibition of BCL-2 and BCL-XL with a selective inhibitor (A-1155463), or by combining venetoclax and navitoclax, also resulted in the synergistic killing of ALL cells [26].
Chemotherapy-based combinations. BCL-2 silencing experiments, as well as results from preclinical xenograft models, suggest that BCL-2 promotes chemoresistance and cell survival; however, it does not act as a key driver oncogene, and thus, venetoclax might not be effective as monotherapy, but rather more effective in combination with other apoptosis inducers. Indeed, combining venetoclax and chemotherapy showed a prominent synergistic effect [13], resonating with the clinical data presented below. In addition, early reports have shown navitoclax can enhance the cytotoxicity in combination with L-asparaginase, vincristine and dexamethasone, which was observed in cell lines and xenograft models [35].

4. Clinical Experience with Venetoclax-Based Combinations

Based on the preclinical evidence of lymphoblastic cells’ dependence on BCL-2 and BCL-XL, several groups investigated the use of BH3-mimetics in ALL. A summary of significant clinical trials is presented in Table 1.

4.1. Venetoclax with Chemotherapy

The first report of clinical response to venetoclax in ALL was published by Numen et al. in 2018. The patient, a 71 yo female, was diagnosed with early T-cell precursor (ETP)-ALL; she received four treatment lines (hyper-CVAD, nelarabine, L (liposomal)-vincristine, and palbociclib with dexamethasone in a clinical trial) but was refractory. She was then started on mini-CVD (hyper-CVAD minus doxorubicin, with alternating methotrexate and cytarabine) in combination with venetoclax 400 mg. The patient achieved morphological complete remission (CR) after the second cycle with positive minimal residual disease (MRD). At the time of report, the patient was still in remission. The authors also presented a 75 yo patient with non-ETP T-ALL, who was treated with the same regimen and achieved morphologic CR with positive MRD, but his response was transient, with a full-blown relapse 3 weeks after the third cycle. They showed a strong BCL-2 expression in the first patient’s blasts, but not in the second patient’s blasts [36]. Based on this report and the preclinical data of ETP subtype susceptibility to BCL-2 inhibition, a phase I clinical trial testing the combination of venetoclax and mini-CVD in adults with Ph-neg B- and T- (including ETP) ALL was initiated at the Dana-Farber Cancer Institute and MD Anderson Cancer Center. The results were presented in the 2019 ASH annual meeting and included two cohorts: 10 previously untreated older (>60) patients, and 8 patients 18 and older with relapsed or refractory disease (r/r). Venetoclax was administered on days 1 to 21 of a 28-day cycle at two dose levels of 400 mg (3 patients) and 600 mg (15 patients), concurrent with mini-CVD, and followed by allogeneic stem cell transplantation (allo-SCT) or venetoclax + POMP (6-mercaptopurine, vincristine, methotrexate and prednisolone) maintenance. All the previously untreated patients responded to therapy, nine achieved CR with MRD negativity and one patient achieved a partial response (PR). Four patients remained on treatment whereas six patients had allo-SCT. With a median follow up of 11.3 months, none of the patients relapsed until the results were published. Among the eight patients with r/r disease, three achieved a CR, two of them had MRD negativity and two had ongoing responses for up to almost 4 months. In this work the most common grade 3 adverse event (AE) was febrile neutropenia (39%), and there were two 4 grade AEs, both infectious [37].
In the ASH 2021 annual meeting, a phase II study that examined the addition of venetoclax to mini-CVD reported the results of 23 patients with Ph-neg ALL, either > 60 years with a previously untreated disease, or > 18 years with r/r disease. In this study, venetoclax was given at a 400 mg dose on days 1–14 of cycle one, and on days 1–7 of the proceeding cycles. In addition, CD20+ B-ALL patients received rituximab, and nelarabine + PEG-asparaginase was incorporated into consolidation and maintenance for T-ALL patients. Patients were intended to proceed to allo-SCT or to receive VCR, prednisolone and venetoclax maintenance. Among the four previously untreated patients, three attained CR with MRD negativity and one achieved PR; all responses were achieved after one cycle. At a median follow up of 1 year, all CR patients remained in remission. Among the relapsed cohort, 11 (65%) responded (CR/CRi) and 3 achieved MRD negativity, and all responses were within the first two cycles. The reported median progression free survival (PFS) in the r/r cohort was 6 months, and overall survival (OS) was 7 months [38]. The conclusion of these prospective studies was that mini-CVD with venetoclax is a safe and effective regimen for Ph-neg ALL patients.
With the scarcity of alternatives, especially in the r/r setup, several reports on ETP or T-ALL patients salvaged with chemotherapy in combination with venetoclax were published. A case series of five adult patients with r/r ETP-ALL treated with venetoclax in combination with chemotherapy reported an 80% (4/5) CR rate. The patients were treated for 2–4 cycles with venetoclax and hyper-CVAD (2 pts), mini-CVD (2 pts) or nelarabine (1 pt). MRD negativity was achieved in two cases, where both patients were referred to allo-SCT. One of the CR-MRD+ patients was referred to allo-SCT and was in remission at a 3 mo post-transplant follow up. The other CR-MRD+ patient was not eligible for SCT and received venetoclax with vincristine and prednisone maintenance and remained in remission for 5 months until relapse. None of the patients in this series developed tumor lysis syndrome under venetoclax treatment, and there were no reported toxicities other than transient cytopenia [39]. In another report from China, a 25 yo male with relapsed ETP-ALL was successfully treated with venetoclax 400 mg and HAG chemotherapy (aclacinomycin, cytarabine and G-CSF). CR was attained after one cycle and the patient went to allo-SCT, and on a follow up 5 months post-transplant was in remission [40].
Two retrospective analyses from MD Anderson described their experience with venetoclax combinations in ALL. In a report of 13 adult patients (median age 46) treated with venetoclax and chemotherapy for r/r T-ALL, 60% of evaluable patients achieved CR. The concurrent chemotherapy regimens included hyper-CVAD, asparaginase with vincristine or nelarabine, decitabine or FLAG-Ida. Venetoclax was given in a 100–400 mg dose, for a median of 21 days per cycle. The main AE observed was prolonged cytopenia, there were no deaths within 30 days of venetoclax administration and all subsequent deaths were due to disease progression. Among the eight responders, the median PFS was 4 months, and the median OS of the entire cohort was 7.7 months [41]. In an analysis of 18 pediatric and young adults (<22) with a median age of 20 and who received venetoclax in combination with chemotherapy, 11 (61%) responded (CR/Cri) and all achieved MRD negativity. Most of the patients received hyper-CVAD or CVD as concurrent chemotherapy, and 400 mg was the venetoclax dosage for the majority of patients. Among the 13 T-ALL (including ETP-ALL) patients, the response rate was 77%. The most common AE was thrombocytopenia, with 22% requiring a dose adjustment. Neutropenia and febrile neutropenia were 50% and 22%, respectively. With a median follow up of 12.11 mo, the OS is 9.14 mo, and PFS is 7.3 mo [42].
Few other chemotherapeutic regimens were examined in combination with venetoclax in prospective studies. In a phase 1 dose escalation study of venetoclax with L-vincristine, 18 adult patients (median age 42) with r/r B or T-ALL were included. Patients received 2 weeks of a lead-in phase of venetoclax in increasing dosage until reaching 400–800 mg daily. L-Vincristine was added to the treatment when the venetoclax full dose was reached. Grade 3 or more AEs were reported in 89% of the patients, with cytopenia being the most common. One patient experienced tumor lysis syndrome. Two patients had rapidly progressing disease under the treatment. Two patients had to stop the treatment due to toxicity. Four patients (22%) attained CR under the treatment, and two of them had MRD negativity [43].
The largest study published to date is a phase 1, multicenter study that examined the combination of venetoclax and navitoclax with chemotherapy (VCR, dexamethasone +/- Peg-asparaginase). In this study, 47 patients, 12 of whom were under 18 years of age, with r/r ALL were included. Patients received venetoclax 400 mg daily with escalating doses of navitoclax (25–100 mg daily) in combination with chemotherapy. Thirty-seven patients (78%) experienced serious AEs; the most common grade 3/4 AEs were febrile neutropenia (46.8%), thrombocytopenia (38.3%) and neutropenia (19%). Venetoclax and navitoclax were discontinued due to AEs in 11 patients, where 6 were considered related to venetoclax and 5 to navitoclax. During the study, 30 patients (63%) died, 26 of them from disease progression. A response (CR, CRi, CRp) was achieved by 28 patients (60%), including 9 children. The overall response rate (ORR) among those 18 yo and younger was 75%. MRD negativity was achieved by 16 patients (34%), where 6 of them were 18 and younger. A post hoc analysis of patients’ subgroups revealed no differences between B- or T-cell disease. The median duration of response was 4.2 mo, and OS was 7.8 mo. Correlative BH3 profiling demonstrated frequent BCL-2 and BCL-XL dependency in ALL cells, and the capacity for dependency switching during treatment as a potential mechanism of resistance to BCL-2 inhibition [44].
The combination of chemotherapy and targeted therapies with venetoclax was also suggested in a recent report. A 26 yo r/r pro-BALL patient was treated with venetoclax (100 mg, days 3–7) and the anti-CD38 antibody daratumumab (days 2 and 10) with cyclophosphamide (150 mg/m2, days 1–3) and clofarabine (20 mg/m2, days 1–5). After prolonged agranulocytosis of 43 days, hematopoietic reconstitution with CR was attained. The patient remained MRD-negative for 5 months as he was waiting for allo-SCT. In a follow up 12 months after SCT, he was still in remission under venetoclax maintenance [45].
Taken together, the administration of venetoclax in combination with low-dose chemotherapy regimens for Ph-neg B or T-ALL seems to be feasible and effective. It is especially important in the setup of r/r ETP-ALL with the scarceness of available therapeutic options.

4.2. Chemotherapy-Free Regimens

Elderly patients with Ph-neg ALL have a dismal prognosis, with high rates of early death. A first report of chemotherapy-free regimens for the treatment of Ph-neg ALL included three newly diagnosed elderly patients that were ineligible for intensive chemotherapy. The patients were treated with venetoclax 100 mg on day 1, and 200 mg days 2–28, with prednisone 1 mg/kg days 1–21, and 0.5 mg/kg days 22–28. Two patients achieved CR after the first cycle, one of which had MRD negativity. No AEs were noted, and the clinical status of both patients was significantly improved under treatment. Nevertheless, both patients relapsed after 2 months, and died of active disease. The third patient was refractory to the venetoclax + prednisone regimen [46]. With the limitation of only three patients, this report warrants further investigations of the venetoclax + prednisone regimen for selective populations.
Venetoclax with azacitidine, which became the standard-of-care regimen for AML patients who are ineligible for intensive chemotherapy, has significantly changed the survival rates of the disease. In a thorough review of the literature, we have found four case reports of r/r T-ALL patients treated with the combination of venetoclax and decitabine, and one case series of five r/r T-ALL patients treated with venetoclax with azacitidine.
The first report from UCSF was a 20 yo male, initially diagnosed with AML, but in retrospect, had T-ALL. He achieved CR after induction chemotherapy and was consolidated with SCT, but relapsed 13 months after with a disease that was found to be T-ALL. He achieved CR with MRD negativity following treatment with venetoclax (800 mg, days 1–28) and decitabine (20 mg/m2, days 1–5), and continued the treatment for five cycles until his second allo-SCT [47]. The second report was of a 36 yo female with r/r ETP-ALL; she was given venetoclax 600 mg daily with similar doses of decitabine. Although she attained CR with MRD negativity after one cycle, the second cycle was interrupted due to cytopenia and systemic infections, which she eventually died of [48]. Two case reports of young women treated with venetoclax and decitabine for r/r ETP-ALL described CR after up to two cycles, with no severe AEs, and sustained remission 3 months after allo-SCT [49,50]. Venetoclax with azacitidine was given to five primary refractory T-ALL patients in China. Four patients achieved CR and remained in remission under treatment for 1–7 months at the time of report. The fifth patient achieved Cri, was referred to allo-SCT and died of a-GVHD. Cytopenia occurred in all five patients under the Ven-Aza treatment, with a median of 17 days to neutrophil recovery after initiation of therapy. No other grade 3/4 AEs were reported [51]. Preliminary data suggest that the synergy between venetoclax and HMA therapy may also be beneficial in ALL and can present an effective alternative for chemo-resistant patients, especially in elderly or frail patients.
Ph(+) ALL outcomes have greatly improved in the TKIs era, and the incorporation of blinatumomab in the treatments made chemotherapy-free regimens available [52]. Nevertheless, r/r Ph(+) ALL still carries a poor prognosis and improved approaches are needed. The chemotherapy-free combination of venetoclax with ponatinib and dexamethasone (VPD) for r/r Ph+ ALL was examined in a phase 1 study. Nine adult patients (median age 37) with at least one prior Tki treatment were included in the analysis. Two doses of venetoclax, 400 mg (three patients) and 800 mg (three patients), were investigated. The combination was given on a 28-day cycle protocol. For CD20+ patients, rituximab was added. Most AEs were grade 1–2 and related to cytopenia. No dose-limiting toxicities were observed, and the maximum-tolerated dose of venetoclax was not reached. Five patients (56%) responded (CR/Cri), and all were in the 800 mg dose level. Four patients (44%) achieved complete molecular response (CMR). With a median follow up of 13.2 mo, two patients died from progressive disease, none have relapsed, and the median OS was not reached. All responding patients remained in response without allo-SCT [53]. In a retrospective analysis of 19 r/r Ph(+) ALL patients, the outcomes of a treatment with VPD were reported. After one cycle of treatment, 17 patients (89.5%) attained CR/Cri, with 14 patients having MRD negativity, and 8 patients had CMR. Grade 3 and more AEs were mostly due to myelosuppression with 73% neutropenia and 52% thrombocytopenia. Among 11 patients who continued with VPD, 7 subsequently relapsed, whereas among the patients who went to allo-SCT, only 1 of 6 patients relapsed. This suggests that bridging to allo-SCT in remission is warranted [54].
Finally, numerous ongoing trials are incorporating venetoclax and other BCL-2 family inhibitors in various regimens, including chemotherapy, immunotherapy (blinatumomab), hypomethylating agents and others (Table 2).
Table
Table 1. Significant clinical studies with venetoclax-based combinations.
Table 1. Significant clinical studies with venetoclax-based combinations.
First Author, YearCohortDesign, RegimenORROSGrade III/IV AE
Jain 2019 [37]n = 18 Ph-neg ALL > 18 yo r/r or > 60 yo NDPhase 1, Ven 400/600 mg with mini-hyper-CVDr/r: 37.5% ND: 100% 39% FN, 17% hyperglycemia, 11% hypocalcemia, 1 pneumonia, 1 sepsis
Carpentier 2019 [41]n = 13 r/r T-ALL adultsRetrospective, Ven 100–400 mg with chemotherapy/HMA60%7.7 moProlonged cytopenia (percentage not reported)
Venugopal 2021 [38]n = 23 Ph-neg ALL > 18 yo r/r or > 60 yo NDPhase 2, Ven 400 mg with mini-hyper-CVD65%7.1 moCytopenia (percentage not reported), 1 sepsis
Gibson 2021 [42]n = 18 ALL age < 22Retrospective, Ven 400 mg with chemotherapy61% 89% thrombocytopenia, 53% neutropenia, 22% hyperbilirubinemia, 28% sepsis, 28% FN, 1 pneumonia, 1 coagulopathy, 1 mucosal infection
Pullarkat 2021 [44]n = 47 r/r ALL, all agesPhase 1, Ven + navitoclax with chemotherapy59.6%7.8 mo46.8% FN, 38.3% neutropenia, 25.5% thrombocytopenia
Palmisiano 2021 [43]n = 18 r/r Ph-neg ALL adultsPhase 1, Ven + L-vincristine44% 67% neutropenia, 56% leukopenia, 50% anemia, 1 TLS
Short 2021 [53]n = 9 r/r Ph(+) ALL adultsPhase 1, Ven + ponatinib + dex56% 44% cytopenia
Wang 2022 [54]n = 19 r/r Ph(+) ALL adultsRetrospective, Ven + ponatinib + dex89.5%400 days73% neutropenia, 36% anemia, 52% thrombocytopenia
n—number of patients; Ph-neg—Philadelphia chromosome-negative; ALL—acute lymphoblastic leukemia; yo—years old; r/r—relapsed/refractory; ND—newly diagnosed; Ven—venetoclax; CVD—cyclophosphamide, vincristine and dexamethasone; HMA—hypomethylating agent; dex—dexamethasone; ORR—overall response rate; OS—overall survival; mo—months; AE—adverse event; FN—febrile neutropenia.
Table
Table 2. Ongoing registered clinical trials with venetoclax-based treatments in ALL.
Table 2. Ongoing registered clinical trials with venetoclax-based treatments in ALL.
PopulationInterventionStudy TypePrimary OutcomePrimary Investigator
NCT05433532Adults with newly diagnosed Ph+ ALL, AML and CML-AP/BP patientsVenetoclax, azacytidine, and flumatinib induction and consolidationPhase 2, open-labelComplete molecular remission at end of cycle 2Xiaowen Tang, The First Affiliated Hospital of Soochow University
NCT03826992Children and young adults with R/R AML, MPAL, AUL, KMT2A-rearranged ALL, T-cell ALL or ETP-ALLVenetoclax with CPX-351Phase 1, open-label,Feasibility, toxicityJohn Perentesis, Children’s Hospital Medical Center, Cincinnati
NCT04029688Children and young adults with newly diagnosed neuroblastoma, AML or ALLIdasanutlin in combination with chemotherapy or venetoclaxPhase ½, open-labelFeasibility, toxicityHoffmann-La Roche
NCT04000698Children with CD38+, CD184+ and Bcl2+, R/R AML or ALLHaploidentical HSCT with conditioning chemotherapy including venetoclaxPhase 1/2, open-labelEngraftment at day + 30 after HSCT, ORR, PRR, toxicity, transplant-related mortalityMichael Maschan, Federal Research Institute of Pediatric Hematology, Oncology and Immunology, Moscow
NCT05292664Children and young adults with R/R ALL and other hematological malignanciesVenetoclax combined with chemotherapyPhase 1, open-labelTolerated dose, toxicityAndrew E Place, Dana-Farber Cancer Institute
NCT05386576Adults with newly diagnosed ALLVenetoclax combined with asparaginase-containing pediatric-inspired chemotherapyPhase 1, open-labelDose-limiting toxicityJae Park, Memorial Sloan Kettering Cancer Center
NCT05192889Children with R/R ALLPhase 1: chemotherapy with venetoclax and navitoclax Phase 2: venetoclax with either blinatumomab (for CD19+) or high-dose cytarabine and navitoclax (for CD19-)Phase 1/2, open-labelMRD negative CR/Cri rate following induction, recommended phase 2 doseSeth E. Karol, St. Jude Children’s Research Hospital
NCT05005299Adults with hematological malignancies who are planned for ASCTVenetoclax therapy prior to non-myeloablative conditioning with fludarabine and cyclophosphamidePhase 1, open-labelToxicityDavid Ritchie, Melbourne Health
NCT05149378Adults and young adults with R/R T-ALLVenetoclax with azacitidinePhase 2, open-labelORR, CR rateSheng-Li Xue, The First Affiliated Hospital of Soochow University
NCT03181126(completed)Adults and children with R/R ALLVenetoclax with navitoclax and chemotherapyPhase 1, open-labelPharmacokinetics, toxicityAbbvie
NCT05268003Adults and children with R/R T-ALLPonatinib With mini-hyper-CVD and venetoclaxPhase 2, open-labelCR, Cri rateJain Nitin, MD Anderson Cancer Center
NCT05157971Adults (18–54) with newly diagnosed B-ALLVenetoclax with the C10403 regimen for induction and consolidationPhase 1, open-labelSafety, maximal tolerated doseIbrahim T Aldoss, City of Hope Medical Center
NCT00501826Children and adults with newly diagnosed T-ALLHyper-CVAD in combination with nelarabine, venetoclax and PEG-asparaginasePhase 2, open-labelCRR, duration of remission, PFS, OSFarhad Ravandi-Kashani, MD Anderson Cancer Center
NCT03319901Newly diagnosed ALL >60 yo or R/R ALL >18 yoVenetoclax with standard chemotherapyPhase 1, open-labelTolerated doseDaniel DeAngelo, Dana-Farber Cancer Institute
NCT05182385Adults with R/R Ph- B-cell precursor ALLVenetoclax with blinatumomabPhase 1/2, open-labelTolerated dose, rate of complete molecular remission after 1 cycleNicola Goekbuget, GMALL study-group
NCT03576547Adults with Ph+ or BCR-ABL+ R/R ALL or CMLVenetoclax, ponatinib and dexamethasonePhase 1/2, open-labelTolerated dose, ORRFarhad Ravandi-Kashani, MD Anderson Cancer Center
NCT04752163Adults with R/R AML or ALLDS-1594b with or without zacytidine, venetoclax, or mini-HCVDPhase 1/2, open-labelTolerated dose, toxicity, CR/Cri rate,Naval G Daver, MD Anderson Cancer Center
NCT05016947Adults with R/R CD22+ B-ALLVenetoclax with inotuzumabPhase 1, open-labelTolerated dose, toxicityMarlise R Luskin, Dana-Farber Cancer Institute
NCT03504644Adults with R/R ALLVenetoclax with liposomal vincristinePhase 1b/2, open -labelTolerated dose, toxicity, CR/Cri rateNeil Palmisiano, ECOG-ACRIN Cancer Research Group
NCT04872790Adults with newly diagnosed or relapsed Ph+ ALLVenetoclax with dasatinib, prednisone and rituximabPhase 1b, open-labelTolerated dose, toxicityJessica T Leonard, OHSU Knight Cancer Institute
NCT05376111Adults and young adults with newly diagnosed T-ALLVenetoclax with azacitidinePhase 2, open-labelORRSheng-Li Xue, The First Affiliated Hospital of Soochow University
NCT03808610Adults with R/R ALLVenetoclax with low-intensity chemotherapy (cyclophosphamide, cytarabine, methotrexate, PEG-asparaginase, vincristine, rituximab)Phase 1/2, open-labelTolerated dose, toxicityElias Jabbour, MD Anderson Cancer Center
NCT05054465Adults with high-risk T-ALL post-remissionVenetoclax and navitoclax pre- and post-ASCT maintenancePhase 1b/2, open-labelEFSOfir Wolach, Israeli Medical Association
Ph—Philadelphia chromosome, ALL—acute lymphoblastic leukemia, AML—acute myeloid leukemia, CML—chronic myeloid leukemia, AP—accelerated phase, BP—blast phase, MPAL—mixed-phenotype acute leukemia, AUL—acute undifferentiated leukemia, ETP—early thymocyte precursor, HSCT—hematopoietic stem cell transplantation, ORR—overall response rate, PRR—partial response rate, MRD—minimal residual disease, CR—complete remission, Cri—complete remission with incomplete count recovery, PFS—progression free survival, OS—overall survival, R/R—relapsed/refractory, EFS—event-free survival.

5. Discussion

Preclinical studies demonstrate that BCL-2 family proteins play a significant role in ALL blasts, including at early leukemia initiation stages. As data accumulate, two inherent questions arise: can we identify which ALL sub-types would respond to BCL-2 inhibition, and what drug combinations are optimal? Certain molecular subtypes such as MLL-rearranged, TCF3-HLF and hypodiploid are correlated with BCL-2 overexpression, with preclinical evidence supporting the use of venetoclax in these subtypes. Ongoing clinical trials were designed to specifically target these subtypes. Similarly, early T-cell ALL is more dependent on BCL-2 and shows sensitivity to venetoclax, including in small clinical series [27,36,39,40]. Ph+ ALL and Ph-like tend to be more dependent on other BCL-2 proteins such as MCL-1 and BCL-XL, with an expected lower response to venetoclax as a single agent [19]. MCL-1 and BCL-XL can sequester the pro-apoptotic effectors that are released upon the binding of venetoclax to BCL-2, rendering it ineffective. Lessons from CLL and AML offer additional novel insights on mechanisms of resistance. p53 mutations in AML were shown to reduce BCL-2 levels and increase MCL-1 levels, promoting venetoclax resistance [55,56]. Additionally, BCL-2 levels’ independent mechanisms have also been described, such as the change in the upregulation of fatty acid oxidation [57]. Venetoclax in itself was shown to upregulate MCL-1 levels [25]. Our growing understanding of BCL-2 proteins’ interplay and experience in AML has prompted clinical trials adding venetoclax to commonly used chemotherapy protocols.
Preliminary studies of venetoclax added to mini-hyper-CVD showed encouraging results, including in the r/r setting [36]. Others have attempted to combine different agents with the aim to overcome the resistance mechanisms described above. Navitoclax, which inhibits both BCL-2 and BCL-XL, was used in combination with venetoclax in r/r ALL with a remarkable complete remission rate of 60% [44]. Adding venetoclax to hypomethylating agents was tried in r/r T-ALL, including p53 mutation [48,50]. The MCL-1 specific inhibitor also showed a synergistic effect in vitro with venetoclax and holds promise as a future chemotherapy-free combination. Similarly, TKI treatments in Ph+ ALL were shown to reduce MCL-1 levels, sensitizing the cell to venetoclax. Clinical data in Ph+ ALL patients, including in those with T315I mutations, is limited but encouraging [54]. As described in Table 2, there are numerous clinical trials studying the use of venetoclax in specific subtypes and combinations. The results of these trials will help us to better tailor BCL-2 inhibition to the specific patient.

6. Conclusions

Clinical experience of venetoclax in ALL is still emerging, but the current available data support the hypothesis of BH3-mimetic activity in ALL. Venetoclax-containing regimens are relatively safe and can achieve deep responses, although these are frequently transient, and therefore, should be utilized as a bridge to all-SCT in eligible patients. Our growing understanding of the interplay of BCL-2 family proteins in ALL will allow us to better tailor BCL-2 inhibitors and combinations to a specific subtype. Ongoing clinical trials will shape how we incorporate these agents in the coming years.

Author Contributions

S.A.: writing—review and editing, A.H.: data curation, N.G.E.-Z.: writing—review and editing, A.S.: writing—review and editing, V.V.: review and editing, B.N.: conceptualization, writing—review and editing. All authors have read and agreed to the published version of the manuscript.

Funding

Received honorary for lectures from Abbvie, Roche and Janssen.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

B.N—Abbvie advisory board member and honoraria. Other authors have no conflict of interest to declare.

References

  1. Youle, R.J.; Strasser, A. The BCL-2 Protein Family: Opposing Activities That Mediate Cell Death. Nat. Rev. Mol. Cell Biol. 2008, 9, 47–59. [Google Scholar] [CrossRef]
  2. Moia, R.; Diop, F.; Favini, C.; Kodipad, A.A.; Gaidano, G. Potential of BCL2 as a Target for Chronic Lymphocytic Leukemia Treatment. Expert Rev. Hematol. 2018, 11, 391–402. [Google Scholar] [CrossRef] [PubMed]
  3. Roberts, A.W.; Wei, A.H.; Huang, D.C.S. BCL2 and MCL1 Inhibitors for Hematologic Malignancies. Blood 2021, 138, 1120–1136. [Google Scholar] [CrossRef] [PubMed]
  4. High, L.M.; Szymanska, B.; Wilczynska-Kalak, U.; Barber, N.; O’Brien, R.; Khaw, S.L.; Vikstrom, I.B.; Roberts, A.W.; Lock, R.B. The Bcl-2 Homology Domain 3 Mimetic ABT-737 Targets the Apoptotic Machinery in Acute Lymphoblastic Leukemia Resulting in Synergistic in Vitro and in Vivo Interactions with Established Drugs. Mol. Pharmacol. 2010, 77, 483–494. [Google Scholar] [CrossRef] [PubMed]
  5. DiNardo, C.D.; Lachowiez, C.A.; Takahashi, K.; Loghavi, S.; Xiao, L.; Kadia, T.; Daver, N.; Adeoti, M.; Short, N.J.; Sasaki, K.; et al. Venetoclax Combined With FLAG-IDA Induction and Consolidation in Newly Diagnosed and Relapsed or Refractory Acute Myeloid Leukemia. J. Clin. Oncol. 2021, 39, 2768–2778. [Google Scholar] [CrossRef]
  6. DiNardo, C.D.; Jonas, B.A.; Pullarkat, V.; Thirman, M.J.; Garcia, J.S.; Wei, A.H.; Konopleva, M.; Döhner, H.; Letai, A.; Fenaux, P.; et al. Azacitidine and Venetoclax in Previously Untreated Acute Myeloid Leukemia. N. Engl. J. Med. 2020, 383, 617–629. [Google Scholar] [CrossRef]
  7. Fischer, K.; Al-Sawaf, O.; Bahlo, J.; Fink, A.-M.; Tandon, M.; Dixon, M.; Robrecht, S.; Warburton, S.; Humphrey, K.; Samoylova, O.; et al. Venetoclax and Obinutuzumab in Patients with CLL and Coexisting Conditions. N. Engl. J. Med. 2019, 380, 2225–2236. [Google Scholar] [CrossRef]
  8. Seymour, J.F.; Kipps, T.J.; Eichhorst, B.; Hillmen, P.; D’Rozario, J.; Assouline, S.; Owen, C.; Gerecitano, J.; Robak, T.; De la Serna, J.; et al. Venetoclax–Rituximab in Relapsed or Refractory Chronic Lymphocytic Leukemia. N. Engl. J. Med. 2018, 378, 1107–1120. [Google Scholar] [CrossRef]
  9. Suryani, S.; Carol, H.; Chonghaile, T.N.; Frismantas, V.; Sarmah, C.; High, L.; Bornhauser, B.; Cowley, M.J.; Szymanska, B.; Evans, K.; et al. Cell and Molecular Determinants of in Vivo Efficacy of the BH3 Mimetic ABT-263 against Pediatric Acute Lymphoblastic Leukemia Xenografts. Clin. Cancer Res. Off. J. Am. Assoc. Cancer Res. 2014, 20, 4520–4531. [Google Scholar] [CrossRef]
  10. Frismantas, V.; Dobay, M.P.; Rinaldi, A.; Tchinda, J.; Dunn, S.H.; Kunz, J.; Richter-Pechanska, P.; Marovca, B.; Pail, O.; Jenni, S.; et al. Ex Vivo Drug Response Profiling Detects Recurrent Sensitivity Patterns in Drug-Resistant Acute Lymphoblastic Leukemia. Blood 2017, 129, e26–e37. [Google Scholar] [CrossRef] [Green Version]
  11. Peirs, S.; Matthijssens, F.; Goossens, S.; Van de Walle, I.; Ruggero, K.; de Bock, C.E.; Degryse, S.; Canté-Barrett, K.; Briot, D.; Clappier, E.; et al. ABT-199 Mediated Inhibition of BCL-2 as a Novel Therapeutic Strategy in T-Cell Acute Lymphoblastic Leukemia. Blood 2014, 124, 3738–3747. [Google Scholar] [CrossRef] [PubMed]
  12. Beldjord, K.; Chevret, S.; Asnafi, V.; Huguet, F.; Boulland, M.-L.; Leguay, T.; Thomas, X.; Cayuela, J.-M.; Grardel, N.; Chalandon, Y.; et al. Oncogenetics and Minimal Residual Disease Are Independent Outcome Predictors in Adult Patients with Acute Lymphoblastic Leukemia. Blood 2014, 123, 3739–3749. [Google Scholar] [CrossRef] [PubMed]
  13. Benito, J.M.; Godfrey, L.; Kojima, K.; Hogdal, L.; Wunderlich, M.; Geng, H.; Marzo, I.; Harutyunyan, K.G.; Golfman, L.; North, P.; et al. MLL-Rearranged Acute Lymphoblastic Leukemias Activate BCL-2 through H3K79 Methylation and Are Sensitive to the BCL-2-Specific Antagonist ABT-199. Cell Rep. 2015, 13, 2715–2727. [Google Scholar] [CrossRef] [PubMed]
  14. Alford, S.E.; Kothari, A.; Loeff, F.C.; Eichhorn, J.M.; Sakurikar, N.; Goselink, H.M.; Saylors, R.L.; Jedema, I.; Falkenburg, J.H.F.; Chambers, T.C. BH3 Inhibitor Sensitivity and Bcl-2 Dependence in Primary Acute Lymphoblastic Leukemia Cells. Cancer Res. 2015, 75, 1366–1375. [Google Scholar] [CrossRef] [PubMed]
  15. Robinson, B.W.; Behling, K.C.; Gupta, M.; Zhang, A.Y.; Moore, J.S.; Bantly, A.D.; Willman, C.L.; Carroll, A.J.; Adamson, P.C.; Barrett, J.S.; et al. Abundant Anti-Apoptotic BCL-2 Is a Molecular Target in Leukaemias with t(4;11) Translocation. Br. J. Haematol. 2008, 141, 827–839. [Google Scholar] [CrossRef]
  16. Dawson, M.A.; Prinjha, R.K.; Dittmann, A.; Giotopoulos, G.; Bantscheff, M.; Chan, W.-I.; Robson, S.C.; Chung, C.; Hopf, C.; Savitski, M.M.; et al. Inhibition of BET Recruitment to Chromatin as an Effective Treatment for MLL-Fusion Leukaemia. Nature 2011, 478, 529–533. [Google Scholar] [CrossRef]
  17. de Boer, J.; Yeung, J.; Ellu, J.; Ramanujachar, R.; Bornhauser, B.; Solarska, O.; Hubank, M.; Williams, O.; Brady, H.J.M. The E2A-HLF Oncogenic Fusion Protein Acts through Lmo2 and Bcl-2 to Immortalize Hematopoietic Progenitors. Leukemia 2011, 25, 321–330. [Google Scholar] [CrossRef]
  18. Fischer, U.; Forster, M.; Rinaldi, A.; Risch, T.; Sungalee, S.; Warnatz, H.-J.; Bornhauser, B.; Gombert, M.; Kratsch, C.; Stütz, A.M.; et al. Genomics and Drug Profiling of Fatal TCF3-HLF-Positive Acute Lymphoblastic Leukemia Identifies Recurrent Mutation Patterns and Therapeutic Options. Nat. Genet. 2015, 47, 1020–1029. [Google Scholar] [CrossRef]
  19. Diaz-Flores, E.; Comeaux, E.Q.; Kim, K.L.; Melnik, E.; Beckman, K.; Davis, K.L.; Wu, K.; Akutagawa, J.; Bridges, O.; Marino, R.; et al. Bcl-2 Is a Therapeutic Target for Hypodiploid B-Lineage Acute Lymphoblastic Leukemia. Cancer Res. 2019, 79, 2339–2351. [Google Scholar] [CrossRef]
  20. Hohtari, H.; Kankainen, M.; Adnan-Awad, S.; Yadav, B.; Potdar, S.; Ianevski, A.; Dufva, O.; Heckman, C.; Sexl, V.; Kytölä, S.; et al. Targeting Apoptosis Pathways With BCL2 and MDM2 Inhibitors in Adult B-Cell Acute Lymphoblastic Leukemia. HemaSphere 2022, 6, e701. [Google Scholar] [CrossRef]
  21. Koss, B.; Morrison, J.; Perciavalle, R.M.; Singh, H.; Rehg, J.E.; Williams, R.T.; Opferman, J.T. Requirement for Antiapoptotic MCL-1 in the Survival of BCR-ABL B-Lineage Acute Lymphoblastic Leukemia. Blood 2013, 122, 1587–1598. [Google Scholar] [CrossRef] [PubMed]
  22. Del Gaizo Moore, V.; Schlis, K.D.; Sallan, S.E.; Armstrong, S.A.; Letai, A. BCL-2 Dependence and ABT-737 Sensitivity in Acute Lymphoblastic Leukemia. Blood 2008, 111, 2300–2309. [Google Scholar] [CrossRef] [PubMed]
  23. Mérino, D.; Khaw, S.L.; Glaser, S.P.; Anderson, D.J.; Belmont, L.D.; Wong, C.; Yue, P.; Robati, M.; Phipson, B.; Fairlie, W.D.; et al. Bcl-2, Bcl-x(L), and Bcl-w Are Not Equivalent Targets of ABT-737 and Navitoclax (ABT-263) in Lymphoid and Leukemic Cells. Blood 2012, 119, 5807–5816. [Google Scholar] [CrossRef] [PubMed]
  24. Seyfried, F.; Demir, S.; Hörl, R.L.; Stirnweiß, F.U.; Ryan, J.; Scheffold, A.; Villalobos-Ortiz, M.; Boldrin, E.; Zinngrebe, J.; Enzenmüller, S.; et al. Prediction of Venetoclax Activity in Precursor B-ALL by Functional Assessment of Apoptosis Signaling. Cell Death Dis. 2019, 10, 571. [Google Scholar] [CrossRef]
  25. Choudhary, G.S.; Al-harbi, S.; Mazumder, S.; Hill, B.T.; Smith, M.R.; Bodo, J.; Hsi, E.D.; Almasan, A. MCL-1 and BCL-XL-Dependent Resistance to the BCL-2 Inhibitor ABT-199 Can Be Overcome by Preventing PI3K/AKT/MTOR Activation in Lymphoid Malignancies. Cell Death Dis. 2015, 6, e1593. [Google Scholar] [CrossRef]
  26. Khaw, S.L.; Suryani, S.; Evans, K.; Richmond, J.; Robbins, A.; Kurmasheva, R.T.; Billups, C.A.; Erickson, S.W.; Guo, Y.; Houghton, P.J.; et al. Venetoclax Responses of Pediatric ALL Xenografts Reveal Sensitivity of MLL-Rearranged Leukemia. Blood 2016, 128, 1382–1395. [Google Scholar] [CrossRef]
  27. Chonghaile, T.N.; Roderick, J.E.; Glenfield, C.; Ryan, J.; Sallan, S.E.; Silverman, L.B.; Loh, M.L.; Hunger, S.P.; Wood, B.; DeAngelo, D.J.; et al. Maturation Stage of T-Cell Acute Lymphoblastic Leukemia Determines BCL-2 versus BCL-XL Dependence and Sensitivity to ABT-199. Cancer Discov. 2014, 4, 1074–1087. [Google Scholar] [CrossRef]
  28. Li, Z.; He, S.; Look, A.T. The MCL1-Specific Inhibitor S63845 Acts Synergistically with Venetoclax/ABT-199 to Induce Apoptosis in T-Cell Acute Lymphoblastic Leukemia Cells. Leukemia 2019, 33, 262–266. [Google Scholar] [CrossRef]
  29. Lázaro-Navarro, J.; Pimentel-Gutiérrez, H.J.; Gauert, A.; Hagemann, A.I.H.; Eisenschmid, J.; Gökbuget, N.; Vick, B.; Jeremias, I.; Seyfried, F.; Meyer, L.H.; et al. Inhibiting Casein Kinase 2 Sensitizes Acute Lymphoblastic Leukemia Cells to Venetoclax via MCL1 Degradation. Blood Adv. 2021, 5, 5501–5506. [Google Scholar] [CrossRef]
  30. Moujalled, D.M.; Hanna, D.T.; Hediyeh-Zadeh, S.; Pomilio, G.; Brown, L.; Litalien, V.; Bartolo, R.; Fleming, S.; Chanrion, M.; Banquet, S.; et al. Cotargeting BCL-2 and MCL-1 in High-Risk B-ALL. Blood Adv. 2020, 4, 2762–2767. [Google Scholar] [CrossRef]
  31. Massimino, M.; Tirrò, E.; Stella, S.; Pennisi, M.S.; Vitale, S.R.; Puma, A.; Romano, C.; DI Gregorio, S.; Romeo, M.A.; DI Raimondo, F.; et al. Targeting BCL-2 as a Therapeutic Strategy for Primary P210BCR-ABL1-Positive B-ALL Cells. Vivo Athens Greece 2020, 34, 511–516. [Google Scholar] [CrossRef] [PubMed]
  32. Goff, D.J.; Court Recart, A.; Sadarangani, A.; Chun, H.-J.; Barrett, C.L.; Krajewska, M.; Leu, H.; Low-Marchelli, J.; Ma, W.; Shih, A.Y.; et al. A Pan-BCL2 Inhibitor Renders Bone-Marrow-Resident Human Leukemia Stem Cells Sensitive to Tyrosine Kinase Inhibition. Cell Stem Cell 2013, 12, 316–328. [Google Scholar] [CrossRef] [PubMed]
  33. Leonard, J.T.; Rowley, J.S.J.; Eide, C.A.; Traer, E.; Hayes-Lattin, B.; Loriaux, M.; Spurgeon, S.E.; Druker, B.J.; Tyner, J.W.; Chang, B.H. Targeting BCL-2 and ABL/LYN in Philadelphia Chromosome–Positive Acute Lymphoblastic Leukemia. Sci. Transl. Med. 2016, 8, 354ra114. [Google Scholar] [CrossRef] [PubMed]
  34. Peirs, S.; Frismantas, V.; Matthijssens, F.; Van Loocke, W.; Pieters, T.; Vandamme, N.; Lintermans, B.; Dobay, M.P.; Berx, G.; Poppe, B.; et al. Targeting BET Proteins Improves the Therapeutic Efficacy of BCL-2 Inhibition in T-Cell Acute Lymphoblastic Leukemia. Leukemia 2017, 31, 2037–2047. [Google Scholar] [CrossRef] [PubMed]
  35. Kang, M.H.; Kang, Y.H.; Szymanska, B.; Wilczynska-Kalak, U.; Sheard, M.A.; Harned, T.M.; Lock, R.B.; Reynolds, C.P. Activity of Vincristine, L-ASP, and Dexamethasone against Acute Lymphoblastic Leukemia Is Enhanced by the BH3-Mimetic ABT-737 in Vitro and in Vivo. Blood 2007, 110, 2057–2066. [Google Scholar] [CrossRef]
  36. Numan, Y.; Alfayez, M.; Maiti, A.; Alvarado, Y.; Jabbour, E.J.; Ferrajoli, A.; Konoplev, S.N.; Kantarjian, H.M.; Bose, P. First Report of Clinical Response to Venetoclax in Early T-Cell Precursor Acute Lymphoblastic Leukemia. JCO Precis. Oncol. 2018, 2, PO.18.00127. [Google Scholar] [CrossRef]
  37. Jain, N.; Stevenson, K.E.; Winer, E.S.; Garcia, J.S.; Stone, R.M.; Jabbour, E.; Ravandi, F.; Stewart, J.M.; Legg, D.R.; Kantarjian, H.M.; et al. A Multicenter Phase I Study Combining Venetoclax with Mini-Hyper-CVD in Older Adults with Untreated and Relapsed/Refractory Acute Lymphoblastic Leukemia. Blood 2019, 134, 3867. [Google Scholar] [CrossRef]
  38. Venugopal, S.; Kantarjian, H.; Short, N.J.; Thompson, P.A.; Pemmaraju, N.; Jain, N.; Wierda, W.G.; Borthakur, G.; Montalban-Bravo, G.; Ravandi, F.; et al. A Phase II Study of Mini-Hyper-CVD Plus Venetoclax in Patients with Philadelphia Chromosome-Negative Acute Lymphoblastic Leukemia. Blood 2021, 138, 1239. [Google Scholar] [CrossRef]
  39. Arora, S.; Vachhani, P.; Bachiashvili, K.; Jamy, O. Venetoclax with Chemotherapy in Relapse/Refractory Early T-Cell Precursor Acute Lymphoblastic Leukemia. Leuk. Lymphoma 2021, 62, 2292–2294. [Google Scholar] [CrossRef]
  40. Zhang, X.; Li, J.; Jin, J.; Yu, W. Relapsed/Refractory Early T-Cell Precursor Acute Lymphoblastic Leukemia Was Salvaged by Venetoclax plus HAG Regimen. Ann. Hematol. 2020, 99, 395–397. [Google Scholar] [CrossRef]
  41. Richard-Carpentier, G.; Jabbour, E.; Short, N.J.; Rausch, C.R.; Savoy, J.M.; Bose, P.; Yilmaz, M.; Jain, N.; Borthakur, G.; Ohanian, M.; et al. Clinical Experience With Venetoclax Combined With Chemotherapy for Relapsed or Refractory T-Cell Acute Lymphoblastic Leukemia. Clin. Lymphoma Myeloma Leuk. 2020, 20, 212–218. [Google Scholar] [CrossRef] [PubMed]
  42. Gibson, A.; Trabal, A.; McCall, D.; Khazal, S.; Toepfer, L.; Bell, D.H.; Roth, M.; Mahadeo, K.M.; Nunez, C.; Short, N.J.; et al. Venetoclax for Children and Adolescents with Acute Lymphoblastic Leukemia and Lymphoblastic Lymphoma. Cancers 2021, 14, 150. [Google Scholar] [CrossRef] [PubMed]
  43. Palmisiano, N.; Lee, J.-W.; Claxton, D.F.; Paietta, E.; Alkhateeb, H.B.; Park, J.H.; Podoltsev, N.; Atallah, E.L.; Schaar, D.G.; Dinner, S.; et al. Maximal Tolerated Dose Determined for Venetoclax in Combination with Liposomal Vincristine in Patients with Relapsed or Refractory Ph-Negative T-Cell or B-Cell Acute Lymphoblastic Leukemia: Results of Phase 1 Portion of ECOG-ACRIN EA9152. Blood 2021, 138, 3407. [Google Scholar] [CrossRef]
  44. Pullarkat, V.A.; Lacayo, N.J.; Jabbour, E.; Rubnitz, J.E.; Bajel, A.; Laetsch, T.W.; Leonard, J.; Colace, S.I.; Khaw, S.L.; Fleming, S.A.; et al. Venetoclax and Navitoclax in Combination with Chemotherapy in Patients with Relapsed or Refractory Acute Lymphoblastic Leukemia and Lymphoblastic Lymphoma. Cancer Discov. 2021, 11, 1440–1453. [Google Scholar] [CrossRef]
  45. Voruz, S.; Blum, S.; de Leval, L.; Schoumans, J.; Solly, F.; Spertini, O. Daratumumab and Venetoclax in Combination with Chemotherapy Provide Sustained Molecular Remission in Relapsed/Refractory CD19, CD20, and CD22 Negative Acute B Lymphoblastic Leukemia with KMT2A-AFF1 Transcript. Biomark. Res. 2021, 9, 92. [Google Scholar] [CrossRef]
  46. Zhang, Y.; Qian, J.-J.; Shen, Y.-J.; Hang, S.-J.; Jin, J.; Zhu, H.-H. The First Report of Complete Remission Following Treatment with Venetoclax plus Prednisone in Elderly Patients with Philadelphia Chromosome-Negative Acute Lymphoblastic Leukemia. Ann. Hematol. 2022, 101, 1141–1144. [Google Scholar] [CrossRef]
  47. Rahmat, L.T.; Nguyen, A.; Abdulhaq, H.; Prakash, S.; Logan, A.C.; Mannis, G.N. Venetoclax in Combination with Decitabine for Relapsed T-Cell Acute Lymphoblastic Leukemia after Allogeneic Hematopoietic Cell Transplant. Case Rep. Hematol. 2018, 2018, 6092646. [Google Scholar] [CrossRef]
  48. Farhadfar, N.; Li, Y.; May, W.S.; Adams, C.B. Venetoclax and Decitabine for Treatment of Relapsed T-Cell Acute Lymphoblastic Leukemia: A Case Report and Review of Literature. Hematol. Oncol. Stem Cell Ther. 2021, 14, 246–251. [Google Scholar] [CrossRef]
  49. Zappone, E.; Cencini, E.; Defina, M.; Sicuranza, A.; Gozzetti, A.; Ciofini, S.; Raspadori, D.; Mecacci, B.; Bocchia, M. Venetoclax in Association with Decitabine as Effective Bridge to Transplant in a Case of Relapsed Early T-Cell Lymphoblastic Leukemia. Clin. Case Rep. 2020, 8, 2000–2002. [Google Scholar] [CrossRef]
  50. Kong, J.; Chen, N.; Li, M.; Zhang, J.; Wu, X.; Zong, L.; Wu, D.; Song, B.; Qiu, H. Venetoclax and Decitabine in Refractory TP53-Mutated Early T-Cell Precursor Acute Lymphoblastic Leukemia. Ann. Hematol. 2022, 101, 697–699. [Google Scholar] [CrossRef]
  51. Wan, C.-L.; Zou, J.-Y.; Qiao, M.; Yin, J.; Shen, X.-D.; Qiu, Q.-C.; Liu, S.-B.; Xue, S.-L. Venetoclax Combined with Azacitidine as an Effective and Safe Salvage Regimen for Relapsed or Refractory T-Cell Acute Lymphoblastic Leukemia: A Case Series. Leuk. Lymphoma 2021, 62, 3300–3303. [Google Scholar] [CrossRef] [PubMed]
  52. Foà, R.; Bassan, R.; Vitale, A.; Elia, L.; Piciocchi, A.; Puzzolo, M.-C.; Canichella, M.; Viero, P.; Ferrara, F.; Lunghi, M.; et al. Dasatinib-Blinatumomab for Ph-Positive Acute Lymphoblastic Leukemia in Adults. N. Engl. J. Med. 2020, 383, 1613–1623. [Google Scholar] [CrossRef] [PubMed]
  53. Short, N.J.; Konopleva, M.; Kadia, T.; Kebriaei, P.; Daver, N.; Huang, X.; Masarova, L.; Cook, R.; Jain, N.; Jabbour, E.; et al. An Effective Chemotherapy-Free Regimen of Ponatinib plus Venetoclax for Relapsed/Refractory Philadelphia Chromosome-Positive Acute Lymphoblastic Leukemia. Am. J. Hematol. 2021, 96, E229–E232. [Google Scholar] [CrossRef] [PubMed]
  54. Wang, H.; Yang, C.; Shi, T.; Zhang, Y.; Qian, J.; Wang, Y.; Hu, Y.; Mao, L.; Ye, X.; Liu, F.; et al. Venetoclax-Ponatinib for T315I/Compound-Mutated Ph+ Acute Lymphoblastic Leukemia. Blood Cancer J. 2022, 12, 20. [Google Scholar] [CrossRef]
  55. Nechiporuk, T.; Kurtz, S.E.; Nikolova, O.; Liu, T.; Jones, C.L.; D’Alessandro, A.; Culp-Hill, R.; d’Almeida, A.; Joshi, S.K.; Rosenberg, M.; et al. The TP53 Apoptotic Network Is a Primary Mediator of Resistance to BCL2 Inhibition in AML Cells. Cancer Discov. 2019, 9, 910–925. [Google Scholar] [CrossRef]
  56. Zhang, Q.; Riley-Gillis, B.; Han, L.; Jia, Y.; Lodi, A.; Zhang, H.; Ganesan, S.; Pan, R.; Konoplev, S.N.; Sweeney, S.R.; et al. Activation of RAS/MAPK Pathway Confers MCL-1 Mediated Acquired Resistance to BCL-2 Inhibitor Venetoclax in Acute Myeloid Leukemia. Signal. Transduct. Target. Ther. 2022, 7, 51. [Google Scholar] [CrossRef]
  57. Stevens, B.M.; Jones, C.L.; Pollyea, D.A.; Culp-Hill, R.; D’Alessandro, A.; Winters, A.; Krug, A.; Abbott, D.; Goosman, M.; Pei, S.; et al. Fatty Acid Metabolism Underlies Venetoclax Resistance in Acute Myeloid Leukemia Stem Cells. Nat. Cancer 2020, 1, 1176–1187. [Google Scholar] [CrossRef]
Ijms 23 10957 g001 550
Figure 1. Interplay of BCL-2 family members in ALL. (a) Venetoclax binds to BCL-2 and displaces Bim and other BH3-only proteins (BAX), promoting activation of Bax and Bak and leading to their oligomerization and mitochondrial outer membrane permeabilization, cytochrome C release and caspase activation. (b) The upregulation of BCL-XL and MCL-1 results in sequestration of these effectors and venetoclax resistance. This can be potentially overcome by co-targeting these molecules as presented. TKI—tyrosine kinase inhibitor, Cyt C—cytochrome C.
Figure 1. Interplay of BCL-2 family members in ALL. (a) Venetoclax binds to BCL-2 and displaces Bim and other BH3-only proteins (BAX), promoting activation of Bax and Bak and leading to their oligomerization and mitochondrial outer membrane permeabilization, cytochrome C release and caspase activation. (b) The upregulation of BCL-XL and MCL-1 results in sequestration of these effectors and venetoclax resistance. This can be potentially overcome by co-targeting these molecules as presented. TKI—tyrosine kinase inhibitor, Cyt C—cytochrome C.
Ijms 23 10957 g001
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Aumann, S.; Shaulov, A.; Haran, A.; Gross Even-Zohar, N.; Vainstein, V.; Nachmias, B. The Emerging Role of Venetoclax-Based Treatments in Acute Lymphoblastic Leukemia. Int. J. Mol. Sci. 2022, 23, 10957. https://doi.org/10.3390/ijms231810957

AMA Style

Aumann S, Shaulov A, Haran A, Gross Even-Zohar N, Vainstein V, Nachmias B. The Emerging Role of Venetoclax-Based Treatments in Acute Lymphoblastic Leukemia. International Journal of Molecular Sciences. 2022; 23(18):10957. https://doi.org/10.3390/ijms231810957

Chicago/Turabian Style

Aumann, Shlomzion, Adir Shaulov, Arnon Haran, Noa Gross Even-Zohar, Vladimir Vainstein, and Boaz Nachmias. 2022. "The Emerging Role of Venetoclax-Based Treatments in Acute Lymphoblastic Leukemia" International Journal of Molecular Sciences 23, no. 18: 10957. https://doi.org/10.3390/ijms231810957

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop