Rapid Evaporative Ionization Mass Spectrometry-Based Lipidomics for Identification of Canine Mammary Pathology
Abstract
:1. Introduction
2. Results
2.1. Mammary Samples
2.2. Database Building and Generation of a Classification Model
2.3. Determination of Discriminant Features
Assigned Compound | Lipid Class | Detected Ion | m/z | Exact Mass | Δ | Note |
---|---|---|---|---|---|---|
Palmitoleic acid (C16:1) | Fatty acid | [M-H]− | 253.2167 | 253.2173 | 2.37 | Healthy |
Palmitic acid (C16:0) | Fatty acid | [M-H]− | 255.2324 | 255.2324 | 0.00 | Tumor/Hyperplasia/Dysplasia > Healthy/Mastitis |
Oleic acid (C18:1) | Fatty acid | [M-H]− | 281.2480 | 281.2480 | 0.00 | Healthy > Hyperplasia/Dysplasia > Tumor > Mastitis |
Stearic acid (C18:0) | Fatty acid | [M-H]− | 283.2636 | 283.2643 | 2.47 | Tumor/Hyperplasia/Dysplasia > Healthy/Mastitis |
Arachidonic acid (C20:4) | Fatty acid | [M-H]− | 303.2333 | 303.2330 | 0.99 | Hyperplasia/Dysplasia/Tumor > Mastitis |
Docosatetraenoic acid (C22:4) | Fatty acid | [M-H]− | 331.2646 | 331.2643 | 0.91 | Tumor |
Lyso-PA(C16:0) | Phospholipid | [M-H2O-H]− | 391.2240 | 391.2250 | 2.56 | Tumor > Hyperplasia/Dysplasia/Mastitis |
PG(C36:1-2OH) * | Phospholipid | [M-2H]2− | 403.2597 | 403.2660 | 15.62 | Mastitis > Tumor/Hyperplasia/Dysplasia |
Lyso-PA(C18:1) | Phospholipid | [M-H2O-H]− | 417.2397 | 417.2406 | 2.16 | Tumor/Hyperplasia/Dysplasia/Mastitis |
Lyso-PA(C18:0) | Phospholipid | [M-H2O-H]− | 419.2555 | 419.2563 | 1.91 | Tumor/Mastitis > Hyperplasia/Dysplasia/Healthy |
Lyso-PA(C20:4) | Phospholipid | [M-H2O-H]− | 439.2247 | 439.2250 | 0.68 | Tumor/Hyperplasia/Dysplasia/Mastitis |
Cer(C34:1) # | Sphingolipid | [M+Cl]− | 572.4811 | 572.4815 | 0.70 | Hyperplasia/Dysplasia > Tumor/Mastitis > Healthy |
PA(C34:1) +† | Phospholipid | [M-H]− | 673.4815 | 673.4814 | 0.15 | Tumor/Mastitis |
SM(d18:1/16:0) + | Sphingolipid | [M-CH3]− | 687.5432 | 687.5334 | 3.35 | Tumor/Hyperplasia/Dysplasia > Mastitis > Healthy |
PA(C36:2) +† | Phospholipid | [M-H]− | 699.4948 | 699.4970 | 3.15 | Tumor/Mastitis > Hyperplasia/Dysplasia > Healthy |
PE(C34:0) PE(O-C34:1) PC(C32:0) | Phospholipid | [M-H]− [M-H]− [M-CH3]− | 718.5389 | 718.5392 | 0.42 | Tumor/Hyperplasia/Dysplasia/Mastitis |
PA(C38:4) | Phospholipid | [M-H]− | 723.4965 | 723.4970 | 0.69 | Mastitis > Tumor/Hyperplasia/Dysplasia |
PE(O-C36:3) | Phospholipid | [M-H]− | 726.5252 | 726.5443 | 13.90 | Healthy > Tumor/Hyperplasia/Dysplasia/Mastitis |
PG 32:0;O * | Phospholipid | [M-H]− | 737.4964 | 737.4974 | 1.36 | Tumor/Mastitis > Hyperplasia/Dysplasia |
PE(C36:1) $† PC(C34:1) +† | Phospholipid | [M-H]− [M-CH3]− | 744.5546 | 744.5549 | 0.40 | Tumor > Hyperplasia/Dysplasia > Mastitis |
PE(O-C38:6) | Phospholipid | [M-H]− | 748.5208 | 748.5287 | 10.55 | Mastitis > Tumor |
PE(O-C38:5) PC(O-36:5) PE(P-38:4) +$ | Phospholipid | [M-H]− [M-CH3]− [M-H]− | 750.5417 | 750.5443 | 3.46 | Tumor/Hyperplasia/Dysplasia/Mastitis > Healthy |
PA(C40:4) | Phospholipid | [M-H]− | 751.5316 | 751.5283 | 4.39 | Tumor/ Hyperplasia/Dysplasia/ Mastitis > Healthy |
PE(C38:4) †$ PC(C36:4) † | Phospholipid | [M-H]− [M-CH3]− | 766.5389 | 766.5392 | 0.39 | Tumor/Hyperplasia/Dysplasia/Mastitis |
PE(C40:4) | Phospholipid | [M-H]− | 794.5692 | 794.5705 | 1.64 | Tumor/Hyperplasia/Dysplasia |
PI(C38:4) #+$ | Phospholipid | [M-H]− | 885.5492 | 885.5499 | 0.79 | Tumor/Mastitis |
TG(C52:3) † | Triglycerid | [M+Cl]− | 891.7150 | 891.7214 | 7.83 | Healthy |
TG(C52:2) † | Triglycerid | [M+Cl]− | 893.7300 | 893.7370 | 6.76 | Healthy |
TG(C54:4) † | Triglycerid | [M+Cl]− | 917.7308 | 917.7370 | 9.57 | Healthy |
TG(C54:3) † | Triglycerid | [M+Cl]− | 919.7439 | 919.7527 | 17.14 | Healthy |
TG(C54:2) † | Triglycerid | [M+Cl]− | 921.7525 | 921.7683 | 0.79 | Healthy |
Assigned Compound | Lipid Class | Detected Ion | m/z | Exact Mass | Δ | Note |
---|---|---|---|---|---|---|
Linoleic acid (C18:2) | Fatty acid | [M-H]− | 279.2326 | 279.2330 | 1.43 | Tumor/Hyperplasia/Dysplasia > Healthy/Mastitis |
Prostaglandin * | Fatty acid derivative | [M+Na-2H]− | 389.1940 | 389.1946 | 1.54 | Hyperplasia/Dysplasia > Tumor |
PGP(C32:4-OH) * | Phospholipid | [M-2H]2− | 404.1968 | 404.1969 | 0.25 | Tumor/Mastitis > Hyperplasia/Dysplasia |
NAT 20:4 | Fatty acid derivative | [M+Cl]− | 446.2122 | 446.2137 | 3.36 | Hyperplasia/Dysplasia > Tumor |
PGP(C38:6-3OH) * | Phospholipid | [M-2H]2− | 460.2281 | 460.2341 | 13.04 | Tumor/Hyperplasia/Dysplasia/Mastitis |
CerP(d18:1/18:1) | Sphingolipid | [M-H]− | 642.4850 | 642.4868 | 2.80 | Mastitis |
PA(C38:3) + | Phospholipid | [M-H]− | 725.5099 | 725.5127 | 3.86 | Healthy > Tumor/Hyperplasia/Dysplasia/Mastitis |
PE(C36:2) † PC(C34:2) † | Phospholipid | [M-H]− [M-CH3]− | 742.5387 | 742.5392 | 0.67 | Tumor > Healthy > Hyperplasia/Dysplasia/Mastitis |
PE(C38:2) † PC(C36:2) † | Phospholipid | [M-H]− [M-CH3]− | 770. 5705 | 770.5705 | 0.00 | Healthy > Tumor/Hyperplasia/Dysplasia/Mastitis |
3. Discussion
4. Materials and Methods
4.1. Samples and Sample Treatment
4.2. Histopathology
4.3. iKnife Analysis
4.3.1. Chemicals
4.3.2. iKnife Instrumentation and Analytical Conditions
4.3.3. Data Processing and Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mangraviti, D.; Abbate, J.M.; Iaria, C.; Rigano, F.; Mondello, L.; Quartuccio, M.; Marino, F. Rapid Evaporative Ionization Mass Spectrometry-Based Lipidomics for Identification of Canine Mammary Pathology. Int. J. Mol. Sci. 2022, 23, 10562. https://doi.org/10.3390/ijms231810562
Mangraviti D, Abbate JM, Iaria C, Rigano F, Mondello L, Quartuccio M, Marino F. Rapid Evaporative Ionization Mass Spectrometry-Based Lipidomics for Identification of Canine Mammary Pathology. International Journal of Molecular Sciences. 2022; 23(18):10562. https://doi.org/10.3390/ijms231810562
Chicago/Turabian StyleMangraviti, Domenica, Jessica Maria Abbate, Carmelo Iaria, Francesca Rigano, Luigi Mondello, Marco Quartuccio, and Fabio Marino. 2022. "Rapid Evaporative Ionization Mass Spectrometry-Based Lipidomics for Identification of Canine Mammary Pathology" International Journal of Molecular Sciences 23, no. 18: 10562. https://doi.org/10.3390/ijms231810562