Next Article in Journal
High Plasma Levels of Fortilin in Patients with Coronary Artery Disease
Next Article in Special Issue
Investigation of the Properties of Linen Fibers and Dressings
Previous Article in Journal
Impacts of Glucose-Dependent Insulinotropic Polypeptide on Orthodontic Tooth Movement-Induced Bone Remodeling
Previous Article in Special Issue
In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin Cells
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 23
Issue 16
10.3390/ijms23168918
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model

by
Jonah Ee Hsiang Kua
1,
Chun Wei Siow
2,
Wee Keng Lim
2,
Jeyakumar Masilamani
3,
Monica Suryana Tjin
4,
Joe Yeong
5,6,
Tony Kiat Hon Lim
6,
Toan Thang Phan
3,7 and
Alvin Wen Choong Chua
1,2,8,*
1
Department of Plastic, Reconstructive and Aesthetic Surgery, Singapore General Hospital, 20 College Road, Singapore 169856, Singapore
2
Skin Bank Unit, Singapore General Hospital, Outram Road, Singapore 169608, Singapore
3
CellResearch Corporation Pte. Ltd., 137 Market Street, Grace Global Raffles #08-02, Singapore 048943, Singapore
4
Programme in Cardiovascular and Metabolic Disorders, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore
5
Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis, Proteos, Singapore 138673, Singapore
6
Department of Anatomical Pathology, Singapore General Hospital, 20 College Road, Singapore 169856, Singapore
7
Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Kent Ridge Road, Singapore 119228, Singapore
8
Musculoskeletal Sciences Academic Clinical Programme, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(16), 8918; https://doi.org/10.3390/ijms23168918
Submission received: 30 June 2022 / Revised: 3 August 2022 / Accepted: 7 August 2022 / Published: 10 August 2022
(This article belongs to the Special Issue Recent Approaches for Wound Treatment)
Browse Figures
Review Reports Versions Notes

Abstract

Human umbilical cord lining epithelial cells [CLECs) are naïve in nature and can be ethically recovered from cords that are routinely discarded. The success of using oral mucosal epithelial cells for cornea defects hints at the feasibility of treating cutaneous wounds using non-native CLECs. Herein, we characterized CLECs using flow cytometry (FC) and skin organotypic cultures in direct comparison with skin keratinocytes (KCs). This was followed by wound healing study to compare the effects of CLEC application and the traditional use of human skin allografts (HSGs) in a porcine wound model. While CLECs were found to express all the epidermal cell markers probed, the major difference between CLECs and KCs lies in the level of expression (in FC analysis) as well as in the location of expression (of the epithelium in organotypic cultures) of some of the basal cell markers probed. On the pig wounds, CLEC application promoted accelerated healing with no adverse reaction compared to HSG use. Though CLECs, like HSGs, elicited high levels of local and systemic immune responses in the animals during the first week, these effects were tapered off more quickly in the CLEC-treated group. Overall, the in vivo porcine data point to the potential of CLECs as a non-native and safe source of cells to treat cutaneous wounds.
Keywords: umbilical cord; cord lining epithelial cells; skin keratinocytes; cutaneous wounds; wound healing; CLECs umbilical cord; cord lining epithelial cells; skin keratinocytes; cutaneous wounds; wound healing; CLECs

Share and Cite

MDPI and ACS Style

Kua, J.E.H.; Siow, C.W.; Lim, W.K.; Masilamani, J.; Tjin, M.S.; Yeong, J.; Lim, T.K.H.; Phan, T.T.; Chua, A.W.C. Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model. Int. J. Mol. Sci. 2022, 23, 8918. https://doi.org/10.3390/ijms23168918

AMA Style

Kua JEH, Siow CW, Lim WK, Masilamani J, Tjin MS, Yeong J, Lim TKH, Phan TT, Chua AWC. Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model. International Journal of Molecular Sciences. 2022; 23(16):8918. https://doi.org/10.3390/ijms23168918

Chicago/Turabian Style

Kua, Jonah Ee Hsiang, Chun Wei Siow, Wee Keng Lim, Jeyakumar Masilamani, Monica Suryana Tjin, Joe Yeong, Tony Kiat Hon Lim, Toan Thang Phan, and Alvin Wen Choong Chua. 2022. "Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model" International Journal of Molecular Sciences 23, no. 16: 8918. https://doi.org/10.3390/ijms23168918

APA Style

Kua, J. E. H., Siow, C. W., Lim, W. K., Masilamani, J., Tjin, M. S., Yeong, J., Lim, T. K. H., Phan, T. T., & Chua, A. W. C. (2022). Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model. International Journal of Molecular Sciences, 23(16), 8918. https://doi.org/10.3390/ijms23168918

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop