The Relationship and Expression of miR-451a, miR-25-3p and PTEN in Early Peritoneal Endometriotic Lesions and Their Modulation In Vitro
Abstract
:1. Introduction
2. Results
2.1. Endometriotic Lesion Expression of PTEN Transcript
2.2. Endometriotic Lesion Expression of miR-25-3p and Relationship with PTEN
2.3. Relationship between miR-451a and miR-25-3p In Vivo
2.4. miR-451a Regulatoin of miR-25-3p In Vitro
2.5. miR-25-3p Down-Regulates PTEN Protein In Vitro
3. Discussion
4. Materials and Methods
4.1. Human Subjects and Tissue Acquisition
4.2. mRNA Isolation and qRT-PCR
4.3. Cell Culture of Endometriotic Epithelial 12Z Cells and Transfection
4.4. Western Analysis
4.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Nothnick, W.B.; Peterson, R.; Minchella, P.; Falcone, T.; Graham, A.; Findley, A. The Relationship and Expression of miR-451a, miR-25-3p and PTEN in Early Peritoneal Endometriotic Lesions and Their Modulation In Vitro. Int. J. Mol. Sci. 2022, 23, 5862. https://doi.org/10.3390/ijms23115862
Nothnick WB, Peterson R, Minchella P, Falcone T, Graham A, Findley A. The Relationship and Expression of miR-451a, miR-25-3p and PTEN in Early Peritoneal Endometriotic Lesions and Their Modulation In Vitro. International Journal of Molecular Sciences. 2022; 23(11):5862. https://doi.org/10.3390/ijms23115862
Chicago/Turabian StyleNothnick, Warren B., Riley Peterson, Paige Minchella, Tommaso Falcone, Amanda Graham, and Austin Findley. 2022. "The Relationship and Expression of miR-451a, miR-25-3p and PTEN in Early Peritoneal Endometriotic Lesions and Their Modulation In Vitro" International Journal of Molecular Sciences 23, no. 11: 5862. https://doi.org/10.3390/ijms23115862
APA StyleNothnick, W. B., Peterson, R., Minchella, P., Falcone, T., Graham, A., & Findley, A. (2022). The Relationship and Expression of miR-451a, miR-25-3p and PTEN in Early Peritoneal Endometriotic Lesions and Their Modulation In Vitro. International Journal of Molecular Sciences, 23(11), 5862. https://doi.org/10.3390/ijms23115862