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Characterisation of Extracellular Vesicles from Equine Mesenchymal Stem Cells

1
VETERM, Equine Surgery Unit, Department for Companion Animals and Horses, Vetmeduni, 1210 Vienna, Austria
2
Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria
3
Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, 1200 Vienna, Austria
4
Institute of Molecular Biotechnology, University of Natural Resources and Life Sciences, 1090 Vienna, Austria
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Takayoshi Yamaza
Int. J. Mol. Sci. 2022, 23(10), 5858; https://doi.org/10.3390/ijms23105858
Received: 7 March 2022 / Revised: 15 May 2022 / Accepted: 17 May 2022 / Published: 23 May 2022
(This article belongs to the Section Molecular Biology)
Extracellular vesicles (EVs) are nanosized lipid bilayer-encapsulated particles secreted by virtually all cell types. EVs play an essential role in cellular crosstalk in health and disease. The cellular origin of EVs determines their composition and potential therapeutic effect. Mesenchymal stem/stromal cell (MSC)-derived EVs have shown a comparable therapeutic potential to their donor cells, making them a promising tool for regenerative medicine. The therapeutic application of EVs circumvents some safety concerns associated with the transplantation of viable, replicating cells and facilitates the quality-controlled production as a ready-to-go, off-the-shelf biological therapy. Recently, the International Society for Extracellular Vesicles (ISEV) suggested a set of minimal biochemical, biophysical and functional standards to define extracellular vesicles and their functions to improve standardisation in EV research. However, nonstandardised EV isolation methods and the limited availability of cross-reacting markers for most animal species restrict the application of these standards in the veterinary field and, therefore, the species comparability and standardisation of animal experiments. In this study, EVs were isolated from equine bone-marrow-derived MSCs using two different isolation methods, stepwise ultracentrifugation and size exclusion chromatography, and minimal experimental requirements for equine EVs were established and validated. Equine EVs were characterised using a nanotracking analysis, fluorescence-triggered flow cytometry, Western blot and transelectron microscopy. Based on the ISEV standards, minimal criteria for defining equine EVs are suggested as a baseline to allow the comparison of EV preparations obtained by different laboratories. View Full-Text
Keywords: extracellular vesicles; equine; mesenchymal stem cell; EV isolation; EV characterisation extracellular vesicles; equine; mesenchymal stem cell; EV isolation; EV characterisation
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MDPI and ACS Style

Soukup, R.; Gerner, I.; Gültekin, S.; Baik, H.; Oesterreicher, J.; Grillari, J.; Jenner, F. Characterisation of Extracellular Vesicles from Equine Mesenchymal Stem Cells. Int. J. Mol. Sci. 2022, 23, 5858. https://doi.org/10.3390/ijms23105858

AMA Style

Soukup R, Gerner I, Gültekin S, Baik H, Oesterreicher J, Grillari J, Jenner F. Characterisation of Extracellular Vesicles from Equine Mesenchymal Stem Cells. International Journal of Molecular Sciences. 2022; 23(10):5858. https://doi.org/10.3390/ijms23105858

Chicago/Turabian Style

Soukup, Robert, Iris Gerner, Sinan Gültekin, Hayeon Baik, Johannes Oesterreicher, Johannes Grillari, and Florien Jenner. 2022. "Characterisation of Extracellular Vesicles from Equine Mesenchymal Stem Cells" International Journal of Molecular Sciences 23, no. 10: 5858. https://doi.org/10.3390/ijms23105858

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