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Article

Graphene Enhances Actin Filament Assembly Kinetics and Modulates NIH-3T3 Fibroblast Cell Spreading

1
NanoScience Technology Center, University of Central Florida, Orlando, FL 32826, USA
2
Department of Materials Science and Engineering, University of Central Florida, Orlando, FL 32816, USA
3
Department of Electrical and Computer Engineering, University of Central Florida, Orlando, FL 32816, USA
4
Department of Physics, University of Central Florida, Orlando, FL 32816, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Ana María Díez-Pascual
Int. J. Mol. Sci. 2022, 23(1), 509; https://doi.org/10.3390/ijms23010509
Received: 18 November 2021 / Revised: 28 December 2021 / Accepted: 30 December 2021 / Published: 3 January 2022
(This article belongs to the Collection Feature Papers in Molecular Biophysics)
Actin plays critical roles in various cellular functions, including cell morphogenesis, differentiation, and movement. The assembly of actin monomers into double-helical filaments is regulated in surrounding microenvironments. Graphene is an attractive nanomaterial that has been used in various biomaterial applications, such as drug delivery cargo and scaffold for cells, due to its unique physical and chemical properties. Although several studies have shown the potential effects of graphene on actin at the cellular level, the direct influence of graphene on actin filament dynamics has not been studied. Here, we investigate the effects of graphene on actin assembly kinetics using spectroscopy and total internal reflection fluorescence microscopy. We demonstrate that graphene enhances the rates of actin filament growth in a concentration-dependent manner. Furthermore, cell morphology and spreading are modulated in mouse embryo fibroblast NIH-3T3 cultured on a graphene surface without significantly affecting cell viability. Taken together, these results suggest that graphene may have a direct impact on actin cytoskeleton remodeling. View Full-Text
Keywords: actin cytoskeleton; assembly kinetics; graphene; excluded area; cell spreading actin cytoskeleton; assembly kinetics; graphene; excluded area; cell spreading
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MDPI and ACS Style

Park, J.; Kravchuk, P.; Krishnaprasad, A.; Roy, T.; Kang, E.H. Graphene Enhances Actin Filament Assembly Kinetics and Modulates NIH-3T3 Fibroblast Cell Spreading. Int. J. Mol. Sci. 2022, 23, 509. https://doi.org/10.3390/ijms23010509

AMA Style

Park J, Kravchuk P, Krishnaprasad A, Roy T, Kang EH. Graphene Enhances Actin Filament Assembly Kinetics and Modulates NIH-3T3 Fibroblast Cell Spreading. International Journal of Molecular Sciences. 2022; 23(1):509. https://doi.org/10.3390/ijms23010509

Chicago/Turabian Style

Park, Jinho, Pavlo Kravchuk, Adithi Krishnaprasad, Tania Roy, and Ellen H. Kang. 2022. "Graphene Enhances Actin Filament Assembly Kinetics and Modulates NIH-3T3 Fibroblast Cell Spreading" International Journal of Molecular Sciences 23, no. 1: 509. https://doi.org/10.3390/ijms23010509

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