The Combination of Bacillus Thuringiensis and Its Engineered Strain Expressing dsRNA Increases the Toxicity against Plutella Xylostella
Abstract
:1. Introduction
2. Results
2.1. Engineered Bt Expressing dsRNA of PxAK
2.2. Toxicity of Engineered Bt against P. xylostella
2.3. Toxicity of the Mixtures of Bt 8010 and Engineered Bt against P. Xylostella
2.4. Growth and Development Characteristics of P. xylostella Feeding on the Mixtures of Bt and Engineered Bt
2.5. Effects of the Mixtures of Bt and Engineered Bt on P. xylostella Population Dynamics Parameters
3. Discussion
4. Materials and Methods
4.1. Insects and Bacteria
4.2. Construction of RNAi vector of PxAK
4.3. Construction of Engineered Bt
4.4. Feeding Bioassay
4.5. Quantification of PxAK Gene Expression
4.6. Life Table Analysis of Laboratory Population
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Intrinsic Rate of Increase, r (d−1) | Finite Rate of Increase, λ (d−1) | Net Reproductive Rate, R0 (Offspring) | Mean Generation Time, T (d) |
---|---|---|---|---|
H2O | 0.2427 ± 0.0090 ab | 1.2746 ± 0.0115 ab | 70.4682 ± 10.1416 ab | 17.5360 ± 0.1570 b |
8010 | 0.2529 ± 0.0107 a | 1.2878 ± 0.0137 a | 69.7647 ± 10.9253 ab | 16.7840 ± 0.2340 c |
8010AKi | 0.2510 ± 0.0089 a | 1.2852 ± 0.0114 a | 81.9904 ± 11.3744 a | 17.5590 ± 0.2170 b |
8010:8010AKi of 1:9 | 0.2065 ± 0.0115 c | 1.2293 ± 0.0141 c | 45.9127 ± 8.9724 bc | 18.5360 ± 0.2660 a |
8010:8010AKi of 3:7 | 0.2153 ± 0.0117 bc | 1.2403 ± 0.0145 bc | 52.2844 ± 10.1531 abc | 18.3760 ± 0.2280 a |
8010:8010AKi of 5:5 | 0.2383 ± 0.0115 abc | 1.2691 ± 0.0146 abc | 66.1250 ± 12.3838 abc | 17.5920 ± 0.2250 b |
8010:8010AKi of 7:3 | 0.2145 ± 0.0099 c | 1.2393 ± 0.0122 c | 56.4240 ± 9.3667 abc | 18.7990 ± 0.2530 a |
8010:8010AKi of 9:1 | 0.2097 ± 0.0147 c | 1.2333 ± 0.0180 c | 39.8571 ± 9.3817 c | 17.5760 ± 0.2460 b |
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Jiang, Y.-X.; Chen, J.-Z.; Li, M.-W.; Zha, B.-H.; Huang, P.-R.; Chu, X.-M.; Chen, J.; Yang, G. The Combination of Bacillus Thuringiensis and Its Engineered Strain Expressing dsRNA Increases the Toxicity against Plutella Xylostella. Int. J. Mol. Sci. 2022, 23, 444. https://doi.org/10.3390/ijms23010444
Jiang Y-X, Chen J-Z, Li M-W, Zha B-H, Huang P-R, Chu X-M, Chen J, Yang G. The Combination of Bacillus Thuringiensis and Its Engineered Strain Expressing dsRNA Increases the Toxicity against Plutella Xylostella. International Journal of Molecular Sciences. 2022; 23(1):444. https://doi.org/10.3390/ijms23010444
Chicago/Turabian StyleJiang, Ying-Xia, Jin-Zhi Chen, Miao-Wen Li, Ben-Hu Zha, Peng-Rong Huang, Xue-Mei Chu, Jing Chen, and Guang Yang. 2022. "The Combination of Bacillus Thuringiensis and Its Engineered Strain Expressing dsRNA Increases the Toxicity against Plutella Xylostella" International Journal of Molecular Sciences 23, no. 1: 444. https://doi.org/10.3390/ijms23010444