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Article

Alpha-Helical Protein KfrC Acts as a Switch between the Lateral and Vertical Modes of Dissemination of Broad-Host-Range RA3 Plasmid from IncU (IncP-6) Incompatibility Group

1
Laboratory of DNA Segregation and Cell Cycle of Proteobacteria, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland
2
Faculty of Chemistry, Chair of Drug and Cosmetics Biotechnology, Warsaw University of Technology, 00-664 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Baltasar Mayo
Int. J. Mol. Sci. 2021, 22(9), 4880; https://doi.org/10.3390/ijms22094880
Received: 31 March 2021 / Revised: 27 April 2021 / Accepted: 3 May 2021 / Published: 5 May 2021
(This article belongs to the Section Molecular Microbiology)
KfrC proteins are encoded by the conjugative broad-host-range plasmids that also encode alpha-helical filament-forming KfrA proteins as exemplified by the RA3 plasmid from the IncU incompatibility group. The RA3 variants impaired in kfrA, kfrC, or both affected the host’s growth and demonstrated the altered stability in a species-specific manner. In a search for partners of the alpha-helical KfrC protein, the host’s membrane proteins and four RA3-encoded proteins were found, including the filamentous KfrA protein, segrosome protein KorB, and the T4SS proteins, the coupling protein VirD4 and ATPase VirB4. The C-terminal, 112-residue dimerization domain of KfrC was involved in the interactions with KorB, the master player of the active partition, and VirD4, a key component of the conjugative transfer process. In Pseudomonas putida, but not in Escherichia coli, the lack of KfrC decreased the stability but improved the transfer ability. We showed that KfrC and KfrA were involved in the plasmid maintenance and conjugative transfer and that KfrC may play a species-dependent role of a switch between vertical and horizontal modes of RA3 spreading. View Full-Text
Keywords: alpha-helical KfrC protein; broad-host-range RA3 plasmid; IncU (IncP-6) group; active partition; conjugative transfer alpha-helical KfrC protein; broad-host-range RA3 plasmid; IncU (IncP-6) group; active partition; conjugative transfer
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MDPI and ACS Style

Mitura, M.; Lewicka, E.; Godziszewska, J.; Adamczyk, M.; Jagura-Burdzy, G. Alpha-Helical Protein KfrC Acts as a Switch between the Lateral and Vertical Modes of Dissemination of Broad-Host-Range RA3 Plasmid from IncU (IncP-6) Incompatibility Group. Int. J. Mol. Sci. 2021, 22, 4880. https://doi.org/10.3390/ijms22094880

AMA Style

Mitura M, Lewicka E, Godziszewska J, Adamczyk M, Jagura-Burdzy G. Alpha-Helical Protein KfrC Acts as a Switch between the Lateral and Vertical Modes of Dissemination of Broad-Host-Range RA3 Plasmid from IncU (IncP-6) Incompatibility Group. International Journal of Molecular Sciences. 2021; 22(9):4880. https://doi.org/10.3390/ijms22094880

Chicago/Turabian Style

Mitura, Monika; Lewicka, Ewa; Godziszewska, Jolanta; Adamczyk, Malgorzata; Jagura-Burdzy, Grazyna. 2021. "Alpha-Helical Protein KfrC Acts as a Switch between the Lateral and Vertical Modes of Dissemination of Broad-Host-Range RA3 Plasmid from IncU (IncP-6) Incompatibility Group" Int. J. Mol. Sci. 22, no. 9: 4880. https://doi.org/10.3390/ijms22094880

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