Marliolide Derivative Induces Melanosome Degradation via Nrf2/p62-Mediated Autophagy
Abstract
:1. Introduction
2. Results
2.1. Marliolide or Marliolide Derivatives Reduced Melanin Products in B16F0 Melanoma Cells
2.2. DMF02 Reduced UVB-Induced Pigmentation in HRM-2 Mice Skin
2.3. DMF02 Decreased Melanin Production in Melanocytes and Keratinocytes Containing Transferred Melanosomes
2.4. DMF02 Decreased Melanosome in Melanocytes and Keratinocytes
2.5. DMF02 Increased Autophagy in HRM-2 Mice Skin
2.6. DMF02 Reduced Melanin-Containing Melanosome via Autophagy in Melanocytes and Keratinocytes
2.7. Nrf2 Knockdown Decreased DMF02-Induced Autophagy, Blocking Melanosome Degradation in Melanocytes
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Cell Culture
4.3. Melanin Quantification
4.4. UVB-Induced Skin Hyperpigmentation
4.5. Isolation of Melanosomes and Incubation of Isolated Melanosomes in HaCaT Cells
4.6. Transfection and Plasmids
4.7. Western Blot Analysis
4.8. Real-Time PCR Analysis
4.9. Small Interfering RNA Knockdown
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yun, C.-Y.; Choi, N.; Lee, J.U.; Lee, E.J.; Kim, J.Y.; Choi, W.J.; Oh, S.H.; Sung, J.-H. Marliolide Derivative Induces Melanosome Degradation via Nrf2/p62-Mediated Autophagy. Int. J. Mol. Sci. 2021, 22, 3995. https://doi.org/10.3390/ijms22083995
Yun C-Y, Choi N, Lee JU, Lee EJ, Kim JY, Choi WJ, Oh SH, Sung J-H. Marliolide Derivative Induces Melanosome Degradation via Nrf2/p62-Mediated Autophagy. International Journal of Molecular Sciences. 2021; 22(8):3995. https://doi.org/10.3390/ijms22083995
Chicago/Turabian StyleYun, Cheong-Yong, Nahyun Choi, Jae Un Lee, Eun Jung Lee, Ji Young Kim, Won Jun Choi, Sang Ho Oh, and Jong-Hyuk Sung. 2021. "Marliolide Derivative Induces Melanosome Degradation via Nrf2/p62-Mediated Autophagy" International Journal of Molecular Sciences 22, no. 8: 3995. https://doi.org/10.3390/ijms22083995