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Article

One Heat Shock Transcription Factor Confers High Thermal Tolerance in Clematis Plants

by 1,2,†, 1,2,†, 3,†, 1,2, 1,2, 1,2, 3,* and 1,2,*
1
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
2
The Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
3
Shanghai Botanical Garden, Shanghai Urban Plant Resources Development and Application Engineering Technology Research Center, Shanghai 200231, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Gabriella Szalai
Int. J. Mol. Sci. 2021, 22(6), 2900; https://doi.org/10.3390/ijms22062900
Received: 3 March 2021 / Accepted: 8 March 2021 / Published: 12 March 2021
(This article belongs to the Section Molecular Plant Sciences)
Clematis plants play an important role in botanical gardens. Heat stress can destroy the activity, state and conformation of plant proteins, and its regulatory pathway has been well characterized in Arabidopsis and some crop plants. However, the heat resistance response mechanism in horticultural plants including Clematis has rarely been reported. Here, we identified a heat-tolerant clematis species, Clematis vitalba. The relative water loss and electrolytic leakage were significantly lower under heat treatment in Clematis vitalba compared to Stolwijk Gold. Differential expression heat-tolerant genes (HTGs) were identified based on nonparametric transcriptome analysis. For validation, one heat shock transcription factor, CvHSF30-2, extremely induced by heat stimuli in Clematis vitalba, was identified to confer tolerance to heat stress in Escherichia coli and Saccharomyces cerevisiae. Furthermore, silencing of HSF30-2 by virus-induced gene silencing (VIGS) led to heat sensitivity in tobacco and Clematis, suggesting that the candidate heat-resistant genes identified in this RNA-seq analysis are credible and offer significant utility. We also found that CvHSF30-2 improved heat tolerance of Clematis vitalba by elevating heat shock protein (HSP) expression, which was negatively regulated by CvHSFB2a. Taken together, this study provides insights into the mechanism of Clematis heat tolerance and the findings can be potentially applied in horticultural plants to improve economic efficiency through genetic approaches. View Full-Text
Keywords: Clematis; heat stress; transcriptome analysis; CvHSF30-2; CvHSFB2a; VIGS Clematis; heat stress; transcriptome analysis; CvHSF30-2; CvHSFB2a; VIGS
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MDPI and ACS Style

Wang, R.; Mao, C.; Jiang, C.; Zhang, L.; Peng, S.; Zhang, Y.; Feng, S.; Ming, F. One Heat Shock Transcription Factor Confers High Thermal Tolerance in Clematis Plants. Int. J. Mol. Sci. 2021, 22, 2900. https://doi.org/10.3390/ijms22062900

AMA Style

Wang R, Mao C, Jiang C, Zhang L, Peng S, Zhang Y, Feng S, Ming F. One Heat Shock Transcription Factor Confers High Thermal Tolerance in Clematis Plants. International Journal of Molecular Sciences. 2021; 22(6):2900. https://doi.org/10.3390/ijms22062900

Chicago/Turabian Style

Wang, Rui, Chanjuan Mao, Changhua Jiang, Long Zhang, Siyuan Peng, Yi Zhang, Shucheng Feng, and Feng Ming. 2021. "One Heat Shock Transcription Factor Confers High Thermal Tolerance in Clematis Plants" International Journal of Molecular Sciences 22, no. 6: 2900. https://doi.org/10.3390/ijms22062900

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