Bioluminescence Measurement of Time-Dependent Dynamic Changes of CYP-Mediated Cytotoxicity in CYP-Expressing Luminescent HepG2 Cells
Abstract
:1. Introduction
2. Results
2.1. Generation of CYP-Expressing Luminescent HepG2 Cells
2.2. Comparison of Sensitivity of ELuc-HepG2 and CYPs-ELuc HepG2 Cells to CYP-Independent Toxicants
2.3. CYP-Mediated Cytotoxicity in CYPs-ELuc-HepG2 Cells
2.4. Stability of CYP-Mediated Cytotoxicity in CYPs-ELuc-HepG2 Cells during Long-Term Culture
2.5. Real-Time Bioluminescence Measurement of CYP-Mediated Cytotoxicity in CYPs-ELuc-HepG2 Cells
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Cell Culture
4.3. Microcell-Mediated Chromosome Transfer (MMCT)
4.4. FISH Analysis
4.5. Genomic PCR
4.6. Measurement of CYP Activities
4.7. Non-Destructive Bioluminescence Measurement and WST-1 Assay
4.8. Real-Time Bioluminescence Measurement
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Iwado, S.; Abe, S.; Oshimura, M.; Kazuki, Y.; Nakajima, Y. Bioluminescence Measurement of Time-Dependent Dynamic Changes of CYP-Mediated Cytotoxicity in CYP-Expressing Luminescent HepG2 Cells. Int. J. Mol. Sci. 2021, 22, 2843. https://doi.org/10.3390/ijms22062843
Iwado S, Abe S, Oshimura M, Kazuki Y, Nakajima Y. Bioluminescence Measurement of Time-Dependent Dynamic Changes of CYP-Mediated Cytotoxicity in CYP-Expressing Luminescent HepG2 Cells. International Journal of Molecular Sciences. 2021; 22(6):2843. https://doi.org/10.3390/ijms22062843
Chicago/Turabian StyleIwado, Satoru, Satoshi Abe, Mitsuo Oshimura, Yasuhiro Kazuki, and Yoshihiro Nakajima. 2021. "Bioluminescence Measurement of Time-Dependent Dynamic Changes of CYP-Mediated Cytotoxicity in CYP-Expressing Luminescent HepG2 Cells" International Journal of Molecular Sciences 22, no. 6: 2843. https://doi.org/10.3390/ijms22062843